Identification of BOIP, a novel cDNA highly expressed during spermatogenesis that encodes a protein interacting with the orange domain of the hairy‐related transcription factor HRT1/Hey1 in Xenopus and mouse

Abstract

Hairy-related transcription factor (HRT/Hey) genes encode a novel subfamily of basic helix-loop-helix (bHLH) transcription factors related to the Drosophila hairy and Enhancer-of-split (E(spl)) and the mammalian HES proteins that function as downstream mediators of Notch signaling. Using the yeast two-hybrid approach, a previously uncharacterized protein was identified in Xenopus that interacts with XHRT1 (originally referred to as bc8), one member of the HRT/Hey subclass. This protein is evolutionarily conserved in chordates. It binds to sequences adjacent to the bHLH domain of XHRT1 known as the Orange domain and has been named bc8 Orange interacting protein (BOIP). BOIP shows a rather uniform subcellular localization and is recruited to the nucleus upon binding to XHRT1. In Xenopus, XBOIP mRNA is detected by RNase protection analysis throughout embryogenesis. In the adult, the strongest expression is detected in testis. In the mouse, high levels of BOIP mRNA are also found in adult testis. No expression is detected in the embryo and in any of the other adult organs tested. In situ hybridization revealed that BOIP transcripts were detected almost exclusively in round spermatids and that this expression overlaps with that of Hey1 (HRT1), which is expressed throughout spermatogenesis. In view of the importance of the Orange domain for HRT/Hey function, the newly identified BOIP proteins may serve as regulators specifically of HRT1/Hey1 activity. Developmental Dynamics 228:716 -725, 2003.

Hey Basic Helix-Loop-Helix Transcription Factors Are Repressors of GATA4 and GATA6 and Restrict Expression of the GATA Target Gene ANF in Fetal Hearts

“…HEK293T cells were transiently transfected with the indicated expression vectors for FLAG-or HA-tagged proteins. Coimmunoprecipitation was performed as described (54).…”

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

“…Coiled-coil domain containing 89 [ID: T4-852] interacts with the orange domain of the hairy-related transcription factor; mRNA for this protein is expressed in round spermatids (Van Wayenbergh et al, 2003). Transcription factor-like 5 (TCFL5) [ID: T4-853] is highly expressed in primary spermatocytes at the diplotene stage of meiotic prophase where the protein interacts with transcriptionally active chromatin.…”

A deficiency of lunatic fringe is associated with cystic dilation of the rete testis

“…Evidence from studies in worms, flies, and mice predict a role for Notch signaling during folliculogenesis and oocyte maturation (Francis et al 1995, Kadyk & Kimble 1998, Deng et al 2001, Johnson et al 2001, Lopez-Schier & St Johnston 2001, Hansen et al 2004, Hahn et al 2005). Expression of the Notch receptors, their ligands, and downstream target genes has been detected in the neonatal and adult mammalian testes (Dirami et al 2001, van Wayenbergh et al 2003, Hayashi et al 2004, von Schönfeldt et al 2004). It has been demonstrated that Notch1 and Jagged1 (Jag1 ) are expressed in the vasculature of the embryonic testis (Brennan et al 2002).…”