Identification of Bombyx mori sericin 4 protein as a new biological adhesive

Dong, Zhaoming; Guo, Kaiyu; Zhang, Xiaolu; Zhang, Tong; Zhang, Yan; Ma, Sanyuan; Chang, Huaipu; Tang, Muya; An, Lingna; Xia, Qingyou

doi:10.1016/j.ijbiomac.2019.03.166

Cited by 48 publications

(32 citation statements)

References 28 publications

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“…Therefore, our transcriptome data provide more precise gene predictions. In the case of Sericin-4 , which was recently identified (Dong et al, 2019), we found a longer transcript in our transcriptome data than the gene model reported by Dong et al (2019), which may contribute to the further characterization of sericin-4. The new isoform of Sericin-1 contains CDS that code glycine-, asparagine-, and threonine-rich regions.…”

Section: Discussionsupporting

confidence: 55%

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Reference transcriptome data in silkwormBombyx mori

Yokoi

Tsubota

Sun

et al. 2019

Preprint

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The silkworm Bombyx mori has long been used in the silk industry and utilized in studies of physiology, genetics, molecular biology, and pathology. We recently reported high quality reference genome data for B. mori. In the present study, we constructed a reference transcriptome data set using the reference genome data and RNA-seq data of 10 tissues from P50T strain larvae. Reference transcriptome data contained 51,926 transcripts, with 39,619 having one or more coding sequence region. The abundance of each transcript in the 10 tissues as well as 5 tissues from other strain larvae was estimated, and hierarchical clustering was performed. The results obtained showed that data on abundance were highly reproducible and there here is a little difference of transcriptome abundance between the two strain larvae. New isoforms of silk-related genes were searched for in the reference transcriptomes, and the longest isoform of sericin-1 possessing a long exon was identified. We also extracted transcripts that were strongly expressed in one or more parts of the silk glands. An enrichment analysis performed using the functional annotation data of the transcripts provided novel insights into the functions of the silk gland parts. Therefore, the reference transcriptome data set obtained has extended B. mori genomic and transcriptomic insights and may contribute to advances in entomologic and vertebrate research, including that on humans.

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Section: Discussionsupporting

confidence: 55%

“…S6). Sericin-4 (Ser4) is another sericin protein that is composed of 34 exons [24]. This gene is split into three distinct structures in GMD (KWMTBOMO06324, KWMTBOMO06325, and KWMTBOMO06326), whereas RTD provided an exact model (MSTRG.2610.1) (Additional file 12: Fig.…”

Section: Elaborated Structures Of Silk Genesmentioning

confidence: 99%

Reference transcriptome data in silkwormBombyx mori

Yokoi

Tsubota

Sun

et al. 2019

Preprint

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“…We speculate that the gene corresponding to 54578_c1_g2_i1 would have some other isoform(s) that is encoding larger protein. Studies in other lepidopteran species have revealed that four sericin genes are present in B. mori, five in Antheraea yamamai and S. ricini, and three in G. mellonella (Okamoto et al, 1982;Takasu et al, 2007;Takasu et al, 2010;Žurovec et al, 2013;Žurovec et al, 2016;Tsubota et al, 2016b;Dong et al, 2019). Thus, the presence of multiple sericin genes in one species appears to be a common feature in Lepidoptera.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of the bagworm moth silk gland reveals a number of silk genes conserved within Lepidoptera

et al. 2020

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Lepidopteran insects produce cocoons with unique properties. The cocoons are made of silk produced in the larval tissue silk gland and our understanding of the silk genes is still very limited. Here, we investigated silk genes in the bagworm moth Eumeta variegata, a species that has recently been found to produce extraordinarily strong and tough silk. Using short-read transcriptomic analysis, we identified a partial sequence of the fibroin heavy chain gene and its product was found to have a C-terminal structure that is conserved within nonsaturniid species. This is in accordance with the presence of fibroin light chain/fibrohexamerin genes and it is suggested that the bagworm moth is producing silk composed of fibroin ternary complex. This indicates that the fibroin structure has been evolutionarily conserved longer than previously thought. Other than fibroins we identified candidates for sericin genes, expressed strongly in the middle region of the silk gland and encoding serine-rich proteins, and other silk genes, that are structurally conserved with other lepidopteran homologues. The bagworm moth is thus considered to be producing conventional lepidopteran type of silk. We further found a number of genes expressed in a specific region of the silk gland and some genes showed conserved expression with Bombyx mori counterparts. This is the first study allowing comprehensive silk gene identification and expression analysis in the lepidopteran Psychidae family and should contribute to the understanding of silk gene evolution as well as to the development of novel types of silk.

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“…In contrast, our RTD (MSTRG.2595.1) successfully provided an accurate gene structure (Figure S6). Sericin-4 (Ser4) is another sericin protein that is composed of 34 exons [36]. This gene is split into three distinct structures in GMD (KWMT-BOMO06324, KWMTBOMO06325, and KWMTBOMO06326), whereas RTD provided an exact model (MSTRG.2610.1; Figure S7).…”

Section: Elaborated Structures Of Silk Genesmentioning

confidence: 99%

Reference Transcriptome Data in Silkworm Bombyx mori

Yokoi

Tsubota

Jouraku

et al. 2021

Insects

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Herein, we performed RNA-seq analysis of ten major tissues/subparts of silkworm larvae. The sequences were mapped onto the reference genome assembly and the reference transcriptome data were successfully constructed. The reference data provided a nearly complete sequence for sericin-1, a major silk gene with a complex structure. We also markedly improved the gene model for other genes. The transcriptomic expression was investigated in each tissue and a number of transcripts were identified that were exclusively expressed in tissues such as the testis. Transcripts strongly expressed in the midgut formed tight genomic clusters, suggesting that they originated from tandem gene duplication. Transcriptional factor genes expressed in specific tissues or the silk gland subparts were also identified. We successfully constructed reference transcriptome data in the silkworm and found that a number of transcripts showed unique expression profiles. These results will facilitate basic studies on the silkworm and accelerate its applications, which will contribute to further advances in lepidopteran and entomological research as well as the practical use of these insects.

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Identification of Bombyx mori sericin 4 protein as a new biological adhesive

Cited by 48 publications

References 28 publications

Reference transcriptome data in silkwormBombyx mori

Reference transcriptome data in silkwormBombyx mori

Transcriptomic analysis of the bagworm moth silk gland reveals a number of silk genes conserved within Lepidoptera

Reference Transcriptome Data in Silkworm Bombyx mori

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