Identification of Arabidopsis VTC3 as a putative and unique dual function protein kinase::protein phosphatase involved in the regulation of the ascorbic acid pool in plants

Conklin, Patricia L.; DePaolo, Dennis; Wintle, Brittie; Schatz, Carmit; Buckenmeyer, G K

doi:10.1093/jxb/ert140

Cited by 47 publications

(51 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biosynthesis of AsA in plants is postulated to be regulated by several enzymes in the pathway, namely, GalLDH (Yabuta et al, 2007), GDP-D-mannose pyrophosphorylase (VTC1; Conklin et al, 1997, 1999; Veljovic-Jovanovic et al, 2001; Badejo et al, 2007, 2008; Wang et al, 2013), GDP-D-mannose 3′, 5′-epimerase (GME) in association with GDP-L-galactose phosphorylase (VTC2/VTC5; Dowdle et al, 2007; Wolucka and Van Montagu, 2007; Linster and Clarke, 2008; Bulley et al, 2009), the predicted F-box protein AMR1 (Zhang et al, 2009) and the protein kinase::protein phosphatase VTC3 (Conklin et al, 2013). These works are mainly based on the analysis of A. thaliana mutants defective in the production of AsA, or on correlations between the levels of AsA and gene expression of these enzymes in other plant species.…”

Section: Discussionmentioning

confidence: 99%

“…The steady state level of transcripts encoding several of the Smirnoff-Wheeler biosynthetic enzymes has been shown to correlate with exposure to light and AsA content (Gatzek et al, 2002; Tamaoki et al, 2003; Bartoli et al, 2006, 2009; Dowdle et al, 2007; Maruta et al, 2008; Yabuta et al, 2008; Fukunaga et al, 2010). Besides this light-dependent regulation, two genes have been identified as regulators of the pathway in A. thaliana : AMR1, a predicted F-box protein (Zhang et al, 2009) and VTC3, a protein kinase::protein phosphatase (Conklin et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Drought Stress Causes a Reduction in the Biosynthesis of Ascorbic Acid in Soybean Plants

et al. 2017

View full text Add to dashboard Cite

Drought provokes a number of physiological changes in plants including oxidative damage. Ascorbic acid (AsA), also known as vitamin C, is one of the most abundant water-soluble antioxidant compound present in plant tissues. However, little is known on the regulation of AsA biosynthesis under drought stress conditions. In the current work we analyze the effects of water deficit on the biosynthesis of AsA by measuring its content, in vivo biosynthesis and the expression level of genes in the Smirnoff-Wheeler pathway in one of the major legume crop, soybean (Glycine max L. Merr). Since the pathway has not been described in legumes, we first searched for the putative orthologous genes in the soybean genome. We observed a significant genetic redundancy, with multiple genes encoding each step in the pathway. Based on RNA-seq analysis, expression of the complete pathway was detected not only in leaves but also in root tissue. Putative paralogous genes presented differential expression patterns in response to drought, suggesting the existence of functional specialization mechanisms. We found a correlation between the levels of AsA and GalLDH biosynthetic rates in leaves of drought-stressed soybean plants. However, the levels of GalLDH transcripts did not show significant differences under water deficit conditions. Among the other known regulators of the pathway, only the expression of VTC1 genes correlated with the observed decline in AsA in leaves.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Drought Stress Causes a Reduction in the Biosynthesis of Ascorbic Acid in Soybean Plants

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Thus, high content of galactose accumulated in atdfb-3 seeds might promote germination while mannose inhibits storage mobilization. Besides, mannose and galactose are intermediates of ascorbic acid biosynthesis [38]. Therefore, it needs further investigation that whether the accumuation of mannose and galactose is due to deficient ascorbic acid biosynthesis in atdfb-3 .…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness

Meng

Ling

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

Interactions among metabolic pathways are important in plant biology. At present, not much is known about how folate metabolism affects other metabolic pathways in plants. Here we report a T-DNA insertion mutant (atdfb-3) of the plastidial folylpolyglutamate synthetase gene (AtDFB) was defective in seed reserves and skotomorphogenesis. Lower carbon (C) and higher nitrogen (N) content in the mutant seeds than that of the wild type were indicative of an altered C and N partitioning capacity. Higher levels of organic acids and sugars were detected in the mutant seeds compared with the wild type. Further analysis revealed that atdfb-3 seeds contained less total amino acids and individual Asn and Glu as well as NO3 −. These results indicate significant changes in seed storage in the mutant. Defects in hypocotyl elongation were observed in atdfb-3 in darkness under sufficient NO3 − conditions, and further enhanced under NO3 − limited conditions. The strong expression of AtDFB in cotyledons and hypocotyl during early developmental stage was consistent with the mutant sensitivity to limited NO3 − during a narrow developmental window. Exogenous 5-formyl-tetrahydrofolate completely restored the hypocotyl length in atdfb-3 seedlings with NO3 − as the sole N source. Further study demonstrated that folate profiling and N metabolism were perturbed in atdfb-3 etiolated seedlings. The activity of enzymes involved in N reduction and assimilation was altered in atdfb-3. Taken together, these results indicate that AtDFB is required for seed reserves, hypocotyl elongation and N metabolism in darkness, providing novel insights into potential associations of folate metabolism with seed reserve accumulation, N metabolism and hypocotyl development in Arabidopsis.

show abstract

“…VTC3 was recently found to encode an unusual and novel polypeptide with an N-terminal protein kinase domain tethered covalently to a C-terminal protein phosphatase type 2C domain. 47) The VTC3 protein was localized in chloroplasts, and its knockout inhibited the accumulation of AsA in response to light, which suggested a role for this protein in the light-and PET-dependent regulation of the D-Man/L-Gal pathway. In addition to light, phytohormones, such as jasmonic acid and ethylene, are likely to regulate the biosynthesis of AsA; however, their contribution to the light regulation of AsA biosynthesis remains unclear.…”

Section: Distribution and Biosynthesis Of Asamentioning

confidence: 93%

Cellular redox regulation, signaling, and stress response in plants

Shigeoka

Maruta

2014

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Cellular and organellar redox states, which are characterized by the balance between oxidant and antioxidant pool sizes, play signaling roles in the regulation of gene expression and protein function in a wide variety of plant physiological processes including stress acclimation. Reactive oxygen species (ROS) and ascorbic acid (AsA) are the most abundant oxidants and antioxidants, respectively, in plant cells; therefore, the metabolism of these redox compounds must be strictly and spatiotemporally controlled. In this review, we provided an overview of our previous studies as well as recent advances in (1) the molecular mechanisms and regulation of AsA biosynthesis, (2) the molecular and genetic properties of ascorbate peroxidases, and (3) stress acclimation via ROS-derived oxidative/redox signaling pathways, and discussed future perspectives in this field.

show abstract

Identification of Arabidopsis VTC3 as a putative and unique dual function protein kinase::protein phosphatase involved in the regulation of the ascorbic acid pool in plants

Cited by 47 publications

References 62 publications

Drought Stress Causes a Reduction in the Biosynthesis of Ascorbic Acid in Soybean Plants

Drought Stress Causes a Reduction in the Biosynthesis of Ascorbic Acid in Soybean Plants

Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness

Cellular redox regulation, signaling, and stress response in plants

Contact Info

Product

Resources

About