Identification of Bacterial Species in the Hemolymph of Queen<i>Solenopsis invicta</i>(Hymenoptera: Formicidae)

Tufts, Danielle M.; Bextine, Blake

doi:10.1603/022.038.0502

Cited by 11 publications

(10 citation statements)

References 15 publications

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“…Overall, ants harbored heritable symbionts from six, and possibly eight, lineages of heritable symbionts ( Arsenophonus , Blochmannia , Spiroplasma , and Wolbachia —shown here; Cardinium hertigii and Serratia symbiotica —shown elsewhere [53], [54]; and two candidate symbionts from fire ants [55]; see Figures 4 and S1). The presence of bacteria clustering within clades of other heritable bacteria from the Enterobacteriaceae (Figure S2) suggests that this number could be even higher.…”

Section: Resultsmentioning

confidence: 96%

“…While we estimate six species of heritable symbionts across the ants, this number may be higher. For instance, fire ant queens harbor bacteria in their hemolymph that are also found in eggs and ovaries [55], while other ants harbor bacteria from a symbiotic clade within the Gammaproteobacteria (Figure S2). More work is needed to elucidate the lifestyles of these bacteria, to explore the possibility of non-canonical or rare heritable symbionts across ants and lepidopterans, and to confirm the lifestyles of Arsenophonus and Spiroplasma , which come from lineages where heritability is not ubiquitous [81].…”

Section: Discussionmentioning

confidence: 99%

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A Veritable Menagerie of Heritable Bacteria from Ants, Butterflies, and Beyond: Broad Molecular Surveys and a Systematic Review

et al. 2012

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Maternally transmitted bacteria have been important players in the evolution of insects and other arthropods, affecting their nutrition, defense, development, and reproduction. Wolbachia are the best studied among these and typically the most prevalent. While several other bacteria have independently evolved a heritable lifestyle, less is known about their host ranges. Moreover, most groups of insects have not had their heritable microflora systematically surveyed across a broad range of their taxonomic diversity. To help remedy these shortcomings we used diagnostic PCR to screen for five groups of heritable symbionts—Arsenophonus spp., Cardinium hertigii, Hamiltonella defensa, Spiroplasma spp., and Wolbachia spp.—across the ants and lepidopterans (focusing, in the latter case, on two butterfly families—the Lycaenidae and Nymphalidae). We did not detect Cardinium or Hamiltonella in any host. Wolbachia were the most widespread, while Spiroplasma (ants and lepidopterans) and Arsenophonus (ants only) were present at low levels. Co-infections with different Wolbachia strains appeared especially common in ants and less so in lepidopterans. While no additional facultative heritable symbionts were found among ants using universal bacterial primers, microbes related to heritable enteric bacteria were detected in several hosts. In summary, our findings show that Wolbachia are the dominant heritable symbionts of ants and at least some lepidopterans. However, a systematic review of symbiont frequencies across host taxa revealed that this is not always the case across other arthropods. Furthermore, comparisons of symbiont frequencies revealed that the prevalence of Wolbachia and other heritable symbionts varies substantially across lower-level arthropod taxa. We discuss the correlates, potential causes, and implications of these patterns, providing hypotheses on host attributes that may shape the distributions of these influential bacteria.

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Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

A Veritable Menagerie of Heritable Bacteria from Ants, Butterflies, and Beyond: Broad Molecular Surveys and a Systematic Review

et al. 2012

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“…However, the abundance of Lactococcus in DrW was higher than those in GXSgW and GZSiW. Bacillus is a common commensal bacterium in insects and commonly found in other invertebrates and has been reported in S. invicta queens, larvae, and pupae [31,45]. Some Bacillus species are also insect pathogens and often used to control agricultural pests [46,47].…”

Section: Discussionmentioning

confidence: 99%

Identification and Diversity of Bacterial Communities Associated with the Venom Glands of the Fire Ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)

