Identification of blood meal sources in species of genus Rhodnius in four different environments in the Brazilian amazon

Bilheiro, Adriana Benatti; Costa, Glaucilene da Silva; Araújo, Maísa da Silva; Ribeiro, Willian Augusto Rocha; Medeiros, Jansen Fernandes; Camargo, Luís Marcelo Aranha

doi:10.1016/j.actatropica.2022.106486

Cited by 4 publications

(5 citation statements)

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“…The striking difference in the fractions with determined vertebrate hosts is undoubtedly related to the distinct physiology of triatomines and soft ticks. While the two vectors feed on similar hosts, including mammals, reptiles, and birds ( 10 , 37 ), soft ticks are known to endure extremely long starvation periods ( 38 ) that may hamper the molecular detection of ingested blood meal ( 39 ). For T. protracta (38/39), T. rubida (34/46), and T. lecticularia (10/12), packrats ( Neotoma sp.)…”

Section: Resultsmentioning

confidence: 99%

“…While the majority of the approximately 7,000 Reduviidae species are comprised of hemolymphophagous assassin bugs preying on other invertebrates, the subfamily Triatominae, commonly known as kissing bugs and the vectors of Trypanosoma cruzi , converted to hematophagy on birds, mammals, and reptiles ( 9 , 10 ). In some respects, the symbiosis of Triatominae (Reduviidae) resembles that of hematophagous vertebrates.…”

Section: Introductionmentioning

confidence: 99%

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Microbiomes of Blood-Feeding Triatomines in the Context of Their Predatory Relatives and the Environment

et al. 2023

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This study places microbiomes of blood-feeding North American Triatoma vectors (Reduviidae) into a broader evolutionary and ecological context provided by related predatory assassin bugs (Reduviidae), another unrelated vector species (soft tick Ornithodoros turicata ), and the environment these arthropods coinhabit. For both vectors, microbiome analyses suggest three interrelated sources of bacteria, i.e., the microbiome of vertebrate nests as their natural habitat, the vertebrate skin microbiome, and the pathobiome circulating in vertebrate blood.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbiomes of Blood-Feeding Triatomines in the Context of Their Predatory Relatives and the Environment

et al. 2023

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“…The striking difference in the fractions with determined vertebrate host is undoubtedly related to the distinct physiology of triatomines and soft ticks. While both vectors feed on similar hosts, including mammals, reptiles and birds (10, 60), soft ticks are known to endure extremely long starvation periods (61) that may hamper the molecular detection of ingested blood meal (62). For T. protracta (38/39), T. rubida (34/46) and T. lecticularia (10/12), packrats, Neotoma sp., were indeed the most common blood meal (82/93, 88%).…”

Section: Resultsmentioning

confidence: 99%

“…While the majority of the approximately 7000 Reduviidae species are comprised of hemolymphophagous assassin bugs preying on other invertebrates, the subfamily Triatominae, commonly known as kissing bugs and the vectors of Trypanosama cruzi , converted to hematophagy on birds, mammals and reptiles (9, 10). In some respect, symbiosis of Triatominae (Reduviidae), resembles that of hematophagous vertebrates.…”

Section: Introductionmentioning

confidence: 99%

Microbiomes of blood feeding triatomines in the context of their predatory relatives and the environment

