Identification of Antioxidant and Antimicrobial Compounds of <i>Lippia</i> Species by Bioautography

Fabri, Rodrigo Luiz; Nogueira, Mauro S.; Moreira, Jussara dos Reis; Bouzada, Maria Lúcia M.; Scio, Elita

doi:10.1089/jmf.2010.0141

Cited by 27 publications

(16 citation statements)

References 21 publications

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“…The technique used to determine these antifungal activities was bio-autography and indicated that the antioxidant activity of the extracts was due to coumarins and flavonoids. Terpenoids and the same flavonoids were associated to the observed antimicrobial properties (Fabri, Nogueira, Moreira, Bouzada, & Scio, 2011). Our results confirmed the antimicrobial potential of Lippia spp.…”

Section: Antifungal Assaysupporting

confidence: 84%

Chemical and antifungal investigations of six Lippia species (Verbenaceae) from Brazil

et al. 2012

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The Lippia genus is used in ethnobotany as food, beverages, seasoning and antiseptic remedies, among others. The chemical compositions of fifteen extracts of six Lippia species were investigated comparatively by HPLC-PDA. To avoid data replication of previous works on this genus, Lippia lupulina Cham. root ethanol extract was selected for isolation procedures based on Principal Component Analyses (PCA) of such data. Seven compounds previously unreported in this genus were isolated from this extract (a triterpene, two furanonaphtoquinones, a furanochromone, an isoflavone, a stilbene and an iridoid). The activities of extracts, fractions and pure compounds towards Candida albicans, Candida krusei, Candida parapsilosis and Cryptococcus neoformans were investigated. Two fractions from the extract of Lippia salviaefolia leaves showed marked inhibition of fungal growth, in addition to verbascoside and asebogenin, which showed MICs lower than 15.6μg/ml and may be promising leads for the development of new antifungal agents, especially against C. neoformans.

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Section: Antifungal Assaysupporting

confidence: 84%

Chemical and antifungal investigations of six Lippia species (Verbenaceae) from Brazil

et al. 2012

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“…Timol and DMSO (the vehicle) were tested at the same concentrations used for EOLC. The tubes were kept at room temperature and mortality was evaluated after 24 h (Fabri et al, 2011). Statistical analysis to adjust death percentage was performed using Wilcoxon and Lichfield method through Probit analysis (Finney, 1971), permitting the determination of LC50 (50% lethal concentration of nauplii).…”

Section: Hatching Of Brine Shrimp Larvaementioning

confidence: 99%

Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Machado

Valente²,

Lesche³

et al. 2012

Rev. bras. farmacogn.

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Abstract:Leishmaniasis is an infection of viscera or tegument caused by protozoa Leishmania sp. The extensive period required for the treatment, which involves the use of toxic medicines, leads patients to drop treatment increasing the development of resistant forms of Leishmania sp. Lantana camara L., Verbenaceae, is a tropical plant native from America. Folk uses have been described for treatment of tumors, tetanus, rheumatism and malaria. This study evaluates the leishmanicidal activity of the essential oil of leaves from L. camara on promastigote forms of Leishmania chagasi and L. amazonensis and its toxic effects on Artemia salina (brine shrimp test), macrophage cultures and BALB/c mice. The chemical composition was evaluated using the gas chromatography coupled with mass spectrometer (GC-MS). Thirty substances, mostly mono and sesquiterpenes were identifi ed. The most representative constituents were: germacrene D (24.90%), farnesene derivatives (22%) and (E)-cariophylene (14.31%). Bioassays revealed a signifi cant leishmanicidal activity of essential oil against L. amazonensis (IC50 0.25 μg/ mL) and a potential toxic effect on Brine shrimp (LC50 10 μg/mL) and macrophage assays (CC50 4 μg/mL), while there was no toxic manifestation on mice. The data show the relevant potential of L. camara as a source of medicine for leishmaniasis treatment.

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“…Studies have shown the essential oil of L. sidoides have antiparasitic action against ticks (Gomes et al, 2012(Gomes et al, , 2014, forms of Leishmania (Oliveira et al, 2009;Farias-Júnior et al, 2012), helminths from caprine and ovine animals (Camurç a-Vasconcelos et al, 2007) and monogenoideans from Oreochromis niloticus (Hashimoto et al, 2016), and activity against fungi and bacteria (Fabri et al, 2011;Fernandes et al, 2012;Funari et al, 2012;Fontenelle et al, 2007). However, L. sidoides has not been used against monogenoideans from Colossoma macropomum Cuvier, 1818 (tambaqui), an important fish for the fish farming of the region of Amazon.…”

Section: Introductionmentioning

confidence: 99%

Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae)

Soares

Neves²,

Ferreira³

et al. 2017

Veterinary Parasitology

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In vivo and in vitro antiparasitic activity of the essential oil of Lippia sidoides and blood and histological alterations were assessed in Colossoma macropomum (tambaqui). Essential oil concentrations of 10, 20, 40, 80, 160 and 320mg/L were assayed in vitro against monogenoideans Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri from fish gills. Lippia sidoides essential oil concentrations of 320 and 160mg/L were 100% effective against monogenoideans in 10min and 1h of exposure, respectively. However, the effectiveness of 100% concentrations of 80mg/L and 40mg/L occurred in 3 and 6h, respectively. In the in vivo tests, juvenile fish were submitted to 60min of baths with 10mg/L and 15min of baths with 20mg/L of the essential oil of L. sidoides. These therapeutic baths were not efficient against Ichthyophthirius multifiliis, and monogenoideans present in the gills of C. macropomum. In addition, 10 and 20mg/L of the essential oil of L. sidoides caused an anesthetic effect on the fish and did not influence total glucose and protein plasma levels; however, it decreased the number of total erythrocytes in fish exposed to the higher concentration of this essential oil. Severe alterations and irreversible damage were observed in the fish gills just after L. sidoides essential oil baths and after 24h of recovery. The most recurrent lesions found were hyperplasia and fusion of the lamellar epithelium, vasodilation, detachment of the gill epithelium and lamellar aneurism, epithelial breakdown with hemorrhage, congestion, edema and necrosis, proliferation of the mucous cells and chloride cells and lamellar hypertrophy. Therefore, since the essential oil of L. sidoides has in vitro antiparasitic activity and low concentrations of it have shown toxic effects, the bioactive potential of its main chemical components should be investigated, as well as more efficient forms of its administration in therapeutic baths in order to eliminate fish parasites.

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Identification of Antioxidant and Antimicrobial Compounds of Lippia Species by Bioautography

Cited by 27 publications

References 21 publications

Chemical and antifungal investigations of six Lippia species (Verbenaceae) from Brazil

Chemical and antifungal investigations of six Lippia species (Verbenaceae) from Brazil

Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae)

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