Identification of Bacillus anthracis from Culture Using Gas Chromatographic Analysis of Fatty Acid Methyl Esters

Sasser, Myron; Kunitsky, C; Jackoway, Gary; Jw, Ezzell; Jd, Teska; Harper, B; Parker, Steve H.; Barden, David; Blair, Heather; Breezee, Jennifer L.; Carpenter, J. A.; Wv, Cheek; DeMartino, M; Evans, Bonnie; Francesconi, Sandro; Franko, Evgenia; Gardner, W. U.; Glazier, M; Greth, Kimberly M.; Hart, Tony; M, Hodel; Holmes-Talbot, K; Kl, Hopkins; Iqbal, Arfa; Johnson, Desiree R.; Krader, Paul; Madonna, Angelo J.; McDowell, Margaret; Ml, McKee; M, Park; Pentella, M; Radosevic, Jon; Robison, Richard A.; Rotzoll, B; Scott, Karen S.; Smith, M. D.; Syed, Noman; Tang, Jing; Trinh, Ha Thu; Li, Williams; Wolcott, Michael P.

doi:10.1093/jaoac/88.1.178

Cited by 56 publications

(26 citation statements)

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“…For each isolate, after 2 days of incubation, two aliquots with roughly 25-70 mg of bacterial biomass per tube were prepared. FAME preparation and GC/ MS analyses were performed as already reported Sasser, 2006 The susceptibility of all three isolates was tested for 11 antibiotics: amoxicillin (0.16-256 μg/ml), benzylpenicillin (0.002-32 μg/ ml), ceftriaxone (0.002-32 μg/ml), ertapenem (0.002-32 μg/ml), imipenem (0.002-32 μg/ml), amikacin (0.16-256 μg/ml), erythromycin (0.16-256 μg/ml), metronidazole (0.16-256 μg/ml), ofloxacin (0.002-32 μg/ml), rifampicin (0.002-32 μg/ml), and vancomycin (0.16-256 μg/ml). Minimal inhibitory concentrations (MICs) were estimated using E-test strips (bioMérieux) and according to EUCAST recommendations (Citron, Ostovari, Karlsson, & Goldstein, 1991;Matuschek, Brown, & Kahlmeter, 2014).…”

Section: Phenotypic Characteristicsmentioning

confidence: 99%

Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.

Diop

Michelle

et al. 2018

MicrobiologyOpen

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Three previously unidentified Gram-positive anaerobic coccoid bacteria, strains KhD-2 , KHD4 , and Kh-D5 , isolated from a vaginal swab, were characterized using the taxonogenomics concept. The phylogenic analysis, phenotypic characteristics, and genotypic data presented in this report attest that these three bacteria are distinct from previously known bacterial species with standing in nomenclature and represent three new Peptoniphilus species. Strain KhD-2 is most closely related to Peptoniphilus sp. DNF00840 and Peptoniphilus harei (99.7% and 98.2% identity, respectively); strain KHD4 to Peptoniphilus lacrimalis (96%) and strain Kh-D5 to Peptoniphilus coxii (97.2%). Strains KhD-2 , KHD4 , and Kh-D5 DNA G+C contents are, respectively, 34.23%, 31.87%, and 49.38%; their major fatty acid was C (41.6%, 32.0%, and 36.4%, respectively). We propose that strains KhD-2 (=CSUR P0125 = DSM 101742), KHD4 (=CSUR P0110 = CECT 9308), and Kh-D5 (=CSUR P2271 = DSM 101839) be the type strains of the new species for which the names Peptoniphilus vaginalis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilu pacaensis sp. nov., are proposed, respectively.

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Section: Phenotypic Characteristicsmentioning

confidence: 99%

Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.

Diop

Michelle

et al. 2018

MicrobiologyOpen

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“…Two bacterial biomass sample tubes of about 4 mg each obtained from cultures plates were prepared after 72 hr of culture of the bacteria on 5% sheep blood-enriched Columbia agar (bioMérieux) in anaerobic conditions. Then, fatty acid methyl esters were prepared according to the description of Sasser (2006). GC/MS analyses were carried out as previously stated .…”

Section: Fatty Acid Methyl Ester Analysismentioning

confidence: 99%

Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2^T, the second Intestinimonas species cultured from the human gut

et al. 2018

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Intestinimonas massiliensis sp. nov strain GD2 is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono-genomics, it is strictly anaerobic, nonspore-forming, rod-shaped, with catalase- and oxidase-negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched with sheep blood (5%) and rumen fluid (5%), incubated at 37°C. Its phenotypic and genotypic descriptions are presented in this paper with a full annotation of its genome sequence. This genome consists of 3,104,261 bp in length and contains 3,074 predicted genes, including 3,012 protein-coding genes and 62 RNA-coding genes. Strain GD2 significantly produces butyrate and is frequently found among available 16S rRNA gene amplicon datasets, which leads consideration of Intestinimonas massiliensis as an important human gut commensal.

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“…The cellular fatty acids were extracted, and fatty acid methyl esters (FAMEs) were derived according to the procedure described previously (Sasser et al, 2005). In the present study, FAMEs were analyzed by GC/mass spectrometer (MS) using Agilent 5975C Series Gas Chromatograph/Mass Selective Detector Systems complemented by 7890A GC.…”

Section: Analysis Of Cellular Fatty Acidsmentioning

confidence: 99%

Visualized analysis of cellular fatty acid profiles ofVibrio parahaemolyticusstrains under cold stress

Jia

Chen

Jiang

et al. 2014

FEMS Microbiol Lett

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Vibrio parahaemolyticus is a common foodborne bacterial pathogen, which survives in cold environments and is sometimes difficult to culture. Fatty acid analysis under cold stress was conducted for several V. parahaemolyticus strains using gas chromatography/mass spectrometry, and the results were compared with those of the controls. All the fatty acid profiles obtained were visualized by multidimensional scaling (MDS) and self-organized map (SOM). It was observed that the fatty acid profiles of V. parahaemolyticus substantially changed under cold stress. The percentage of methyl palmitate remarkably decreased and that of methyl palmitoleate (except for two strains) and methyl oleate increased. These findings demonstrate the role of fatty acids in cold stress. The changes in the fatty acid profiles illustrated by MDS and SOM could differentiate strains under cold stress from the controls and can potentially lead to a method of detecting injured cold-stressed V. parahaemolyticus.

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Identification of Bacillus anthracis from Culture Using Gas Chromatographic Analysis of Fatty Acid Methyl Esters

Cited by 56 publications

References 0 publications

Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.

Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.

Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2^T, the second Intestinimonas species cultured from the human gut

Visualized analysis of cellular fatty acid profiles ofVibrio parahaemolyticusstrains under cold stress

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Identification of Bacillus anthracis from Culture Using Gas Chromatographic Analysis of Fatty Acid Methyl Esters

Cited by 56 publications

References 0 publications

Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.

Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.

Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2T, the second Intestinimonas species cultured from the human gut

Visualized analysis of cellular fatty acid profiles ofVibrio parahaemolyticusstrains under cold stress

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Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2^T, the second Intestinimonas species cultured from the human gut