Identification of bovine prolactin in seminal fluid, and expression and localization of the prolactin receptor and prolactin-inducible protein in the testis and epididymis of bulls exposed to ergot alkaloids

Pratt, Scott L.; Calcatera, Samantha M.; Stowe, Heather M.; Dimmick, M.A.; Schrick, F. N.; Duckett, S. K.; Andrae, John

doi:10.1016/j.theriogenology.2014.10.031

Cited by 11 publications

(4 citation statements)

References 48 publications

(77 reference statements)

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“…PRLR is reportedly present in several tissues where prolactin is known to exert its effects, including the heart, liver, kidney, ovary, prostate, adrenal gland and testes (Chiu et al., 1992). As expected, we found that PRLR was expressed in the yak kidney, liver, testicular, lung, skeletal muscle, heart and spleen and was consistent with observations reported for other species (Guillaumot et al., 1996; Lincoln et al., 2001; Pratt et al., 2015). In addition, PRLR was expressed in the Leydig cells and spermatogonia, which was consistent with the previous reports (Exbrayat & Morel, 2003; Hondo et al., 1995) and indicated that certain types of PRL affected spermatogenesis.…”

Section: Discussionsupporting

confidence: 92%

Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak

et al. 2021

Reprod Domestic Animals

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Luteinizing hormone receptor (LHR), prolactin receptor (PRLR), growth hormone (GH) and insulin‐like growth factor 1 (IGF1) have been shown to be key regulators of germ cell development. However, the role of LHR, PRLR, GH and IGF1 in the development of yak testis remains unclear. In this study, we aimed to describe and compare gene expression and protein localization of LHR, PRLR, GH and IGF1 in the development of yak testes. Testes were collected from 6, 24, 36 and 72 months yak, and the kidney, liver, testicular, lung, skeletal muscle, heart and spleen tissues were collected from 36 months yak. The quantitative real‐time PCR (qRT‐PCR) results showed that the expression of these four genes was widely expressed in kidney, liver, testicular, lung, skeletal muscle, heart and spleen, while the LHR and PRLR were highly expressed in the kidney, skeletal muscle and testis, and higher levels of GH and IGF were expressed in spleen and testis. Moreover, the mRNA expression of these genes in adults was higher than in pre‐pubertal yak. In the testis, the LHR‐, PRLR‐, GH‐ and IGF1‐positive signals were detected in the Leydig cells of the 6 months, while the intense positive signals were discovered in Leydig cells, spermatogonia and spermatocytes of the 36 and 72 months. Thus, LHR, PRLR, GH and IGF1 may be involved in the development of spermatids and spermatocytes, and in the regulation of spermatogonia proliferation and Leydig cell function.

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Section: Discussionsupporting

confidence: 92%

Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak

et al. 2021

Reprod Domestic Animals

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“… 51 discovered SPEF2 (BTA20: 38.08–39.08 window) gene expression in the testes and sperm is regulated by alternative splicing and is thus, one of the determining factors of sperm motility. Lastly, PRLR (BTA20: 38.08–39.08 window) gene expression was identified in the bovine reproductive tract including, the testis, epididymis, spermatogonia, and differentiating germ cells, leading researchers to believe it may have an effect on spermatogenesis 52 . This gene was also found to play a role in fertilization and survival rates through SNP-SNP interactions 53 .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study to identify genomic regions and positional candidate genes associated with male fertility in beef cattle

Sweett

Fonseca

Suárez-Vega

et al. 2020

Sci Rep

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Fertility plays a key role in the success of calf production, but there is evidence that reproductive efficiency in beef cattle has decreased during the past half-century worldwide. Therefore, identifying animals with superior fertility could significantly impact cow-calf production efficiency. The objective of this research was to identify candidate regions affecting bull fertility in beef cattle and positional candidate genes annotated within these regions. A GWAS using a weighted single-step genomic BLUP approach was performed on 265 crossbred beef bulls to identify markers associated with scrotal circumference (SC) and sperm motility (SM). Eight windows containing 32 positional candidate genes and five windows containing 28 positional candidate genes explained more than 1% of the genetic variance for SC and SM, respectively. These windows were selected to perform gene annotation, QTL enrichment, and functional analyses. Functional candidate gene prioritization analysis revealed 14 prioritized candidate genes for SC of which MAP3K1 and VIP were previously found to play roles in male fertility. A different set of 14 prioritized genes were identified for SM and five were previously identified as regulators of male fertility (SOD2, TCP1, PACRG, SPEF2, PRLR). Significant enrichment results were identified for fertility and body conformation QTLs within the candidate windows. Gene ontology enrichment analysis including biological processes, molecular functions, and cellular components revealed significant GO terms associated with male fertility. The identification of these regions contributes to a better understanding of fertility associated traits and facilitates the discovery of positional candidate genes for future investigation of causal mutations and their implications.

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“…In addition to reduced motility, sperm collected from bulls consuming ergot alkaloid-containing pasture also had a slower velocity [ 128 ]. The presence of the prolactin receptor in the testes and the presence of prolactin in seminal fluid has been demonstrated in cattle [ 129 ]. Given the effects of ergot alkaloids on prolactin in the pituitary, this may be an area where ergot alkaloids could have an effect on male reproduction.…”

Section: Decreased Animal Productivitymentioning

confidence: 99%

Activities and Effects of Ergot Alkaloids on Livestock Physiology and Production

Klotz

2015

Toxins

133

141

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Consumption of feedstuffs contaminated with ergot alkaloids has a broad impact on many different physiological mechanisms that alters the homeostasis of livestock. This change in homeostasis causes an increased sensitivity in livestock to perturbations in the ambient environment, resulting in an increased sensitivity to such stressors. This ultimately results in large financial losses in the form of production losses to livestock producers around the world. This review will focus on the underlying physiological mechanisms that are affected by ergot alkaloids that lead to decreases in livestock production.

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Identification of bovine prolactin in seminal fluid, and expression and localization of the prolactin receptor and prolactin-inducible protein in the testis and epididymis of bulls exposed to ergot alkaloids

Cited by 11 publications

References 48 publications

Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak

Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak

Genome-wide association study to identify genomic regions and positional candidate genes associated with male fertility in beef cattle

Activities and Effects of Ergot Alkaloids on Livestock Physiology and Production

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