Identification of bHLH genes through genome-wide association study and antisense expression of ZjbHLH076/ZjICE1 influence tolerance to low temperature and salinity in Zoysia japonica

Zuo, Zhi-Fang; Sun, Hui; Lee, Hyo-Yeon; Kang, Hong‐Gyu

doi:10.1016/j.plantsci.2021.111088

Cited by 14 publications

(14 citation statements)

References 71 publications

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“…These results indicated that CibHLH96 also participated in regulating citrus flowering. Many bHLH transcription factors have been reported to be involved in abiotic stresses, such as drought and low temperature (Li et al, 2019; Zuo et al, 2021). So the expression of CibHLH96 was also investigated in the drought‐treated adult kumquat and low temperature‐treated adult sweet orange.…”

Section: Resultsmentioning

confidence: 99%

A bZIP transcription factor (CiFD) regulates drought‐ and low‐temperature‐induced flowering by alternative splicing in citrus

Zhang

et al. 2022

JIPB

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Drought and low temperature are two key environmental factors that induce adult citrus flowering. However, the underlying regulation mechanism is poorly understood. The bZIP transcription factor FD is a key component of the florigen activation complex (FAC) which is composed of FLOWERING LOCUS T (FT), FD, and 14-3-3 proteins. In this study, isolation and characterization of CiFD in citrus found that there was alternative splicing (AS) of CiFD, forming two different proteins (CiFDα and CiFDβ). Further investigation found that their expression patterns were similar in different tissues of citrus, but the subcellular localization and transcriptional activity were different. Overexpression of the CiFD DNA sequence (CiFD-DNA), CiFDα, or CiFDβ in tobacco and citrus showed early flowering, and CiFD-DNA transgenic plants were the earliest, followed by CiFDβ and CiFDα. Interestingly, CiFDα and CiFDβ were induced by low temperature and drought, respectively. Further analysis showed that CiFDα can form a FAC complex with CiFT, Ci14-3-3, and then bind to the citrus APETALA1 (CiAP1) promoter and promote its expression. However, CiFDβ can directly bind to the CiAP1 promoter independently of CiFT and Ci14-3-3. These results showed that CiFDβ can form a more direct and simplified pathway that is independent of the FAC complex to regulate drought-induced flowering through AS. In addition, a bHLH transcription factor (CibHLH96) binds to CiFD promoter and promotes the expression of CiFD under drought condition. Transgenic analysis found that CibHLH96 can promote flowering in transgenic tobacco. These results suggest that CiFD is involved in drought-and low-temperature-induced citrus flowering through different regulatory patterns.

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Section: Resultsmentioning

confidence: 99%

A bZIP transcription factor (CiFD) regulates drought‐ and low‐temperature‐induced flowering by alternative splicing in citrus

Zhang

et al. 2022

JIPB

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“…The overexpression of MxbHLH18 in apple increased iron and high-salinity stress tolerances in Arabidopsis [ 140 ]. Zuo et al [ 34 ] reported the genome-wide identification of the bHLH family genes in Zoysia japonica . The expressions of ZjbHLH62/ZjICE2 , ZjbHLH67 , ZjbHLH76/ZjICE1 , ZjbHLH88 , ZjbHLH97 , and ZjbHLH120 in subfamily IIIb were affected by cold, salt, dehydration, and/or ABA [ 34 ].…”

Section: The Bhlh Transcription Factor Family In Plantsmentioning

confidence: 99%

“…Zuo et al [ 34 ] reported the genome-wide identification of the bHLH family genes in Zoysia japonica . The expressions of ZjbHLH62/ZjICE2 , ZjbHLH67 , ZjbHLH76/ZjICE1 , ZjbHLH88 , ZjbHLH97 , and ZjbHLH120 in subfamily IIIb were affected by cold, salt, dehydration, and/or ABA [ 34 ]. Furthermore, the overexpression of ZjICE1 and ZjICE2 endowed transgenic Arabidopsis and Z. japonica plants with abiotic stress tolerance via the activation of the DREB/CBF regulon, and they also enhanced ROS scavenging [ 141 , 142 ].…”

