Abstract:The main objective of the current study was to investigate the components and antioxidant activity of essential oil extracted from Purple Eleutherococcus leaves (identified as Eleutherococcus simonii) (PEEO) by hydrodistillation (HD) and solid-phase microextraction (SPME). Fourteen chemical components were identified in PEEO using gas chromatography-mass spectrometry (GC-MS), accounting for 41.21% of the total essential oil extracted by SPME, including isopropyl toluene, 4-methylene-1-(1-methylethyl) cyclohexe… Show more
“…The antioxidant activity of MFO was measured in terms of radical scavenging ability, using a DPPH plant assay. All measurements were performed as follows: an amount of 1.0 mL of the five different concentrations of liquid extract was added to 2.0 mL of the DPPH• ethanol solution with a mass concentration of 0.1 mmol L -1 , and the absorbance readings were taken after 30 min against the blank at 517 nm (Zhang et al, 2022b;Nie et al, 2022). The antioxidant capacity was calculated using the following Equation 1where, (Ac: the absorbance of DPPH• solution and solvent, Ai: the absorbance of the sample solution and DPPH• solution, Aj: the absorbance of solvent solution and the sample): 2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) free radical scavenging assay ABTS• + solution (7 mmol/L) and potassium persulfate solution (2.45 mmol/L) should be mixed in equal amounts.…”
Coronary heart disease (CHD) is one of the main diseases causing morbidity and mortality globally, with oxidative damage and lipid peroxidation associated with oxidative stress and atherosclerosis. Cinnamomum migao is a Miao ethnomedicine that has been shown to provide relief from CHD symptoms. A gas chromatograph-mass spectrometer (GC-MS) was used to determine the chemical components of ten batches of migao fatty oil (MFO), yielding a total of 35 chemical constituents. The antioxidant activity of MFO in vitro was assessed using the DPPH• and ABTS• + assay methods, and both indicated good antioxidant activity. The network pharmacology predicted the active constituents of MFO and their therapeutic influence on the mechanism of oxidative damage-induced coronary heart disease (OD-CHD). Six compounds were discovered in MFO, including tau-cadinol acetate and dodecanoic acid, which may play a role in OD-CHD by acting on lipids and atherosclerosis, the PI3K-Akt signalling pathway, and other pathways. In conclusion, our study lays the foundation for investigating the use of MFO in the treatment of OD-CHD.
“…The antioxidant activity of MFO was measured in terms of radical scavenging ability, using a DPPH plant assay. All measurements were performed as follows: an amount of 1.0 mL of the five different concentrations of liquid extract was added to 2.0 mL of the DPPH• ethanol solution with a mass concentration of 0.1 mmol L -1 , and the absorbance readings were taken after 30 min against the blank at 517 nm (Zhang et al, 2022b;Nie et al, 2022). The antioxidant capacity was calculated using the following Equation 1where, (Ac: the absorbance of DPPH• solution and solvent, Ai: the absorbance of the sample solution and DPPH• solution, Aj: the absorbance of solvent solution and the sample): 2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) free radical scavenging assay ABTS• + solution (7 mmol/L) and potassium persulfate solution (2.45 mmol/L) should be mixed in equal amounts.…”
Coronary heart disease (CHD) is one of the main diseases causing morbidity and mortality globally, with oxidative damage and lipid peroxidation associated with oxidative stress and atherosclerosis. Cinnamomum migao is a Miao ethnomedicine that has been shown to provide relief from CHD symptoms. A gas chromatograph-mass spectrometer (GC-MS) was used to determine the chemical components of ten batches of migao fatty oil (MFO), yielding a total of 35 chemical constituents. The antioxidant activity of MFO in vitro was assessed using the DPPH• and ABTS• + assay methods, and both indicated good antioxidant activity. The network pharmacology predicted the active constituents of MFO and their therapeutic influence on the mechanism of oxidative damage-induced coronary heart disease (OD-CHD). Six compounds were discovered in MFO, including tau-cadinol acetate and dodecanoic acid, which may play a role in OD-CHD by acting on lipids and atherosclerosis, the PI3K-Akt signalling pathway, and other pathways. In conclusion, our study lays the foundation for investigating the use of MFO in the treatment of OD-CHD.
“…Their roots and stem barks have traditionally been used as a tonic, as well as in the treatment of rheumatism and diabetes, chronic, bronchitis and so on (Li et al, 2016;Zou, et al, 2017;Chinese Pharmacopoeia Commission, 2015). Moreover, their leaves and roots are popularly used as a health drink and drug in China and Korea (Yook et al, 1999;Hu et al, 2012;Hu et al, 2018a;Nie et al, 2021;Chen et al, 2021). Acanthopanax leucorrhizus (Oliv.)…”
Acanthopanax leucorrhizus is an endemic medicinal plant growing abundantly in the northwest region of China. The roots and stem barks have been widely used to treat rheumatism, numbness, contracture, quadriplegia, hemiplegia, traumatic injury, edema, and itchy skin, although the anti-inflammatory effects of the extracts of A. leucorrhizus have not been assessed until now. To investigate the anti-inflammatory effects on macrophages of various extracts from A. leucorrhizus stems, alterations in the inducible nitric oxide synthase (iNOS)-mediated cyclooxygenase-2 (COX-2) expression and inflammatory cytokine production were measured in lipopolysaccharide (LPS)-activated RAW264.7 cells. Briefly, the dichloromethane extract prepared from stems of A. leucorrhizus (ALSDC) effectively inhibited NO production in LPS-stimulated cells and significantly reduced the production of pro-inflammatory cytokines IL-6 and TNF-α at a dose of 40 µg/mL. We also confirmed a dose-dependent and significant inhibition of iNOS and COX-2 protein expression. In conclusion, ALSDC exerted strong inhibitory effect on the expression of iNOS and COX-2 protein in LPS-induced RAW 264.7 macrophages and these results provide strong evidence to suggest that ALSDC may be considered as an important candidate for the treatment of inflammatory-related diseases in the future.
Eleutherococcus divaricatus (Siebold and Zucc.) S. Y. Hu. has been used in Traditional Chinese Medicine (TCM) due to its anticancer, immunostimulant, and anti-inflammatory activities. However, its mechanism of action and chemical composition are still insufficiently understood and require more advanced research, especially for cases in which anti-inflammatory properties are beneficial. The aim of this study was to evaluate the impact of E. divaricatus root extracts and fractions on proinflammatory serum hyaluronidase and tyrosinase in children diagnosed with acute lymphoblastic leukemia. Antioxidant and anti-melanoma activities were also examined and correlated with metabolomic data. For the first time, we discovered that the ethyl acetate fraction significantly inhibits hyaluronidase activity, with mean group values of 55.82% and 63.8% for aescin used as a control. However, interestingly, the fraction showed no activity against human tyrosinase, and in A375 melanoma cells treated with a doxorubicin fraction, doxorubicin activity decreased. This fraction exhibited the most potent antioxidant activity, which can be attributed to high contents of polyphenols, especially caffeic acid (24 mg/g). The findings suggest an important role of the ethyl acetate fraction in hyaluronidase inhibition, which may additionally indicate its anti-inflammatory property. The results suggest that this fraction can be used in inflammatory-related diseases, although with precautions in cases of patients undergoing chemotherapy.
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