Identification of black pine (<i>Pinus nigra</i>Arn.) heartwood as a rich source of bioactive stilbenes by qNMR

Ioannidis, Kostas; Melliou, Eleni; Alizoti, Paraskevi; Magiatis, Prokopios

doi:10.1002/jsfa.8090

Cited by 22 publications

(33 citation statements)

References 45 publications

(81 reference statements)

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“…Reduced decay rate of dead wood decreases release of CO 2 back to the atmosphere. Concentrations of both resin acids ( Karppanen et al, 2007 ) and stilbenes ( Ioannidis et al, 2017 ) in heartwood of Pinus sp. can exceed 10% of dry weight and significantly reduce decay rate of dead wood by fungal rot ( Karppanen et al, 2007 ) and by woodborers ( Nerg et al, 2004 ).…”

Section: Psc Transmitted Ecosystem Feedbacks To Climate Changementioning

confidence: 99%

Climate Change Effects on Secondary Compounds of Forest Trees in the Northern Hemisphere

et al. 2018

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Plant secondary compounds (PSCs), also called secondary metabolites, have high chemical and structural diversity and appear as non-volatile or volatile compounds. These compounds may have evolved to have specific physiological and ecological functions in the adaptation of plants to their growth environment. PSCs are produced by several metabolic pathways and many PSCs are specific for a few plant genera or families. In forest ecosystems, full-grown trees constitute the majority of plant biomass and are thus capable of producing significant amounts of PSCs. We summarize older literature and review recent progress in understanding the effects of abiotic and biotic factors on PSC production of forest trees and PSC behavior in forest ecosystems. The roles of different PSCs under stress and their important role in protecting plants against abiotic and biotic factors are also discussed. There was strong evidence that major climate change factors, CO2 and warming, have contradictory effects on the main PSC groups. CO2 increases phenolic compounds in foliage, but limits terpenoids in foliage and emissions. Warming decreases phenolic compounds in foliage but increases terpenoids in foliage and emissions. Other abiotic stresses have more variable effects. PSCs may help trees to adapt to a changing climate and to pressure from current and invasive pests and pathogens. Indirect adaptation comes via the effects of PSCs on soil chemistry and nutrient cycling, the formation of cloud condensation nuclei from tree volatiles and by CO2 sequestration into PSCs in the wood of living and dead forest trees.

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Section: Psc Transmitted Ecosystem Feedbacks To Climate Changementioning

confidence: 99%

Climate Change Effects on Secondary Compounds of Forest Trees in the Northern Hemisphere

et al. 2018

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“…As it was previously shown in the cases of olive oil [43,44], beer [45], wine [33], and stilbenes from Pinus nigra heartwood [3], qNMR is the most appropriate method for quantitation. The developed methodology for the analysis of heartwood resin acids requires less than a minute to record the spectrum and perform the analytes quantitation, whereas using the previously reported 13 C-NMR methodology requires a considerably longer time, i.e., approximately two hours [40] or even more [46].…”

Section: Discussionmentioning

confidence: 99%

“…Venäläinen et al [48] also reported high variation among trees in the resin acid concentrations of Pinus sylvestris . Heartwood resin acid contents present provenance variation as well (Table 5) because of the heterogeneity among populations [3]. In general, the southeastern provenance of Parnonas, grown at the most xerothermic environment [49] and subjected to the strongest winds [50], showed higher values of total resin acids, i.e., for neoabietic acid, palustric acid, and sandaracopimaric acid, while the southwestern of Taigetos had the lowest concentrations of all except for dehydroabietic and levopimaric acids.…”

Section: Discussionmentioning

confidence: 99%

“…Its significance lead to its planting outside its natural range, e.g., North and South America, Australia, and New Zealand. Furthermore, its importance is verified by its potential use for the production of high added value products, i.e., bioactive compounds produced from wood and wood waste materials [2,3]. Such bioactive substances are, inter alia, the resin acids occurring in pine’s oleoresin.…”

Section: Introductionmentioning

confidence: 99%

“…pallasiana marginal provenances from the Peloponnese, southern Greece, growing in a clonal seed orchard. The heartwood of these trees produced an exceptionally high extractive content, approximately 30% w / w [3], making it particularly interesting for the present analysis. The target compounds were the abietane-type resin acids: abietic, dehydroabietic, neoabietic, palustric, and levopimaric acids, and the pimarane-type: pimaric, isopimaric, and sandaracopimaric acids.…”

Section: Introductionmentioning

confidence: 99%

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High-Throughput 1H-Nuclear Magnetic Resonance-Based Screening for the Identification and Quantification of Heartwood Diterpenic Acids in Four Black Pine (Pinus nigra Arn.) Marginal Provenances in Greece

Ioannidis¹,

Melliou

Magiatis

2019

Molecules

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A high-throughput quantitative Nuclear Magnetic Resonance 1H-NMR method was developed and applied to screen the quantity of the diterpenic resin acids in the heartwood of black pine, due to the renewed scientific interest in their medicinal properties and use in various diseases treatment. The 260 samples were taken from Pinus nigra clones, selected from four provenances of the Peloponnese (Greece), participating in a 35-year-old clonal seed orchard. Total resin acids per dry heartwood weight (dhw) varied greatly, ranging from 30.05 to 424.70 mg/gdhw (average 219.98 mg/gdhw). Abietic was the predominant acid (76.77 mg/gdhw), followed by palustric acid (47.94 mg/gdhw), neoabietic acid (39.34 mg/gdhw), and pimaric acid (22.54 mg/gdhw). Dehydroabietic acid was at moderate levels (11.69 mg/gdhw), while levopimaric, isopimaric, and sandaracopimaric acids were in lower concentrations. The resin acid fraction accounted for 72.33% of the total acetone extractives. Stilbenes were presented in significant quantities (19.70%). The resin acid content was composed mainly of the abietane type resin acids (83.56%). Peloponnesian Pinus nigra heartwood was found to be the richest source of resin acids identified to date and is considered the best natural source for the production of such bioactive extracts. The results indicate a high potential for effective selection and advanced breeding of pharmaceutical and high economic value bioactive substances from Pinus nigra clones.

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Conservation and Breeding of Mediterranean Pines

Climent¹,

Alizoti

Rodríguez-Quilón³

et al. 2021

Pines and Their Mixed Forest Ecosystems in the Mediterranean Basin

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Identification of black pine (Pinus nigraArn.) heartwood as a rich source of bioactive stilbenes by qNMR

Abstract: Pinus nigra heartwood is the richest source of pinosylvin and pinosylvin monomethyl ether identified to date and can be considered the best natural resource for production of bioactive extracts. © 2016 Society of Chemical Industry.

Cited by 22 publications

References 45 publications

Climate Change Effects on Secondary Compounds of Forest Trees in the Northern Hemisphere

Climate Change Effects on Secondary Compounds of Forest Trees in the Northern Hemisphere

High-Throughput 1H-Nuclear Magnetic Resonance-Based Screening for the Identification and Quantification of Heartwood Diterpenic Acids in Four Black Pine (Pinus nigra Arn.) Marginal Provenances in Greece

Conservation and Breeding of Mediterranean Pines

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