Identification of bacteriochlorophylls in lakes via reverse‐phase HPLC

Hurley, James P.; Watras, Carl J.

doi:10.4319/lo.1991.36.2.0307

Cited by 51 publications

(28 citation statements)

References 15 publications

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“…Peaks were identified by their absorption and characteristic MH ' and MS 2 spectra and their elution order by comparing with literature data (e.g., Rozema et al 2002;Whitehead & Hedges 2002;Yuan et al 2009 and from Sigma Aldrich (chl a and b, b,o-and b,bcarotene). Concentrations of those molecules for which no standard was available were estimated from peak area, assuming specific extinction coefficients for each compound obtained from the literature (Hurley & Watras 1991;Villanueva et al 1994;Jeffrey et al 1997). Where extinction coefficients were not reported or where no structurally or spectrally similar pigments, the coefficient of b-carotene was used (Jeffrey et al 1997).…”

Section: Methodsmentioning

confidence: 99%

Algal biomass and pigments along a latitudinal gradient in Victoria Land lakes, East Antarctica

Borghini

Colacevich

Caruso³

et al. 2016

Polar Research

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It is generally accepted that Antarctic terrestrial diversity decreases as latitude increases, but latitudinal patterns of several organisms are not always as clear as expected. The Victoria Land region is rich in lakes and ponds and spans 8 degrees of latitude that encompasses gradients in factors such as solar radiation, temperature, ice cover and day length. An understanding of the links between latitudinally driven environmental and biodiversity changes is essential to the understanding of the ecology and evolution of Antarctic biota and the formulation of hypotheses about likely future changes in biodiversity. As several studies have demonstrated that photosynthetic pigments are an excellent, although underused, tool for the study of lacustrine algal communities, the aim of the present study was to investigate variations in algal biomass and biodiversity across the latitudinal gradient of Victoria Land using sedimentary pigments. We test the hypothesis that the biodiversity of freshwater environments decreases as latitude increases. On the basis of our results, we propose using the number of sedimentary pigments as a proxy for algal diversity and the sum of chlorophyll a and bacteriochlorophyll a with their degradation derivatives as an index of biomass. Overall, our data show that biomass and diversity decrease as latitude increases but local environmental conditions, in particular, natural levels of eutrophy, can affect both productivity and diversity

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Section: Methodsmentioning

confidence: 99%

Algal biomass and pigments along a latitudinal gradient in Victoria Land lakes, East Antarctica

Borghini

Colacevich

Caruso³

et al. 2016

Polar Research

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“…Pigments were identified and quantified using standards from Sigma Chemical: chlorophylls a (Chl a) and b, b,b-carotene; and DHI Water and Environment: chlorophyll c2, diadinoxanthin, echinenone, fucoxanthin, lutein, and zeaxanthin. For bacteriochlorophyll e (BChl e), isorenieratene, and bisorenieratene, identification and quantification was done by comparison with published spectra, retention times, and extinction coefficients (Hurley and Watras 1991;Borrego et al 1999). Concentrations of unidentified carotenoids were calculated using the extinction coefficient for b,b-carotene, while those of chlorophyll degradation products were calculated using our calibration coefficients for their respective undegraded chlorophylls.…”

Section: Methodsmentioning

confidence: 99%

Cultural eutrophication, anoxia, and ecosystem recovery in Meretta Lake, High Arctic Canada

Antoniades

Michelutti

Quinlan

et al. 2011

Limnology & Oceanography

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We studied the effects of four decades of cultural eutrophication on Meretta Lake, in the Canadian High Arctic, through a multiproxy analysis of its sediments, including sedimentary pigments, metal concentrations, stable isotope ratios, chironomids, and diatoms. While Meretta Lake's biota clearly responded to nutrient inputs, the manner in which the changes differed from those expected in temperate lakes underlined the profound effects in Arctic lakes of extended ice and snow cover on light penetration, mixing, and interactions with the atmosphere. Hypolimnetic anoxia developed rapidly in Meretta Lake in response to sewage enrichment and was accompanied by the appearance of photosynthetic sulfur bacteria. Benthic communities responded rapidly to sewage inputs, but phytoplankton biomass did not increase until eutrophication was accompanied by climate warming, further reinforcing the importance of ice cover in controlling biotic processes in high Arctic lakes. With climate-mediated ice cover reductions in Meretta Lake, the response to eutrophication began to more closely resemble temperate processes. Recent trajectories indicate that slightly more than a decade after the cessation of sewage inputs, Meretta Lake is recovering toward pre-enrichment conditions.

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“…In stratified lakes having anoxic hypolimnia, photoautotrophic bacteria have been known to contribute substantially to total primary production and biomass (Biebl and Pfennig, 1979;Hurley and Watras, 1991). These bacteria use sulfide and other reduced sulfur compounds and sometimes also iron sulfide as electron donors and light as energy source for anoxygenic photosynthesis (Garcia-Gil et al, 1990;Imhoff, 1992).…”

Section: Introductionmentioning

confidence: 99%

Seasonal occurrence of anoxygenic photosynthesis in Tillari and Selaulim reservoirs, Western India

et al. 2012

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Abstract. Phytoplankton and bacterial pigment compositions were determined by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) in two freshwater reservoirs (Tillari Dam and Selaulim Dam), which are located at the foothills of the Western Ghats in India. These reservoirs experience anoxia in the hypolimnion during summer. Water samples were collected from both reservoirs during anoxic periods while one of them (Tillari Reservoir) was also sampled in winter, when convective mixing results in well-oxygenated conditions throughout the water column. During the period of anoxia (summer), bacteriochlorophyll (BChl) e isomers and isorenieratene, characteristic of brown sulfur bacteria, were dominant in the anoxic (sulfidic) layer of the Tillari Reservoir under low light intensities. The winter observations showed the dominance of small cells of Chlorophyll b-containing green algae and cyanobacteria, with minor presence of fucoxanthin-containing diatoms and peridinin-containing dinoflagellates. Using total BChl e concentration observed in June, the standing stock of brown sulfur bacteria carbon in the anoxic compartment of Tillari Reservoir was estimated to be 2.27 gC m−2, which is much higher than the similar estimate for carbon derived from oxygenic photosynthesis (0.82 gC m−2. The Selaulim Reservoir also displayed similar characteristics with the presence of BChl e isomers and isorenieratene in the anoxic hypolimnion during summer. Although sulfidic conditions prevailed in the water column below the thermocline, the occurrence of photo-autotrophic bacteria was restricted only to mid-depths (maximal concentration of BChl e isomers was detected at 0.2% of the surface incident light). This shows that the vertical distribution of photo-autotrophic sulfur bacteria is primarily controlled by light penetration in the water column where the presence of H2S provides a suitable biogeochemical environment for them to flourish.

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Identification of bacteriochlorophylls in lakes via reverse‐phase HPLC

Cited by 51 publications

References 15 publications

Algal biomass and pigments along a latitudinal gradient in Victoria Land lakes, East Antarctica

Algal biomass and pigments along a latitudinal gradient in Victoria Land lakes, East Antarctica

Cultural eutrophication, anoxia, and ecosystem recovery in Meretta Lake, High Arctic Canada

Seasonal occurrence of anoxygenic photosynthesis in Tillari and Selaulim reservoirs, Western India

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