Identification of Bacterial Micropredators Distinctively Active in a Soil Microbial Food Web

Lueders, Tillmann; Kindler, Reimo; Miltner, Anja; Friedrich, Michael W.; Kaestner, Matthias

doi:10.1128/aem.00400-06

Cited by 202 publications

(142 citation statements)

References 33 publications

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“…Furthermore, the ability of Myx. xanthus to perform gliding motility may allow it to access otherwise inaccessible prey sources (Lueders et al, 2006). Yet, the usefulness of myxobacteria to prevent the control of pests remains largely unexplored.…”

Section: Resultsmentioning

confidence: 99%

“…It is a member of the Myxobacteria, a family of organisms characterized by a diverse range of multicellular structures, as well as their cellulosic and proteolytic activities (Hyun et al, 2008). Cellulolytic myxobacteria are important to the global carbon cycle as degraders of plant biomass, while proteolytic myxobacteria are micropredators that impact carbon flow and composition of microbial communities (Lueders et al, 2006). The myxobacteria have large genomes for bacteria and produce a wide range of secondary metabolites with many undiscovered activities (Goldman et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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The predatory life cycle of Myxococcus xanthus

Keane

Berleman

2016

Microbiology

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The predatory life cycle of Myxococcus xanthus

Keane

Berleman

2016

Microbiology

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“…Fluorescent-labeled amplicons were generated as detailed elsewhere (Winderl et al, 2008). Electrophoresis of MspI-restricted fragments was carried out on an ABI 3730 DNA Analyzer (Applied Biosystems, Darmstadt, Germany), and data evaluation with the Gene Mapper 5.1 software, all as previously described (Lueders et al, 2006).…”

Section: Nucleic Acid Extraction and Ultracentrifugationmentioning

confidence: 99%

DNA-SIP identifies sulfate-reducing Clostridia as important toluene degraders in tar-oil-contaminated aquifer sediment

et al. 2010

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Global groundwater resources are constantly challenged by a multitude of contaminants such as aromatic hydrocarbons. Especially in anaerobic habitats, a large diversity of unrecognized microbial populations may be responsible for their degradation. Still, our present understanding of the respective microbiota and their ecophysiology is almost exclusively based on a small number of cultured organisms, mostly within the Proteobacteria. Here, by DNA-based stable isotope probing (SIP), we directly identified the most active sulfate-reducing toluene degraders in a diverse sedimentary microbial community originating from a tar-oil-contaminated aquifer at a former coal gasification plant. On incubation of fresh sediments with 13 C 7 -toluene, the production of both sulfide and 13 CO 2 was clearly coupled to the 13 C-labeling of DNA of microbes related to Desulfosporosinus spp. within the Peptococcaceae (Clostridia). The screening of labeled DNA fractions also suggested a novel benzylsuccinate synthase alpha-subunit (bssA) sequence type previously only detected in the environment to be tentatively affiliated with these degraders. However, carbon flow from the contaminant into degrader DNA was only B50%, pointing toward high ratios of heterotrophic CO 2 -fixation during assimilation of acetyl-CoA originating from the contaminant by these degraders. These findings demonstrate that the importance of non-proteobacterial populations in anaerobic aromatics degradation, as well as their specific ecophysiology in the subsurface may still be largely ungrasped.

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“…Terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of density-resolved DNA fractions was done with primers Ba27f-FAM and 907r; labeled amplicons were digested with MspI and size separated on a ABI 3730 DNA analyzer, all as described previously (Lueders et al, 2006).…”

Section: Fingerprinting Analysesmentioning

confidence: 99%

The use of stable isotope probing to identify key iron-reducing microorganisms involved in anaerobic benzene degradation

2007

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Here, we present a detailed functional and phylogenetic characterization of an iron-reducing enrichment culture maintained in our lab with benzene as sole carbon and energy source. We used DNA-stable isotope probing to identify microbes within the enrichment most active in the assimilation of 13 C-label. When 12 C 6 -and 13 C 6 -benzene were added as comparative substrates, marked differences in the quantitative buoyant density distribution became apparent especially for uncultured microbes within the Gram-positive Peptococcaceae, closely related to environmental clones retrieved from contaminated aquifers world wide and only distantly related to cultured representatives of the genus Thermincola. Prominent among the other constituents of the enrichment were uncultured Deltaproteobacteria, as well as members of the Actinobacteria. Although their presence within the enrichment seems to be stable they did not assimilate 13 C-label as significantly as the Clostridia within the time course of our experiment. We hypothesize that benzene degradation in our enrichment involves an unusual syntrophy, where members of the Clostridia primarily oxidize benzene. Electrons from the contaminant are both directly transferred to ferric iron by the primary oxidizers, but also partially shared with the Desulfobulbaceae as syntrophic partners. Alternatively, electrons may also be quantitatively transferred to the partners, which then reduce the ferric iron. Thus our results provide evidence for the importance of a novel clade of Gram-positive iron-reducers in anaerobic benzene degradation, and a role of syntrophic interactions in this process. These findings shed a totally new light on the factors controlling benzene degradation in anaerobic contaminated environments.

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Identification of Bacterial Micropredators Distinctively Active in a Soil Microbial Food Web

Cited by 202 publications

References 33 publications

The predatory life cycle of Myxococcus xanthus

The predatory life cycle of Myxococcus xanthus

DNA-SIP identifies sulfate-reducing Clostridia as important toluene degraders in tar-oil-contaminated aquifer sediment

The use of stable isotope probing to identify key iron-reducing microorganisms involved in anaerobic benzene degradation

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