Identification of bacterial antigens in circulating immune complexes of infective endocarditis.

Inman, Robert D.; Redecha, Patricia; Knechtle, Stuart J.; Schned, Eric S.; Rijn, I van de; Christian, Charles L.

doi:10.1172/jci110614

Cited by 32 publications

(6 citation statements)

References 23 publications

(13 reference statements)

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“…Our findings of elevated IgG-CIC levels in both patient populations prompted us to isolate and purify CIC in the hope that preliminary characterization of individual components of those materials would provide insight into their significance. Such an approach was necessary because CIC assays are not antigen specific and provide only indirect evidence for immune complex-mediated pathogenicity (4,23). Density gradient ultracentrifugation showed that CIC from nonbacteremic patients were found mainly in or above the 19s range, which is consistent with sedimentation coefficients ofCIC found in samples from patients with other infectious diseases (5,9,47).…”

Section: Resultsmentioning

confidence: 52%

Isolation and characterization of circulating immune complexes from patients with pneumococcal pneumonia

Mellencamp¹,

Preheim²,

McDonald³

1987

Infect Immun

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Circulating immune complexes (CIC) were isolated from serum samples from patients with bacteremic and nonbacteremic pneumococcal pneumonia. Overall, 63% (26 of 41) of patients with pneumococcal pneumonia had elevated levels of immunoglobulin G (IgG)-containing CIC. IgM-containing CIC were identified in samples from only three patients. Serum samples from nonbacteremic patients contained significantly higher levels of IgG-containing CIC (96.6 111.7 ,ug/mI) than did samples from bacteremic patients (31.7 + 26.9 ,ug/ml) during week 1 in hospital (P < 0.05). Immune complex levels did not correlate with IgG concentrations in serum or anticapsular antibody levels. Immune complexes from nonbacteremic patients had sedimentation coefficients of >19s by density gradient ultracentrifugation. In contrast, CIC from bacteremic patients had smaller coefficients, of between 9s and 14s. Pneumococcal capsular antigens were identified in concentrated, dissociated CIC from both patient groups by counterimmunoelectrophoresis. recovered within 1 h and stored in 1-ml aliquots at -70°C

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Section: Resultsmentioning

confidence: 52%

Isolation and characterization of circulating immune complexes from patients with pneumococcal pneumonia

Mellencamp¹,

Preheim²,

McDonald³

1987

Infect Immun

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show abstract

“…Identification and characterization of host and bacterial components in circulating IC appear to be especially important for understanding their pathogenetic significance in disease (26,27). Although considerable attention has been directed at establishing the presence of IC in patients with secondary syphilis (14,41,46,47) and rabbits with disseminated T. pallidum infection (6,8), attempts to characterize the individual components of those IC have not been described.…”

Section: Discussionmentioning

confidence: 99%

Circulating immune complexes in experimental syphilis: identification of treponemal antigens and specific antibodies to treponemal antigens in isolated complexes

Baughn¹,

Adams²,

Musher³

1983

Infect Immun

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As a prelude to characterization of the host and treponemal antigens present in purified immune complexes from the sera of rabbits with disseminated syphilis, autoradiographic and immunoenzymatic analyses of solubilized extracts of Treponema pallidum, Treponema phagedenis biotype Reiter, and Treponema refringens were performed on electroblots of polypeptides first separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Electroblots of purified immune complexes were developed with the same panel of antisera so that protein profiles could be compared. Eight treponemal antigens were consistently present in isolated complexes; four of these cross-reacted with antisera prepared against avirulent treponemes. The average molecular weights of these antigens were 87,000, 76,000, 66,000, and 45,000. Antibodies dissociated from isolated immune complexes, when used for the development of T. pallidum electroblots, reacted with four antigens of comparable molecular weight. Antibodies to those polypeptides were also present in the sera of animals immunized with immune complexes. The demonstration of treponemal antigens in purified immune complexes convincingly argues that their occurrence in experimental syphilis is not merely due to tissue destruction and responses to endogenous host antigens.

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“…Further, the authors may have been unaware of several studies where pathogen-specific antibody has been found in IC in infectious diseases prior to LD (5)(6)(7)(8)11).…”

Section: Report Refuting Value Of Immune Complexes To Diagnose Lyme Dmentioning

confidence: 99%

Report Refuting Value of Immune Complexes To Diagnose Lyme Disease Is Invalid

Brunner¹

2006

Clin Vaccine Immunol

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Identification of bacterial antigens in circulating immune complexes of infective endocarditis.

Cited by 32 publications

References 23 publications

Isolation and characterization of circulating immune complexes from patients with pneumococcal pneumonia

Isolation and characterization of circulating immune complexes from patients with pneumococcal pneumonia

Circulating immune complexes in experimental syphilis: identification of treponemal antigens and specific antibodies to treponemal antigens in isolated complexes

Report Refuting Value of Immune Complexes To Diagnose Lyme Disease Is Invalid

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