Section: The Bhlh Transcription Factor Family In Plantsmentioning

confidence: 99%

“… Phylogenetic analysis of the species based on genome-wide association study of the basic helix-loop-helix (bHLH) family. The total number of bHLH transcription factors identified from the genome of each species are indicated [ 5 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ]. …”

Section: Figurementioning

confidence: 99%

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Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

Zuo

Lee

Kang

2023

IJMS

Self Cite

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Plant basic helix-loop-helix (bHLH) transcription factors are involved in many physiological processes, and they play important roles in the abiotic stress responses. The literature related to genome sequences has increased, with genome-wide studies on the bHLH transcription factors in plants. Researchers have detailed the functionally characterized bHLH transcription factors from different aspects in the model plant Arabidopsis thaliana, such as iron homeostasis and abiotic stresses; however, other important economic crops, such as rice, have not been summarized and highlighted. The bHLH members in the same subfamily have similar functions; therefore, unraveling their regulatory mechanisms will help us to identify and understand the roles of some of the unknown bHLH transcription factors in the same subfamily. In this review, we summarize the available knowledge on functionally characterized bHLH transcription factors according to four categories: plant growth and development; metabolism synthesis; plant signaling, and abiotic stress responses. We also highlight the roles of the bHLH transcription factors in some economic crops, especially in rice, and discuss future research directions for possible genetic applications in crop breeding.

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“…Anthocyanins may scavenge ROS as an efficient antioxidant by neutralizing radicals with their hydroxyl groups to preserve normal cellular redox homeostasis; hence, plants often generate anthocyanins to reduce oxidative damage caused by low-temperature stress [ 20 , 21 , 22 , 23 , 24 , 25 ]. Low-temperature responsive networks in zoysiagrass have been investigated through physiological, biochemical, molecular, genetic and omic approaches [ 7 , 15 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. However, no studies have attempted to associate anthocyanin accumulation with cold tolerance in zoysiagrass.…”

Section: Introductionmentioning

confidence: 99%

A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype

Jin

Jiang

Yang

et al. 2022

Plants

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Zoysia japonica is a warm-season turfgrass that is extensively used in landscaping, sports fields, and golf courses worldwide. Uncovering the low-temperature response mechanism of Z. japonica can help to accelerate the development of new cold-tolerant cultivars, which could be used to prolong the ornamental and usage duration of turf. A novel Z. japonica biotype, YueNong-9 (YN-9), was collected from northeastern China for this study. Phenotypic measurements, cold-tolerance investigation, and whole-transcriptome surveys were performed on YN-9 and LanYin-3 (LY-3), the most popular Z. japonica cultivar in Southern China. The results indicated the following: YN-9 has longer second and third leaves than LY-3; when exposed to the natural low temperature during winter in Guangzhou, YN-9 accumulated 4.74 times more anthocyanin than LY-3; after cold acclimation and freezing treatment, 83.25 ± 9.55% of YN-9 survived while all LY-3 leaves died, and the dark green color index (DGCI) value of YN-9 was 1.78 times that of LY-3; in YN-9, there was a unique up-regulation of Phenylalanine ammonia-lyase (PAL), Homeobox-leucine Zipper IV (HD-ZIP), and ATP-Binding Cassette transporter B8 (ABCB8) expressions, as well as a unique down-regulation of zinc-regulated transporters and iron-regulated transporter-like proteins (ZIPs) expression, which may promote anthocyanin biosynthesis, transport, and accumulation. In conclusion, YN-9 exhibited enhanced cold tolerance and is thus an excellent candidate for breeding cold-tolerant Z. japonica variety, and its unique low-temperature-induced anthocyanin accumulation and gene responses provide ideas and candidate genes for the study of low-temperature tolerance mechanisms and genetic engineering breeding.

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Identification of bHLH genes through genome-wide association study and antisense expression of ZjbHLH076/ZjICE1 influence tolerance to low temperature and salinity in Zoysia japonica

Cited by 14 publications

References 71 publications

A bZIP transcription factor (CiFD) regulates drought‐ and low‐temperature‐induced flowering by alternative splicing in citrus

A bZIP transcription factor (CiFD) regulates drought‐ and low‐temperature‐induced flowering by alternative splicing in citrus

Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype

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