Yang

Chen

et al. 2020

Preprint

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Background Ant-microbe interactions that naturally occur in the venom microenvironment remain mostly unknown. To best of our knowledge, no research exists that shows the identity and diversity of bacterial communities in the fires ants, and what adaptive advantages that venom-associated microbes might offer to their hosts or that hosts might provide to venom-associated bacteria. This study assessed the diversity and identity of bacteria associated with the venom glands of Solenopsis invicta and compared this community with the bacterial communities of two other stinging ants, Solenopsis geminata and Diacamma rugosum. Results The major phylum associated with the venom glands of S. invicta (workers, alates, and queens) and S. geminata (workers) was Proteobacteria, while Firmicutes were abundant in the venom glands of D. rugosum (workers). Tenericutes were also more abundant in the venom glands of S. invicta queens than in those of workers and alates. The relative abundance of Spiroplasma in S. geminata was significantly higher than those in D. rugosum and S. invicta. A microbial diversity analysis of venom glands of worker ants of Solenopsis species showed that the relative abundances of Bacillus and Lactococcus were higher in Guangxi S. invicta workers than in workers collected from Guangzhou. However, the abundance of Lactococcus in workers of D. rugosum was higher than those in workers of S. geminata from Guangxi and S. invicta collected from Guangzhou. Conclusions This study provides the first insight into the microbiota in the venom glands of S. invicta, S. geminata, and D. rugosum, which might contribute to a more comprehensive view of the role of bacteria in the synthesis or degradation of active venom components in the host. We hypothesized that the differences in bacterial communities of three ant species could be due to the local adaptation of insects along with the coevolution of bacteria and hosts.

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“…8,9 As in all organisms, the development of ants requires multigene regulation, which is affected by environmental conditions such as temperature, radiation, and challenges from pesticides and parasites. [10][11][12][13][14][15][16][17][18][19][20][21][22] However, the role of GNBPs in Solenopsis invicta Buren development has never been examined. In this study, the complete cDNA of the guanine nucleotide-binding protein gene β-subunit (SiGNBP) was cloned and sequenced from S. invicta workers, and its transcriptional expression related to the developmental stages and pesticide response was examined.…”

Section: Introductionmentioning

confidence: 99%

Transcription profiling of guanine nucleotide binding proteins during developmental regulation and pesticide response in Solenopsis invicta (Hymenoptera: Formicidae)

Zhao¹,

Chen²,

Jones³

2012

OAIP

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Guanine nucleotide binding proteins (GNBP), glycoproteins anchored on the cytoplasmic cell membrane, are mediators for many cellular processes. Complete cDNA of the GNBP gene β-subunit (SiGNBP) was cloned and sequenced from Solenopsis invicta Buren (Hymenoptera: Formicidae) worker ants. To understand whether SiGNBP is developmentally regulated in S. invicta, its expression levels in different developmental stages were examined using quantitative real-time polymerase chain reaction. SiGNBP was expressed in each developmental stage, and was especially highly expressed in the late larval and early pupal stages, as well among the dealate females (queens) ∼10 days post nuptial flight. Quantitative real-time polymerase chain reaction also showed that mRNA transcription levels of SiGNBP in S. invicta workers were regulated during the duration of the study in response to heat shock, ultraviolet light, and boric acid treatment. These results suggest that the SiGNBP gene may play an important role in the development of S. invicta, and this has potential for use as a target in new insecticides for the control of fire ants.

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Identification of Bacterial Species in the Hemolymph of QueenSolenopsis invicta(Hymenoptera: Formicidae)

Cited by 11 publications

References 15 publications

A Veritable Menagerie of Heritable Bacteria from Ants, Butterflies, and Beyond: Broad Molecular Surveys and a Systematic Review

A Veritable Menagerie of Heritable Bacteria from Ants, Butterflies, and Beyond: Broad Molecular Surveys and a Systematic Review

Identification and Diversity of Bacterial Communities Associated with the Venom Glands of the Fire Ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)

Transcription profiling of guanine nucleotide binding proteins during developmental regulation and pesticide response in Solenopsis invicta (Hymenoptera: Formicidae)

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