Identification of antimony resistance markers in Leishmania tropica field isolates through a cDNA-AFLP approach

Kazemirad, Elham; Mohebali, Mehdi; Khadem-Erfan, Mohammad Bagher; Saffari, Mojtaba; Raoofian, Reza; Hajjaran, Homa; Hadighi, Ramtin; Khamesipour, Ali; Rezaie, Sassan; Abedkhojasteh, Hoda; Heidari, Mansour

doi:10.1016/j.exppara.2013.07.018

Cited by 59 publications

(55 citation statements)

References 46 publications

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“…We had no rapid clinical marker for response to the treatment and were compelled to discontinue Glucantime® after 9th day of fever. It was according to some documented evidence of resistance to Glucantime® against L. infantum and L. tropica in our area [6,7,8,9]. …”

Section: Discussionmentioning

confidence: 62%

Visceral Leishmaniasis without Fever in an 11-Month-Old Infant: a Rare Clinical Feature of Kala-azar

et al. 2014

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Visceral leishmaniasis or kala-azar is an endemic parasitic disease in some parts of the world which is characterized by fever, splenomegaly, and pancytopenia in most of the cases. Herein we report an 11 month-old male infant with diagnosis of kala-azar who presented with pallor, hepatosplenomegaly, failure to gain weight, and no history of fever. Surprisingly, fever started after beginning of meglumine antimoniate treatment in this patient. As far as we are aware of, this is a rare presentation of visceral leishmaniasis. Therefore, clinicians especially in endemic areas are highly recommended to include kala-azar among differential diagnosis of unexplained anemia without fever to prevent misdiagnosis of this potentially fatal, but treatable condition.

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Section: Discussionmentioning

confidence: 62%

Visceral Leishmaniasis without Fever in an 11-Month-Old Infant: a Rare Clinical Feature of Kala-azar

et al. 2014

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“…34 The toxic side effects of anti-leishmanial medications used for treatment and the development of resistance of the parasite to these medications have led to studies related to definition and formulation of new molecules to date. 37 Different studies have been completed; [12][13][14][15][16][17][18] from a study showing the anti-leishmanial activity of ethanol extracts from Allium sativa (garlic) and Azadirachta indica (Neem), locally used for treatment of Cutaneous leishmaniasis in the Sudan, had no significant difference when compared to pentostam, 16 to studies emphasizing that the anti-leishmanial efficacy on promatigotes and amastigotes of Leishmania tropica was higher compared to two antibiotics in the macrolid group of azithromycin and clarithromycin. 38 The AL-EO presented in this study can be considered for use as an antileishmanial.…”

Section: Resultsmentioning

confidence: 99%

“…11,12 Globally and in Turkey, it has been reported that resistance has developed to the primary medication for treatment of CL and VL of five valuable antimony species, sodium stibogluconate (Pentostam®) and megluminantiamoniate (Glucantime®). [12][13][14][15][16] Due to the lack of an effective prophylactic against the disease, the toxic effects of currently-used medications and the increasing resistance to these medications, the necessity for discovery and development of new therapeutic agents has been reported. 11,17,18 Trichomoniosis is a common infection everywhere in the world and the infection rates are reported to show great variations from country to country and society to society.…”

Section: Introductionmentioning

confidence: 99%

Antiparasitic Efficacy of Artemisia ludoviciana Nutt. (Asteraceae) Essential Oil for Acanthamoeba castellanii, Leishmania infantum and Trichomonas vaginalis

Baldemir¹,

Karaman²,

İllgün³

et al. 2018

IJPER

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“…Ayrıca bu ilaçların çoğunun nefrotoksik, hepatotoksik ve teratojenik etkiler başta olmak üzere ciddi yan etkileri bulunmaktadır [3,9,10] . Dünyada ve Türkiye'de KL ve VL'in tedavisinde birincil ilaç olarak kullanılan beş değerli antimon türevleri, sodyum stiboglukonat (Pentostam®) ve megluminantimoniat (Glucantime®) 1940 yılında geliştirilmiş, ancak bu ilaçlara karşı direnç geliştiği, araştırıcılar tarafından bildirilmiştir [6,[11][12][13][14] .…”

Section: Hidrazon Yapısındaki On Farklı Bileşiğin Antileishmanialunclassified

Investigation of Anti-leishmanial Activity of the Ten Different Hydrazone Derivatives

