Identification of antigenic genes in Trichinella spiralis by immunoscreening of cDNA libraries

Proteomic analysis of differentially expressed proteins in the three developmental stages of Trichinella spiralis

“…spiralis has evolved different evasion and suppression mechanisms against these constant pressures and selective forces by expressing different proteins at each stage (Mitreva et al, 2011). Although the expression of stage-specific genes in the helminth has been detected with various immunological, cDNA cloning and RNA sequencing approaches (Liu et al, 2007;Wu et al, 2009;Liu et al, 2012), T. spiralis biology at each developmental stage, including developmental regulation, nutrition metabolism and cell cycle, remains unclear.…”

Screening of early antigen genes of adult-stage Trichinella spiralis using pig serum from different stages of early infection

“…The identified antigens include glutamic acid-rich antigenic proteins, a serine proteinase, a serine protease inhibitor and other novel candidates. Although some antigens have been tested for early diagnosis or for vaccine development, these immunodominant antigens have thus far been insufficient for these purposes (Nagano et al, 2001;Wu et al, 2009;Zarlenga et al, 2002;Zocevic et al, 2011). The complicated life cycle of T. spiralis and the wide variety of stage-specific host immune responses may explain why these antigens have not yielded satisfactory results.…”

Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus

“…The identification and characterization of growth-related genes are the first step in such efforts, and different strategies such as cDNA libraries, homology cloning, and proteomics have been successfully utilized in some previous cases (Wu et al, 2009;Natarajascenivasan et al, 2011;Lenz et al, 2011). Suppression subtractive hybridization (SSH) combines normalization and subtraction in a single procedure, thus allowing the isolation of differentially expressed cDNA that is overexpressed in one population but not in another.…”