Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus

Zhang, Yibing; Zhang, Qi-Ya; Xu, Dong; Hu, Chengyu; Gui, Jian‐Fang

doi:10.1360/03tb9124

Cited by 56 publications

(87 citation statements)

References 22 publications

(25 reference statements)

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“…A complete blockade occurred when 20 ng/ml of rIFN was used in that no virus titer was detected. In line with the rIFN antiviral effect, a number of important genes, which are predicated to participate in fish IFN response (Zhang et al, 2007;Zhang et al, 2003b), were significantly induced upon rIFN treatment, even with 0.1 ng/ml of rIFN (Fig. 4B).…”

Section: Crucian Carp Ifn-induced Protection Is Concomitant With Uprementioning

confidence: 54%

“…These gene counterparts also exist in fish genome (Sarkar et al, 2008;Zhang et al, 2007;Zou et al, 2009). Actually, some important genes, including RIG-I, Mda5, MITA, TBK1, IRF3 and IRF7, which are known to play essential roles in virus-activated mammalian IFN response (Tamura et al, 2008), have been identified in crucian carp (Jiang et al, 2009;Zhang et al, 2003a;Zhang et al, 2007;Zhang et al, 2003b). Our preliminary data showed that there exists a RIG-I/Mda5-MITA-TBK1-IRF3/IRF7 pathway that is responsible for IFN induction (unpublished data).…”

Section: Discussionmentioning

confidence: 68%

“…Either transfection of the IFN construct or treatment with rIFN significantly activated a strong host antiviral state and the expression of a set of genes, which are the orthologues of mammalian ISG (Zhang et al, 2007;Zhang et al, 2003b). However, under both conditions, both the host antiviral state and the expression of these genes were significantly blocked by polyclonal anti-IFN antibody.…”

Section: Discussionmentioning

confidence: 98%

“…This study aimed to investigate the function of a crucian carp (Carassius auratus L.) IFN by asking how it exerts its antiviral effect. We have previously identified by suppressive subtractive hybridization a set of genes, which are the counterparts of key genes involved in mammalian IFN antiviral response (Zhang et al, 2007;Zhang et al, 2003b). In the current study, we show that crucian carp IFN belongs to group I of fish virus-induced IFNs, and similar to mammalian IFNs.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fish virus-induced interferon exerts antiviral function through Stat1 pathway

Zhang

Liu

et al. 2010

Molecular Immunology

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Section: Crucian Carp Ifn-induced Protection Is Concomitant With Uprementioning

confidence: 54%

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fish virus-induced interferon exerts antiviral function through Stat1 pathway

Zhang

Liu

et al. 2010

Molecular Immunology

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“…An SSH library between embryos at blastula and tail bud stage was constructed as described previously (Shi et al, 2002;Xie et al, 2003;Zhang et al, 2003). Briefly, double strand cDNAs synthesized from 2 μg polyA + RNA of blastula and tail bud stage embryos were subjected to blunt-end digestion by RsaI.…”

Section: Subtractive Suppression Hybridizationmentioning

confidence: 99%

Identification of a novel C2 domain factor in ovaries of orange-spotted grouper (Epinephelus coioides)

Ji¹,

Zhou²,

Wang³

et al. 2006

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Differential expression of two Carassius auratus Mx genes in cultured CAB cells induced by grass carp hemorrhage virus and interferon

Zhang

Gui

2004

Immunogenetics

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UV-inactivated GCHV (grass carp hemorrhage virus) is able to induce an antiviral state in cultured CAB cells (crucian carp Carassius auratus blastulae embryonic cells) via the production of interferon (IFN). In the current work, the full-length cDNAs of two Mx genes, termed CaMx1 and CaMx2, have been cloned and sequenced from UV-inactivated GCHV-infected and still IFN-producing CAB cells by suppression subtractive hybridization. Their putative proteins show the characteristically structural features of mammalian IFN-induced Mx proteins, including GTP-binding motif, dynamin family signature and leucine zipper motif. CaMx1 exhibits 85% sequence identity to zebrafish MxA and 72-74% to three Atlantic salmon Mx proteins. CaMx2 is most similar to zebrafish MxE, with 80% identity, and then rainbow trout Mx3, with 52%. Constitutive expression was detected by RT-PCR for CaMx1, but not for CaMx2, in normal CAB cells, but their up-regulations could be induced after treatment with active GCHV, UV-inactivated GCHV and CAB IFN. Distinct kinetics of expression was observed for either CaMx1 or CaMx2 corresponding to the three stimuli, and even between CaMx1 and CaMx2, corresponding to the same stimulus. Upon virus infection, the transcriptional induction was strongly blocked for CaMx2 by cycloheximide (CHX), whereas almost nothing was observed for CaMx1. By contrast, following treatment with CAB IFN, CHX did not inhibit either gene transcription. Collectively, these results suggest that there are very distinct mechanisms for modulating the expression of both CaMx1 and CaMx2 in normal and GCHV-infected CAB cells.

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Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus

Cited by 56 publications

References 22 publications

Fish virus-induced interferon exerts antiviral function through Stat1 pathway

Fish virus-induced interferon exerts antiviral function through Stat1 pathway

Identification of a novel C2 domain factor in ovaries of orange-spotted grouper (Epinephelus coioides)

Differential expression of two Carassius auratus Mx genes in cultured CAB cells induced by grass carp hemorrhage virus and interferon

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