Direkel¹,

Utku²,

Şahi̇n³

et al. 2016

Kafkas Univ Vet Fak Derg

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ÖzetLeishmaniasis Türkiye ve dünyada önemli paraziter bir hastalıktır. Leishmaniasis tedavisinde sodyum stibogluconate, miltefosin, paramomisin, amfoterisin B ve pentamidin gibi anti-leishmanial ilaçlar kullanılmaktadır. Fakat leishmaniasisin kemoterapisinde kullanılan bu ilaçların nefrotoksik, hepatotoksik ve teratojenik yan etkileri görülebilmektedir. Ayrıca antimon bileşiklerine karşı direnç gelişmesi nedeniyle de yeni terapötik ajanların keşfedilmesi ve geliştirilmesine öncelik verilmesi gerektiği düşünülmüştür. Bu çalışmanın amacı, sentezlenmiş hidrazon yapısındaki on farklı bileşiğin (5a-5j) Leishmania infantum promastigotlarına karşı anti-leishmanial aktivitesinin mikrodilüsyon yöntemiyle belirlenmesidir. Hazırlanan hidrazon bileşikleri, konsantrasyonu 6 µg/ml olacak şekilde RPMI-1640 besiyerlerine eklenmiş ve bileşiklerin mikroplakta konsantrasyon aralığı 3 -0.003 µg/ml olacak şekilde seri dilüsyonları yapılmıştır. Mikroplaklarda mikrodilüsyon besiyeri yöntemi uygulanarak, üzerine hemositometrede hücre sayısı 2.5x10 7 hücre/ml olacak şekilde ayarlanmış standart Leishmania infantum promastigotları eklenerek 27°C'de inkübe edilmiştir. Yirmi saat sonra mikroplakların üzerine alamar mavisi eklenerek 4 saat inkübe edilmiştir. Promastigotların üremesi 24, 48 ve 72. saatlerde değerlendirilmiştir. Kuyucuklarda rengin maviden pembeye dönmesi parazitin ürediği, rengin değişmeden kalması ise parazitin üremediği şeklinde değerlendirilmiştir. Bu çalışmada, Leishmania infantum promastigotlarına karşı en etkili maddelerin 5e, 5g ve 5h bileşikleri (MİK 0.187 µg/ml) olduğu, en etkisiz bileşiğin ise 5i bileşiği (MİK 3 µg/ml) olduğu saptanmıştır. Sentezlenen bileşiklerin ilaç olarak kullanılabilmesi için; gerekli olduğu düşünülen in vitro makrofaj kültüründe Leishmania amastigotlarına karşı etkinliği ve in vivo olarak deneysel hayvan modellerinde kontrol çalışmalarına ihtiyaç vardır. Anahtar sözcükler: Leishmania infantum, Hidrazon, Anti-leismanial aktivite, Alamar mavisi Investigation of Anti-leishmanial Activity of the Ten Different Hydrazone Derivatives AbstractLeishmaniasis is an important parasitic disease in Turkey and the world. Anti-leishmanial drugs such as sodium stibogluconate, miltefosine, paramomycin, amphotericin B, and pentamidine are used for the treatment of leishmaniasis. However, the drugs, used for the chemotheraphy of leishmaniasis, have some side effects such as nephrotoxicity, hepatotoxicity, and teratogenicity. In addition, it is deemed that the discovery and development of the new therapeutic agents must be given priority due to the development of resistance against antimony compounds. The purpose of this study is detecting the anti-leishmanial activity of ten different synthesized hydrazone compounds against Leishmania infantum promastigotes via the microdilution method. The prepared hydrazone compounds, having the concentration of 6 µg/ml, were added to RPMI-1640 media and the dilution of the hydrazone derivates was performed in the wells of microplates in the range of concentrations of 3 -0.003 ...

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Identification of antimony resistance markers in Leishmania tropica field isolates through a cDNA-AFLP approach

Cited by 59 publications

References 46 publications

Visceral Leishmaniasis without Fever in an 11-Month-Old Infant: a Rare Clinical Feature of Kala-azar

Visceral Leishmaniasis without Fever in an 11-Month-Old Infant: a Rare Clinical Feature of Kala-azar

Antiparasitic Efficacy of Artemisia ludoviciana Nutt. (Asteraceae) Essential Oil for Acanthamoeba castellanii, Leishmania infantum and Trichomonas vaginalis

Investigation of Anti-leishmanial Activity of the Ten Different Hydrazone Derivatives

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