Identification of Antifungal Compounds Produced by Lactobacillus casei AST18

Li, Hongjuan; Lü, Ling; Zhang, Shuwen; Cui, Wenming; Lv, Jing

doi:10.1007/s00284-012-0135-2

Cited by 82 publications

(34 citation statements)

References 17 publications

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“…The interactions and mechanisms employed by C. albicans and Lactobacilli remain somewhat enigmatic. Lactobacilli species have demonstrated the ability to inhibit C. albicans growth via the release of hydrogen peroxide and fatty acids [88]. Previous to this, in an ex vivo experiment, L. rhamnosus and L. reuteri altered host responses by eliciting an increased inflammatory cytokine response in a C. albicans coinfection model [89].…”

Section: Denture Stomatitis: Mixing In a Rough Neighbourhoodmentioning

confidence: 99%

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Delaney

Kean

Short

et al. 2018

Curr Clin Micro Rpt

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Purpose of Review Over the last decade, microbiome studies have enhanced our knowledge and understanding of the polymicrobial nature of oral infections. Recently, profiling of the fungal microbiome has expanded our conventional understanding of oral ecology, revealing the critical importance of yeasts within this complex microbiome. This review aims to explore our current appreciation of interkingdom interactions in oral disease. Recent Findings There is a growing evidence base of interactions and pathogenic synergy and antagonism with bacterial species within oral disease. Recent studies have helped to develop our knowledge of how Candida albicans, alongside bacteria such as Porphyromonas gingivalis, Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, and Lactobacillus species, influence overall pathogenicity. Summary Clinical and experimental evidence makes a compelling case for a role for C. albicans in a number of oral infections, though whether its role is an active accomplice or passive bystander remains to be determined.

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Section: Denture Stomatitis: Mixing In a Rough Neighbourhoodmentioning

confidence: 99%

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Delaney

Kean

Short

et al. 2018

Curr Clin Micro Rpt

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“…Prior to HPLC injection, extracts in methanol were filtered through a 0.2 lm PTFE Acrodisc CR syringe filter (Pall Corp., East Hills, NY). Gradient conditions were used with methanol/0.05% trifluoroacetic acid-solvent A (Sigma-Aldrich) and water/ 0.05% trifluoroacetic acid-solvent B at 1 ml/min and A/B ratios of 10:90, 100:0, and 100:0, with run time of 0, 30, and 33 min, respectively (Pryor et al 2006;Li et al 2012). Samples were eluted at a flow rate of 1 ml/min, and the elution profile was monitored with UV detector at 210 nm.…”

Section: Identification Of Antifungal Compoundsmentioning

confidence: 99%

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

2017

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A study was executed in a direction to attenuate Sclerotinia stalk rot (SSR) disease through biocontrol agent and also to enhance crop productivity. Culture filtrate of bacterial strain YSPMK11 inhibited growth of Sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. Interaction studies revealed maximum (81.50%) growth inhibition at 35°C and pH 7.0 after 72 h incubation period with 15% culture filtrate. Based upon 16S rRNA gene sequence strain, YSPMK11 was identified as Bacillus pumilus. Furthermore, the genome of this isolate was searched for antimicrobial lipopeptide, i.e., ItuD and SrfC genes. The PCR amplification results showed the presence of both these lipopeptide genes in isolate YSPMK11. Iturin A as antifungal compound was identified as major components of fraction through GC/MS. In field experiments, the application of strain YSPMK11 cell suspension (10 8 CFU/ml) suppressed disease severity by 93% and increased curd yield by 36% which was more that of commercially used fungicide in farmer practices under mid-hills of Himachal Pradesh.Conclusively, our study is first to demonstrate the effect of B. pumilus strain YSPMK11 in suppression of SSR under field conditions and would be employed as an efficient biocontrol agent to replace commercial fungicides in cauliflower cropping system. In addition, the presence of both lipopeptide genes (ItuD and SrfC) and iturin A in this isolate makes him potent strain for biological control application in agriculture.

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“…Data (Figure 5 A) showed that the highest ( p <0.05) antibacterial activity was observed when pH was 5, excluding the effect of control. This indicated that either organic acids or other pH-dependent antibacterial compounds were responsible for the antibacterial effect [40]. These results indicated that P. orientale leaf extracts would be best used when pH was 5.…”

Section: Discussionmentioning

confidence: 87%

Protective Effect of Polygonum orientale L. Extracts against Clavibater michiganense subsp. sepedonicum, the Causal Agent of Bacterial Ring Rot of Potato

et al. 2013

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The Polygonum orientale L. extracts were investigated for antibacterial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causal agent of a serious disease called bacterial ring rot of potato. The results showed that the leaf extracts of P. orientale had significantly (p<0.05) greater antibacterial activity against C. michiganense subsp. sepedonicum than root, stem, flower extracts in vitro. According to the results of single factor experiments and L273(13) orthogonal experiments, optimum extraction conditions were A1B3C1, extraction time 6 h, temperature 80°C, solid to liquid ratio 1∶10 (g:mL). The highest (p<0.05) antibacterial activity was observed when pH was 5, excluding the effect of control. The extracts were stable under ultraviolet (UV). In vivo analysis revealed that 50 mg/mL of P. orientale leaf extracts was effective in controlling decay. Under field conditions, 50 mg/mL of P. orientale leaf extracts also improved growth parameters (whole plant length, shoot length, root length, plant fresh weight, shoot fresh weight, root fresh weight, dry weight, and number of leaves), in the 2010 and 2011 two growing seasons. Further solvent partition assays showed that the most active compounds were in the petroleum ether fractionation. Transmission electron microscopy (TEM) showed drastic ultrastructural changes caused by petroleum ether fractionation, including bacterial deformation, electron-dense particles, formation of vacuoles and lack of cytoplasmic materials. These results indicated that P. orientale extracts have strong antibacterial activity against C. michiganense subsp. sepedonicum and a promising effect in control of bacterial ring rot of potato disease.

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Identification of Antifungal Compounds Produced by Lactobacillus casei AST18

Cited by 82 publications

References 17 publications

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

Protective Effect of Polygonum orientale L. Extracts against Clavibater michiganense subsp. sepedonicum, the Causal Agent of Bacterial Ring Rot of Potato

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