Identification of brain structures and blood vessels by conventional ultrasound in rats

Frutos, Mari Carmen Gómez-de; García-Suárez, Iván; Laso-García, Fernando; Diekhorst, Luke; Otero-Ortega, Laura; Alonso-López, Elisa; Díez‐Tejedor, Exuperio; Gutiérrez-Fernández, María; Ruíz-Ares, Gerardo

doi:10.1016/j.jneumeth.2020.108935

Cited by 13 publications

(13 citation statements)

References 31 publications

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“…In later phases, brain atrophy occurs with atrophy of the caudate and enlargement of the ipsilateral ventricle ( 52 , 53 ). In a previous study, we had demonstrated the ability of B-mode ultrasound imaging to identify fluid-filled cavities and to differentiate them from brain tissue in rats ( 26 ). We have also identified the subarachnoid cisterns as the main structures that can be observed.…”

Section: Discussionmentioning

confidence: 99%

“…ICH was identified with hyperechogenic appearance at 48 h and hypoechoic appearance at 1 mo. To study the displacement of the cerebral structures, the subarachnoid cisterns were used as a reference ( 26 ). For this purpose, the transducer was placed perpendicular and caudal to the head.…”

Section: Methodsmentioning

confidence: 99%

“…For both the B-mode ultrasound and MRI studies, the AxBxC/2 formula ( 27 , 28 ) was employed for the ICH volume, and brain structure displacement was studied using as reference the distance between the subarachnoid cisterns and the dura mater previously identified ( 26 ). In order to identify the displacement and magnitude of this, the distance from the dura mater to the subarachnoid cisterns was measured, and the cistern displacement ratio (CDR), to normalize and refer to the contralateral side, was calculated with the following formula: cisterns to dura mater distance on the side ipsilateral to the lesion/cisterns to dura mater distance on the side contralateral to the lesion.…”

Section: Methodsmentioning

confidence: 99%

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B-Mode Ultrasound, a Reliable Tool for Monitoring Experimental Intracerebral Hemorrhage

et al. 2021

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Background: Magnetic resonance imaging (MRI) is currently used for the study of intracerebral hemorrhage (ICH) in animal models. However, ultrasound is an inexpensive, non-invasive and rapid technique that could facilitate the diagnosis and follow-up of ICH. This study aimed to evaluate the feasibility and reliability of B-mode ultrasound as an alternative tool for in vivo monitoring of ICH volume and brain structure displacement in an animal model.Methods: A total of 31 male and female Sprague-Dawley rats were subjected to an ICH model using collagenase-IV in the striatum following stereotaxic references. The animals were randomly allocated into 3 groups: healthy (n = 10), sham (n = 10) and ICH (n = 11). B-mode ultrasound studies with a 13-MHz probe were performed pre-ICH and at 5 h, 48 h, 4 d and 1 mo post-ICH for the assessment of ICH volume and displacement of brain structures, considering the distance between the subarachnoid cisterns and the dura mater. The same variables were studied by MRI at 48 h and 1 mo post-ICH.Results: Both imaging techniques showed excellent correlation in measuring ICH volume at 48 h (r = 0.905) and good at 1 mo (r = 0.656). An excellent correlation was also observed in the measured distance between the subarachnoid cisterns and the dura mater at 1 mo between B-mode ultrasound and MRI, on both the ipsilateral (r = 0.870) and contralateral (r = 0.906) sides of the lesion.Conclusion: B-mode ultrasound imaging appears to be a reliable tool for in vivo assessment of ICH volume and displacement of brain structures in animal models.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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B-Mode Ultrasound, a Reliable Tool for Monitoring Experimental Intracerebral Hemorrhage

et al. 2021

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“…Moreover, imaging ultrasound was also applied in an animal model of ICH using 13–14 MHz transcranial Doppler ultrasonography and did not report secondary hemorrhages [ 37 ]. Other studies examined the brain’s blood flow and anatomical structures in healthy animals using a 13 MHz linear transcranial transducer [ 61 ] and 16 MHz transducer [ 62 ]. Considering these studies, the range of 12–16 MHz frequencies to be safe for imaging in the animal brain.…”

Section: Safety Of Ultrasoundmentioning

confidence: 99%

“…The information on brain anatomy provided by ultrasound may help research on neurological diseases. B-mode echography was proven as a feasible tool for noninvasively studying anatomical structures of the rat brain [ 61 ] and, Superb Microvascular Imaging (SMI), an advanced and innovative ultrasound mode based on Doppler, allows study of microvascular blood flow with and without contrast agents, enabling the creation of an innovative vascular brain map [ 61 ].…”

Section: Limitations and Future Of Ultrasoundmentioning

confidence: 99%

The Role of Ultrasound as a Diagnostic and Therapeutic Tool in Experimental Animal Models of Stroke: A Review

et al. 2021

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Ultrasound is a noninvasive technique that provides real-time imaging with excellent resolution, and several studies demonstrated the potential of ultrasound in acute ischemic stroke monitoring. However, only a few studies were performed using animal models, of which many showed ultrasound to be a safe and effective tool also in therapeutic applications. The full potential of ultrasound application in experimental stroke is yet to be explored to further determine the limitations of this technique and to ensure the accuracy of translational research. This review covers the current status of ultrasound applied to monitoring and treatment in experimental animal models of stroke and examines the safety, limitations, and future perspectives.

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Ultrasound‐guided puncture into newborn rat brain

Pao

Zhang

et al. 2023

Ibrain

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Since the brain structure of neonatal rats was not fully formed during the first 4 days, it cannot be detected using ultrasound. The objective of this study was to investigate the use of ultrasound to detect changes in the normal coronal brain structure and determine the puncture depth of the location of the cortex, hippocampus, lateral ventricle, and striatum of newborn Sprague–Dawley (SD) rats of 5−15 days. The animal was placed in a prone position. The specific positions of the cortex, hippocampus, lateral ventricle, and striatum were measured under ultrasound. Then, the SD rats were punctured with a stereotaxic instrument, and dye was injected. Finally, the brains of SD rats were taken to make frozen sections to observe the puncture results. By ultrasound, the image of the cortex, hippocampus, lateral ventricle, and striatum of the rat can be obtained and the puncture depth of the cortex (8 days: 1.02 ± 0.12, 10 days: 1.02 ± 0.08, 13 days: 1.43 ± 0.05), hippocampus (8 days: 2.63 ± 0.07, 10 days: 2.77 ± 0.14, 13 days: 2.82 ± 0.09), lateral ventricle (8 days: 2.08 ± 0.04, 10 days: 2.26 ± 0.03, 13 days: 2.40 ± 0.06), and corpus striatum (8 days: 4.57 ± 0.09, 10 days: 4.94 ± 0.31, 13 days: 5.13 ± 0.10) can be accurately measured. The rat brain structure and puncture depth changed with the age of the rats. Ultrasound technology can not only clarify the brain structure characteristics of 5−15‐d rats but also guide the puncture and injection of the rat brain structure. The results of this study laid the foundation for the future use of ultrasound in experimental animal models of neurological diseases.

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Identification of brain structures and blood vessels by conventional ultrasound in rats

Cited by 13 publications

References 31 publications

B-Mode Ultrasound, a Reliable Tool for Monitoring Experimental Intracerebral Hemorrhage

B-Mode Ultrasound, a Reliable Tool for Monitoring Experimental Intracerebral Hemorrhage

The Role of Ultrasound as a Diagnostic and Therapeutic Tool in Experimental Animal Models of Stroke: A Review

Ultrasound‐guided puncture into newborn rat brain

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