Identification of bacterial communities in conventional wastewater treatment sludge to inform inoculation of the anammox process

Kim, Moonil; Cui, Fenghao

doi:10.1016/j.chemosphere.2022.137167

Cited by 10 publications

(4 citation statements)

References 38 publications

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“…Anammoximicrobium, Candidatus Brocadia, Candidatus Jettenia, Candidatus Kuenenia and Candidatus Scalindua, harboring more than 20 species therein, have been reported to pose anammox function [9] . Due to the environmental sensitivity of anammox bacteria, pure cultures have yet to be cultivated [10] , and it is generally assumed that anammox is accomplished by a variety of bacteria performing biological nitrogen removal [11] . Thus, the bacterial community composition determines the efficiency of biological nitrogen removal.…”

Section: Candidatusmentioning

confidence: 99%

“…A previous study indicated that, with the help of mobile genetic elements such as plasmids, integrons and transposons, ARGs can be transferred horizontally among microorganisms, causing their transmission and diffusion within the environment [14] . The microbial community diversity and structure in the biological wastewater treatment system is conducive to the selection, transfer and transmission of ARGs across a variety of bacteria [11] .…”

Section: Candidatusmentioning

confidence: 99%

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Start-up of anammox in an up-flow anaerobic sludge bed reactor: bacterial community composition and antibiotic resistance genes

Li,

Yao,

Jia

et al. 2024

Preprint

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Anaerobic ammonia oxidation (anammox) is considered a high-efficiency and low-consumption biological nitrogen removal process. In this study, synthetic wastewater was used to start an up-flow anaerobic sludge bed reactor with a starting temperature of 20–31℃, and subsequently, the activated sludge samples were used to analyze the changes in bacterial community and antibiotic resistance genes (ARGs) by metagenomic sequencing. The results showed that the reaction successfully started up after 132 days of cultivation, achieving NH4+-N and NO2−-N removal rates over 99.5%. Candidatus Kuenenia, an anammox bacterium, increased from 0.01 to 50.86%. The denitrifying bacteria Delftia, Acidovorax, Thauera and Alicycliphilus decreased from 18.70, 8.02, 4.94 and 4.59% to 7.01, 1.77, 3.06 and 3.96%, respectively. The ammonia-oxidizing bacterium Nitrosomonas decreased from 2.91 to 1.87%. After cultivation, the relative abundance of ARGs in sludge decreased from 90.23 to 64.29 ppm, with sulfonamide, macrolide-lincosamide-streptogramin, tetracycline, aminoglycoside and multidrug ARGs being the main types. Additionally, the ARG subtypes sul1, msrE and tetX decreased, while ermF, sul2 and floR increased. These results contribute to knowledge of the nitrogen removal performance, changes in bacterial community composition and ARGs in an anammox reactor, providing the guidance for the removal of ARGs by anammox.

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Section: Candidatusmentioning

confidence: 99%

Section: Candidatusmentioning

confidence: 99%

Start-up of anammox in an up-flow anaerobic sludge bed reactor: bacterial community composition and antibiotic resistance genes

Li,

Yao,

Jia

et al. 2024

Preprint

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“…As mentioned, qPCR methods for the absolute enumeration of SRB provide a fast way to overcome problems associated with microscopic and culture-dependent methods [2,2,37]. However, even successful molecular techniques require validation or verification before application in each sample, depending on their type (matrix) and complex soil/sludge type from many origins [16,36,38]. Sludge and soil samples from engineered or natural environments are characterized by their complex physical, chemical, and microbiological composition.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, sludge and soil samples host diverse microorganisms, including bacteria, fungi, archaea, viruses, and protozoa, forming intricate and interactive microbial communities. The composition and activity of these microbial populations vary across different environments, posing challenges in analyzing a specific group of microorganisms such as SRB [38,43,44].…”

Section: Introductionmentioning

confidence: 99%

Comparative Methods for Quantification of Sulfate-Reducing Bacteria in Environmental and Engineered Sludge Samples

Zambrano-Romero

Ramirez-Villacis

Barriga-Medina

et al. 2023

Biology

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This study aimed to compare microscopic counting, culture, and quantitative or real-time PCR (qPCR) to quantify sulfate-reducing bacteria in environmental and engineered sludge samples. Four sets of primers that amplified the dsrA and apsA gene encoding the two key enzymes of the sulfate-reduction pathway were initially tested. qPCR standard curves were constructed using genomic DNA from an SRB suspension and dilutions of an enriched sulfate-reducing sludge. According to specificity and reproducibility, the DSR1F/RH3-dsr-R primer set ensured a good quantification based on dsrA gene amplification; however, it exhibited inconsistencies at low and high levels of SRB concentrations in environmental and sulfate-reducing sludge samples. Ultimately, we conducted a qPCR method normalized to dsrA gene copies, using a synthetic double-stranded DNA fragment as a calibrator. This method fulfilled all validation criteria and proved to be specific, accurate, and precise. The enumeration of metabolically active SRB populations through culture methods differed from dsrA gene copies but showed a plausible positive correlation. Conversely, microscopic counting had limitations due to distinguishing densely clustered organisms, impacting precision. Hence, this study proves that a qPCR-based method optimized with dsrA gene copies as a calibrator is a sensitive molecular tool for the absolute enumeration of SRB populations in engineered and environmental sludge samples.

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Phytoremediation Processes for the Removal of Heavy Metals

El-Moustaqim,

Tallou,

Mabrouki

et al. 2024

Studies in Systems, Decision and Control

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Identification of bacterial communities in conventional wastewater treatment sludge to inform inoculation of the anammox process

Cited by 10 publications

References 38 publications

Start-up of anammox in an up-flow anaerobic sludge bed reactor: bacterial community composition and antibiotic resistance genes

Start-up of anammox in an up-flow anaerobic sludge bed reactor: bacterial community composition and antibiotic resistance genes

Comparative Methods for Quantification of Sulfate-Reducing Bacteria in Environmental and Engineered Sludge Samples

Phytoremediation Processes for the Removal of Heavy Metals

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