Tarabai

Floriano

Zima

et al. 2023

Preprint

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The importance of gut microbiomes has become generally recognized in vector biology. This study addresses microbiome signatures in North American Triatoma species of public health significance (vectors of Trypanosoma cruzi) linked to their blood feeding strategy and the natural habitat. To place the Triatoma associated microbiomes within a complex evolutionary and ecological context, we sampled sympatric Triatoma populations, related predatory reduviids, unrelated ticks, and environmental material from vertebrate nests where these arthropods reside. Along with five Triatoma species, we have characterized microbiomes of five reduviids (Stenolemoides arizonensis, Ploiaria hirticornis, Zelus longipes, and two Reduvius species), a single soft tick species, Ornithodoros turicata, and environmental microbiomes from selected sites in Arizona, Texas, Florida and Georgia. The microbiomes of predatory reduviids lack a shared core microbiota. Like in triatomines, microbiome dissimilarities among species corelate with dominance of a single bacterial taxa. These include Rickettsia, Lactobacillus, Candidatus Midichloria, and Zymobacter, which are often accompanied by known symbiotic genera, i.e., Wolbachia, Candidatus Lariskella, Asaia, Gilliamella, and Burkholderia. We have further identified compositional convergence of analyzed microbiomes in respect to the host phylogenetic distance in both blood feeding and predatory reduviids. While microbiomes of two reduviid species from Emesinae family reflect their close relationship, the microbiomes of all Triatoma species repeatedly form a distinct monophyletic cluster highlighting their phylosymbiosis. Furthermore, based on environmental microbiome profiles and blood meal analysis, we propose three epidemiologically relevant and mutually interrelated bacterial sources for Triatoma microbiomes, i.e., host abiotic environment, host skin microbiome, and pathogens circulating in host blood.

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“…These findings showed that, regarding the NTD elimination programs focused on vector control and diagnosis/treatment in humans, the pathogen transmission remains enzootic [77]. The above is alarming considering the increasing sylvatic niche fragmentation, which also increases the risk of outbreaks, sylvatic parasite species circulation in urban transmission cycles, and the adaptation of the pathogens according to the availability of vectors and hosts [78,79]. This problem has been acknowledged and studied in endemic regions in the Brazilian Amazon, keeping in mind their local context and associated variables to strengthen One Health intervention programs [80].…”

Section: Discussionmentioning

confidence: 99%

An overview of the trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites infecting several mammal species in Colombia

et al. 2022

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Background Trypanosomatids are among the most critical parasites for public health due to their impact on human, animal, and plant health. Diseases associated with these pathogens manifest mainly in poor and vulnerable populations, where social, environmental, and biological factors modulate the case incidence and geographical distribution. Methods We used Sanger and amplicon-based next-generation sequencing (NGS) in samples from different mammals to identify trypanosomatid infections in several departments in Colombia. A total of 174 DNA samples (18 humans, 83 dogs, and 73 wild mammals) were analyzed by conventional PCR using a fragment of the heat shock protein 70 (Hsp70) gene and Sanger sequenced the positive samples. Twenty-seven samples were sent for amplicon-based NGS using the same gene fragment. Data obtained were used to perform diversity analyses. Results One hundred and thirteen samples were positive for PCR by Hsp70 fragment; these corresponded to 22.1% Leishmania spp., 18.6% L. amazonensis, 9.7% L. braziliensis, 14.2% L. infantum, 8% L. panamensis, and 27.4% Trypanosoma cruzi. Comparison of the identified species by the two sequencing technologies used resulted in 97% concordance. Alpha and beta diversity indices were significant, mainly for dogs; there was an interesting index of coinfection events in the analyzed samples: different Leishmania species and the simultaneous presence of T. cruzi and even T. rangeli in one of the samples analyzed. Moreover, a low presence of L. braziliensis was observed in samples from wild mammals. Interestingly, to our knowledge, this is the first report of Leishmania detection in Hydrochaeris hydrochaeris (capybara) in Colombia. Conclusions The Hsp70 fragment used in this study is an optimal molecular marker for trypanosomatid identification in many hosts and allows the identification of different species in the same sample when amplicon-based sequencing is used. However, the use of this fragment for molecular diagnosis through conventional PCR should be carefully interpreted because of this same capacity to identify several parasites. This point is of pivotal importance in highly endemic countries across South America because of the co-circulation of different genera from the Trypanosomatidae family. The findings show an interesting starting point for One Health approaches in which coevolution and vector-host interactions can be studied. Graphical Abstract

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Identification of blood meal sources in species of genus Rhodnius in four different environments in the Brazilian amazon

Cited by 4 publications

References 58 publications

Microbiomes of Blood-Feeding Triatomines in the Context of Their Predatory Relatives and the Environment

Microbiomes of Blood-Feeding Triatomines in the Context of Their Predatory Relatives and the Environment

Microbiomes of blood feeding triatomines in the context of their predatory relatives and the environment

An overview of the trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites infecting several mammal species in Colombia

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