Identification of blood source preferences and Leishmania infection in sand flies (Diptera: Psychodidae) in north-eastern Algeria

Messahel, Nacer Eddine; Benallal, Kamal Eddine; Halada, Petr; Lafri, Ismail; Manseur, Hemza; Hakem, Ahcène; Houali, Karim; Harrat, Z.; Volf, Petr; Dvořák, Vít

doi:10.1016/j.vprsr.2022.100729

Cited by 5 publications

(2 citation statements)

References 49 publications

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“…VL is fatal if left untreated (8). According to Solano-Gallego et al and Messahel et al (2,9) the parasite responsible for Mediterranean foci is Leishmania infantum. In Algeria, Phlebotomus pernicious (Figure 1C) is recognized as the vector of this infection (10).…”

Section: Introductionmentioning

confidence: 99%

Anti-leishmanial activities of Olea europaea subsp. laperrinei extracts

Souad Lahcene,

Lamia Trabelsi,

Mounir M. Salem-Bekhit

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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Section: Introductionmentioning

confidence: 99%

Anti-leishmanial activities of Olea europaea subsp. laperrinei extracts

Souad Lahcene,

Lamia Trabelsi,

Mounir M. Salem-Bekhit

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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“…It has also been successfully applied to the identification of sandflies [22][23][24][25]. It allows the identification of immature stages [26] and blood meals [27,28] in engorged females.…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF MS Limits for the Identification of Mediterranean Sandflies of the Subgenus Larroussius, with a Special Focus on the Phlebotomus perniciosus Complex

et al. 2022

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Leishmania infantum is the agent of visceral leishmaniasis in the Mediterranean basin. It is transmitted by sandflies of the subgenus Larroussius. Although Phlebotomus perniciosus is the most important vector in this area, an atypical Ph. perniciosus easily confused with Ph. longicuspis has been observed in North Africa. MALDI-TOF MS, an important tool for vector identification, has recently been applied for the identification of sandflies. Spectral databases presented in the literature, however, include only a limited number of Larroussius species. Our objective was to create an in-house database to identify Mediterranean sandflies and to evaluate the ability of MALDI-TOF MS to discriminate close species or atypical forms within the Larroussius subgenus. Field-caught specimens (n = 94) were identified morphologically as typical Ph. perniciosus (PN; n = 55), atypical Ph. perniciosus (PNA; n = 9), Ph. longicuspis (n = 9), Ph. ariasi (n = 9), Ph. mascittii (n = 3), Ph. neglectus (n = 5), Ph. perfiliewi (n = 1), Ph. similis (n = 9) and Ph. papatasi (n = 2). Identifications were confirmed by sequencing of the mtDNA CytB region and sixteen specimens were included in the in-house database. Blind assessment on 73 specimens (representing 1073 good quality spectra) showed a good agreement (98.5%) between MALDI-TOF MS and molecular identification. Discrepancies concerned confusions between Ph. perfiliewi and Ph. perniciosus. Hierarchical clustering did not allow classification of PN and PNA. The use of machine learning, however, allowed discernment between PN and PNA and between the lcus and lcx haplotypes of Ph. longicuspis (accuracy: 0.8938 with partial-least-square regression and random forest models). MALDI-TOF MS is a promising tool for the rapid and accurate identification of field-caught sandflies. The use of machine learning could allow to discriminate similar species.

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The complexities of blood‐feeding patterns in mosquitoes and sandflies and the burden of disease: A minireview

Bursali,

Touray

2024

Veterinary Medicine & Sci

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Mosquitoes and sandflies exhibit a wide range of blood feeding patterns, targeting a wide range of vertebrate species, including birds, mammals, reptiles, and amphibians, for proteins vital for egg development. This broad host range increases the opportunity for them to acquire pathogens of numerous debilitating‐and‐fatal diseases from various animal reservoirs, playing a significant role in disease crossover between animals and humans, also known as zoonotic transmission. This review focuses on the intricate blood‐feeding habits of these dipteran vectors, their sensory systems and the complex dance between host and pathogen during disease transmission. We delve into the influence of blood sources on pathogen spread by examining the insect immune response and its intricate interplay with pathogens. The remarkable sense of smell guiding them towards food sources and hosts is explored, highlighting the interplay of multiple sensory cues in their navigation. Finally, we examine the challenges in mosquito control strategies and explore innovations in this field, emphasizing the need for sustainable solutions to combat this global health threat. By understanding the biology and behaviour of these insects, we can develop more effective strategies to protect ourselves and mitigate the burden of vector‐borne diseases.

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Identification of blood source preferences and Leishmania infection in sand flies (Diptera: Psychodidae) in north-eastern Algeria

Cited by 5 publications

References 49 publications

Anti-leishmanial activities of Olea europaea subsp. laperrinei extracts

Anti-leishmanial activities of Olea europaea subsp. laperrinei extracts

MALDI-TOF MS Limits for the Identification of Mediterranean Sandflies of the Subgenus Larroussius, with a Special Focus on the Phlebotomus perniciosus Complex

The complexities of blood‐feeding patterns in mosquitoes and sandflies and the burden of disease: A minireview

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