Identification of bacteria using mass spectrometry

Anhalt, John P.; Fenselau, Catherine

doi:10.1021/ac60352a007

Cited by 436 publications

(246 citation statements)

References 38 publications

(9 reference statements)

Supporting

Mentioning

235

Contrasting

Unclassified

Order By: Relevance

“…Bacterial identification based on peptidic spectra obtained by MALDI-TOF mass spectrometry was proposed >30 years ago (Anhalt and Fenselau, 1975;Claydon et al, 1996;Krishnamurthy and Ross, 1996). It has only recently been used as a rapid, inexpensive, and accurate method for identifying bacteria.…”

Section: Review Protein Cellmentioning

confidence: 99%

The role of gut microbiota in the gut-brain axis: current challenges and perspectives

2013

View full text Add to dashboard Cite

Brain and the gastrointestinal (GI) tract are intimately connected to form a bidirectional neurohumoral communication system. The communication between gut and brain, knows as the gut-brain axis, is so well established that the functional status of gut is always related to the condition of brain. The researches on the gut-brain axis were traditionally focused on the psychological status affecting the function of the GI tract. However, recent evidences showed that gut microbiota communicates with the brain via the gut-brain axis to modulate brain development and behavioral phenotypes. These recent fi ndings on the new role of gut microbiota in the gut-brain axis implicate that gut microbiota could associate with brain functions as well as neurological diseases via the gut-brain axis. To elucidate the role of gut microbiota in the gut-brain axis, precise identification of the composition of microbes constituting gut microbiota is an essential step. However, identifi cation of microbes constituting gut microbiota has been the main technological challenge currently due to massive amount of intestinal microbes and the diffi culties in culture of gut microbes. Current methods for identifi cation of microbes constituting gut microbiota are dependent on omics analysis methods by using advanced high tech equipment. Here, we review the association of gut microbiota with the gut-brain axis, including the pros and cons of the current high throughput methods for identifi cation of microbes constituting gut microbiota to elucidate the role of gut microbiota in the gut-brain axis.

show abstract

Section: Review Protein Cellmentioning

confidence: 99%

The role of gut microbiota in the gut-brain axis: current challenges and perspectives

2013

View full text Add to dashboard Cite

show abstract

“…Principle (Figure 1) The first description concerning the use of MS in bacteriology was in 1975 [3] . The technology was developed to study the biomarker profiles of some bacterial species.…”

Section: The Maldi-tof Ms Technologymentioning

confidence: 99%

Mass spectrometry: a revolution in clinical microbiology?

Lavigne

Espinal

Dunyach-Rémy

et al. 2012

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

Recently, different bacteriological laboratory interventions that decrease reporting time have been developed. These promising new broad-based techniques have merit, based on their ability to identify rapidly many bacteria, organisms difficult to grow or newly emerging strains, as well as their capacity to track disease transmission. The benefit of rapid reporting of identification and/or resistance of bacteria can greatly impact patient outcomes, with an improvement in the use of antibiotics, in the reduction of the emergence of multidrug resistant bacteria and in mortality rates. Different techniques revolve around mass spectrometry (MS) technology: matrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI-TOF MS), PCR combined with electrospray ionization-mass spectrometry (PCR/ESI-MS), iPLEX MassArray system and other new evolutions combining different techniques. This report emphasizes the (r)evolution of these technologies in clinical microbiology.

show abstract

“…La spectrométrie de masse (MS), technique utilisée depuis la fin du XIX e siècle, a été appliquée dès les années 1970 à l'identification de micro-organismes [2]. Le développement des techniques de spectrométrie de masse à ionisation douce comme le MALDI-TOF (matrix assisted laser desorption ionisation time-of-flight) ou l'ESI (electrospray ionisation) a permis l'analyse d'un large panel de biomarqueurs et l'essor de cette technologie [3,33].…”

Section: Application De La Spectrométrie De Masse à L'identification unclassified

Utilisation en routine du MALDI-TOF-MS pour l’identification des pathogènes en microbiologie médicale

Carbonnelle

Nassif

2011

Med Sci (Paris)

View full text Add to dashboard Cite

Identification of bacteria using mass spectrometry

Cited by 436 publications

References 38 publications

The role of gut microbiota in the gut-brain axis: current challenges and perspectives

The role of gut microbiota in the gut-brain axis: current challenges and perspectives

Mass spectrometry: a revolution in clinical microbiology?

Utilisation en routine du MALDI-TOF-MS pour l’identification des pathogènes en microbiologie médicale

Contact Info

Product

Resources

About