Identification of beer-spoilage bacteria using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Wieme, Anneleen D.; Spitaels, Freek; Aerts, Maarten; Bruyne, Katrien De; Landschoot, Anita Van; Vandamme, Peter

doi:10.1016/j.ijfoodmicro.2014.05.003

Cited by 64 publications

(41 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, there are a lot of approaches to apply this method for the classification of food fermentation microbiota and starter cultures [49, 50], food spoilage microbiota [51] or beverage spoiling strains [31, 52]. This method demonstrates the potential to investigate the influences of different stress responses on microorganisms in different media composition.…”

Section: Discussionmentioning

confidence: 99%

MALDI-TOF MS typing enables the classification of brewing yeasts of the genus Saccharomyces to major beer styles

et al. 2017

View full text Add to dashboard Cite

Brewing yeasts of the genus Saccharomyces are either available from yeast distributor centers or from breweries employing their own "in-house strains". During the last years, the classification and characterization of yeasts of the genus Saccharomyces was achieved by using biochemical and DNA-based methods. The current lack of fast, cost-effective and simple methods to classify brewing yeasts to a beer type, may be closed by Matrix Assisted Laser Desorption/Ionization-Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) upon establishment of a database based on sub-proteome spectra from reference strains of brewing yeasts. In this study an extendable "brewing yeast" spectra database was established including 52 brewing yeast strains of the most important types of bottom-and top-fermenting strains as well as beer-spoiling S. cerevisiae var. diastaticus strains. 1560 single spectra, prepared with a standardized sample preparation method, were finally compared against the established database and investigated by bioinformatic analyses for similarities and distinctions. A 100% separation between bottom-, top-fermenting and S. cerevisiae var. diastaticus strains was achieved. Differentiation between Alt and Kö lsch strains was not achieved because of the high similarity of their protein patterns. Whereas the Ale strains show a high degree of dissimilarity with regard to their sub-proteome. These results were supported by MDS and DAPC analysis of all recorded spectra. Within five clusters of beer types that were distinguished, and the wheat beer (WB) cluster has a clear separation from other groups. With the establishment of this MALDI-TOF MS spectra database proof of concept is provided of the discriminatory power of this technique to classify brewing yeasts into different major beer types in a rapid, easy way, and focus brewing trails accordingly. It can be extended to yeasts for specialty beer types and other applications including wine making or baking.

show abstract

Section: Discussionmentioning

confidence: 99%

MALDI-TOF MS typing enables the classification of brewing yeasts of the genus Saccharomyces to major beer styles

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Some AAB are also of interest to the industry because of their capacity to produce cellulose or other chemicals, such as L-sorbose involved in the synthesis of vitamin C [4]. Furthermore, AAB occur as plant growth-promoting bacteria [5], insect endosymbionts [6], and as spoilers of many kinds of beverages such as wine and beer [7]. AAB are classified in the family Acetobacteraceae within the Alphaproteobacteria .…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Sequence Analysis of Bombella intestini LMG 28161T, a Novel Acetic Acid Bacterium Isolated from the Crop of a Red-Tailed Bumble Bee, Bombus lapidarius

Illeghems

Kerrebroeck

et al. 2016

PLoS ONE

Self Cite

View full text Add to dashboard Cite

The whole-genome sequence of Bombella intestini LMG 28161T, an endosymbiotic acetic acid bacterium (AAB) occurring in bumble bees, was determined to investigate the molecular mechanisms underlying its metabolic capabilities. The draft genome sequence of B. intestini LMG 28161T was 2.02 Mb. Metabolic carbohydrate pathways were in agreement with the metabolite analyses of fermentation experiments and revealed its oxidative capacity towards sucrose, D-glucose, D-fructose and D-mannitol, but not ethanol and glycerol. The results of the fermentation experiments also demonstrated that the lack of effective aeration in small-scale carbohydrate consumption experiments may be responsible for the lack of reproducibility of such results in taxonomic studies of AAB. Finally, compared to the genome sequences of its nearest phylogenetic neighbor and of three other insect associated AAB strains, the B. intestini LMG 28161T genome lost 69 orthologs and included 89 unique genes. Although many of the latter were hypothetical they also included several type IV secretion system proteins, amino acid transporter/permeases and membrane proteins which might play a role in the interaction with the bumble bee host.

show abstract

“…Among recent approaches, the use of protein profiles obtained by MALDI-TOF MS from intact bacteria was successfully applied to differentiate acetic acid bacteria among different genera, species and strains of the same species (Andrés-Barrao, 2012;Andrés-Barrao et al, 2013;Wieme et al, 2014aWieme et al, , 2014b.MALDI-TOF MS was developed byKaras et al (1985Karas et al ( , 1987 andTanaka et al (1988).The basic MALDI-TOF MS instrument makes use of three principal components: (a) a specimen ionization chamber, where the laser-based vaporization of the specimen takes place, (b) a time of flight mass analyzer that separates ions according to their mass to charge ratio (m/z) and (c) a particle detector to monitor separated ions(Dekker and Branda, 2011; Tonolla et al, 2009). Briefly, microbial samples are deposited on a conductive metal plate coated with a chemical matrix .…”

mentioning

confidence: 99%

Updates on quick identification of acetic acid bacteria with a focus on the 16S–23S rRNA gene internal transcribed spacer and the analysis of cell proteins by MALDI-TOF mass spectrometry

Trček

Barja

2015

International Journal of Food Microbiology

View full text Add to dashboard Cite

Identification of beer-spoilage bacteria using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Cited by 64 publications

References 64 publications

MALDI-TOF MS typing enables the classification of brewing yeasts of the genus Saccharomyces to major beer styles

MALDI-TOF MS typing enables the classification of brewing yeasts of the genus Saccharomyces to major beer styles

Whole-Genome Sequence Analysis of Bombella intestini LMG 28161T, a Novel Acetic Acid Bacterium Isolated from the Crop of a Red-Tailed Bumble Bee, Bombus lapidarius

Updates on quick identification of acetic acid bacteria with a focus on the 16S–23S rRNA gene internal transcribed spacer and the analysis of cell proteins by MALDI-TOF mass spectrometry

Contact Info

Product

Resources

About