Identification of Brachyspira pilosicoli, Brachyspira hyodysenteriae and Brachyspira intermedia in commercial laying hens and commercial broiler breeders using fluorescence in situ hybridization (FISH) in paraffin-embedded tissues
Leonardo Gruchouskei,
Ronaldo José Piccoli,
Jessica Santos Streauslin
et al.
Abstract:Bacteria of the genus Brachyspira can cause enteric diseases in birds; thus, this study evaluated the efficacy of the fluorescence in situ hybridization (FISH) technique for the identification of B. pilosicoli, B. hyodysenteriae and B. intermedia using cecum samples fixed in formaldehyde from laying hens and commercial broiler breeders. Samples were collected from 112 birds aged between 35 and 82 weeks that originated from commercial laying and broilers farms. For the initial evaluation, spirochaetes were isol… Show more
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