Identification of BP16 as a non-toxic cell-penetrating peptide with highly efficient drug delivery properties

Soler, Marta; González-Bártulos, Marta; Soriano-Castell, David; Ribas, Xavi; Costas, Miguel; Tebar, Francesc; Massaguer, Anna; Feliu, Lidia; Planas, Marta

doi:10.1039/c3ob42422g

Cited by 30 publications

(25 citation statements)

References 68 publications

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“…Soler et al investigated the ability of several AMP variants to act as delivery vehicles for the cytostatic drug chlorambucil [ 57 ]. A set of peptides selected from a library of cecropin-melittin hybrids (CECMEL11), previously designed to be used in plant protection, was screened for their cytotoxicity against diverse cancer cell lines.…”

Section: Modified Amps and Amp Conjugatesmentioning

confidence: 99%

Design and Application of Antimicrobial Peptide Conjugates

Reinhardt

Neundorf

2016

IJMS

119

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Antimicrobial peptides (AMPs) are an interesting class of antibiotics characterized by their unique antibiotic activity and lower propensity for developing resistance compared to common antibiotics. They belong to the class of membrane-active peptides and usually act selectively against bacteria, fungi and protozoans. AMPs, but also peptide conjugates containing AMPs, have come more and more into the focus of research during the last few years. Within this article, recent work on AMP conjugates is reviewed. Different aspects will be highlighted as a combination of AMPs with antibiotics or organometallic compounds aiming to increase antibacterial activity or target selectivity, conjugation with photosensitizers for improving photodynamic therapy (PDT) or the attachment to particles, to name only a few. Owing to the enormous resonance of antimicrobial conjugates in the literature so far, this research topic seems to be very attractive to different scientific fields, like medicine, biology, biochemistry or chemistry.

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Section: Modified Amps and Amp Conjugatesmentioning

confidence: 99%

Design and Application of Antimicrobial Peptide Conjugates

Reinhardt

Neundorf

2016

IJMS

119

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“…Some of the CECMEL11 peptides (e.g. BP100, BP16) had cell penetrating properties in eukaryotic cells and have already been used to deliver conjugated “cargo” compounds intracellularly [12, 13]. Thus, CECMEL11 peptides represent useful tools in different fields of application.…”

Section: Introductionmentioning

confidence: 99%

Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm

et al. 2017

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BackgroundBP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. Here we report the production and recovery of a bioactive BP178 peptide using rice seeds as biofactories.ResultsA synthetic gene encoding the BP178 peptide was prepared and introduced in rice plants. The gene was efficiently expressed in transgenic rice under the control of an endosperm-specific promoter. Among the three endosperm-specific rice promoters (Glutelin B1, Glutelin B4 or Globulin 1), best results were obtained when using the Globulin 1 promoter. The BP178 peptide accumulated in the seed endosperm and was easily recovered from rice seeds using a simple procedure with a yield of 21 μg/g. The transgene was stably inherited for at least three generations, and peptide accumulation remained stable during long term storage of transgenic seeds. The purified peptide showed in vitro activity against the bacterial plant pathogen Dickeya sp., the causal agent of the dark brown sheath rot of rice. Seedlings of transgenic events showed enhanced resistance to the fungal pathogen Fusarium verticillioides, supporting that the in planta produced peptide was biologically active.ConclusionsThe strategy developed in this work for the sustainable production of BP178 peptide using rice seeds as biofactories represents a promising system for future production of peptides for plant protection and possibly in other fields.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1011-9) contains supplementary material, which is available to authorized users.

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“…They frequently take the form of peptides with unnatural amino acids in the chain, most commonly as functionalized Asp, Glu, Lys, or especially Cys residues. Peptide/small molecule adducts are made for a variety of reasons including proteaseresistance [1][2][3][4], improved delivery to cells [5][6][7][8][9], catalysis [8][9][10][11][12][13], fluorescent signaling, and to improve binding affinity [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Captides: rigid junctions between beta sheets and small molecules

Kier

Andersen

2014

J. Pept. Sci.

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An extensive series of covalently linked small molecule-peptide adducts based on a terminally capped beta hairpin motif is reported. The constructs can be prepared by standard solid-phase fmoc chemistry with 1 to 4 peptide chains linked to small molecule hubs bearing carboxylic acid moieties. The key feature of interest is the precise, buried environment of the small molecule, and its rigid orientation relative to one or more short, but fully structured peptide chain(s). Most of this study employs a minimalist 9 residue “captide”, a capped β-turn, but we illustrate general applicability to peptides which can terminate in a beta strand. The non-peptide portion of these adducts can include nearly any molecule bearing one or more carboxylic acid groups. Fold-dependent rigidity sets this strategy apart from currently available bioconjugation methods, which typically engender significant flexibility between peptide and tag. Applications to catalyst enhancement, drug design, higher-order assembly, and FRET calibration rulers are discussed.

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Identification of BP16 as a non-toxic cell-penetrating peptide with highly efficient drug delivery properties

Cited by 30 publications

References 68 publications

Design and Application of Antimicrobial Peptide Conjugates

Design and Application of Antimicrobial Peptide Conjugates

Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm

Captides: rigid junctions between beta sheets and small molecules

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