Identification of Anticancer Compounds in Gallnuts Through PCA-constructed Secondary Metabolite Map

Gao, Jiayu; Yang, Xiao; Hu, Jiangxia; Yin, Weiping

doi:10.3923/ijp.2019.515.522

Cited by 2 publications

(4 citation statements)

References 17 publications

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“…14 Thus, in recent years, MVA has been employed to model phytochemical data and to aid determination of discriminative bioactive constituents. 11,12 In this study, a HPLC-PCA map was constructed to model the metabolic profiling of secondary metabolites and successfully determine the anticancer compounds of Spina Gleditsiae. Compared with the traditional bioassay-guided fractionation, the identification and grouping of functional compounds from plant extracts was much quicker and more efficient through the multivariate analysis mediated secondary metabolite mapping method.…”

Section: Discussionmentioning

confidence: 99%

“…Crude extracts with IC 50 values >186 µg/mL, and 10% ethanol, 25% ethanol and nbutanol extracts are considered as poor The identities of these contribution compounds were thus confirmed by spiking samples with standards isolated and reported previously. 12 This indicated that the variance of growth inhibitory effect of Spina Gleditsiae extracts on HepG2 cancer cells depended on the concentration of these compounds, the chemical structures of which are shown in Figure 2.…”

Section: Hplc-pca Mapmentioning

confidence: 92%

“…The multivariate data analysis (MVA)guided method, developed recently and characterized as minimum consuming of time, raw materials, and solvents, could provide an advanced technique for the fast screening of bioactive agents of complex medicinal products. 11,12 Therefore, a MVA-guided method was employed in this study to identify the anticancer components of Spina Gleditsiae.…”

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confidence: 99%

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Multivariate Data Analysis Guided Identification of Anticancer Constituents of Spina Gleditsiae for Human Hepatoma Cells

Gao

Chen

et al. 2020

Natural Product Communications

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Spina Gleditsia has been traditionally used as a lead ingredient of many formulas for cancer treatment in Traditional Chinese Medicine. This work aimed to reveal the phytochemical foundation of Spina Gleditsiae and underpin a fundamental understanding of its anticancer activities. The growth inhibition activities were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay of 7 solvent extracts of Spina Gleditsia in the human liver cancer cell line HepG2. The activities were then mapped onto the secondary metabolite profile of crude extracts by principal components analysis (PCA) of High Performance Liquid Chromatography-Ultraviolet (HPLC-UV) data. The top 5 PCA components of the map discriminated extract activities, mainly based on the differential content of 3 compounds. The identified compounds were then tested individually using cytotoxic assay on cancer cells in vitro. Principal components analysis constructed secondary metabolite mapping quickly identified functional compounds from the crude herbal extracts. (2 R,3 R)-7,3′,4′-Trihydroxy-5-methoxy-flavanonol, (2 R,3 R)-5,7,3′-trihydroxy-4′-methoxy-flavanonol, and isovitexin inhibited the growth of human liver cancer cell line growth with activities of 38.6 ± 1.9, 40.7 ± 0.9, and 41.5 ± 3.1 μg/mL, respectively, comparable with that of cisplatin (IC50: 41.6 ± 2.9 μg/mL). (2 R,3 R)-7,3′,4′-Trihydroxy-5-methoxy-flavanonol, (2 R,3 R)-5,7,3′-trihydroxy-4′-methoxy-flavanonol, and isovitexin are, for the first time, identified as the anticancer constituents of Spina Gleditsia. It thus strongly underpins a fundamental understanding of the anticancer activities of Spina Gleditsia and supports its ongoing clinical uses in China.

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Section: Discussionmentioning

confidence: 99%

Section: Hplc-pca Mapmentioning

confidence: 92%

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confidence: 99%

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Multivariate Data Analysis Guided Identification of Anticancer Constituents of Spina Gleditsiae for Human Hepatoma Cells

Gao

Chen

et al. 2020

Natural Product Communications

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“…Phenolic acids were mainly detected using UV-visible, DAD, or fluorescence detectors [65]. The HPLC-UV technique was also utilized to detect the com-pounds gallic acid and 1, 2, 3, 4, 6-O-pentagalloyl glucose in a study that aimed to identify anticancer compounds through a PCA-constructed secondary metabolite map in Galla Chinensis and Galla Turcica gallnuts [68].…”

Section: Separation and Characterization Of Phenolic Compounds In Que...mentioning

confidence: 99%

Phytochemical Profiling and Biological Activities of Quercus sp. Galls (Oak Galls): A Systematic Review of Studies Published in the Last 5 Years

Banc,

Rusu,

Filip

et al. 2023

Plants

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Quercus species have been widely used in traditional medicine, and recently, researchers’ attention has focused on galls of the genus Quercus as a source of health-promoting phytochemicals. This review presents a summary of the most recent findings on the phytochemistry and bioactivity of oak galls, following the screening of scientific papers published in two relevant databases, PubMed and Embase, between January 2018 and June 2023. The oak galls are rich in active compounds, mostly gallotannins and phenolic acids. Due to these secondary metabolites, the reviewed studies have demonstrated a wide range of biological activities, including antioxidant and anti-inflammatory actions, antimicrobial properties, tissue-protective effects, and antitumor, anti-aging, and hypoglycemic potential. Thus, oak galls are a promising natural matrix, to be considered in obtaining pharmaceutical and cosmetic preparations used in anti-aging strategies and, together with medications, in the management of age-related diseases. In further evaluations, the valuable functional properties of oak galls, reported mostly in preclinical studies, should be confirmed with clinical studies that would also take into account the potential health risks of their use.

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Identification of Anticancer Compounds in Gallnuts Through PCA-constructed Secondary Metabolite Map

Cited by 2 publications

References 17 publications

Multivariate Data Analysis Guided Identification of Anticancer Constituents of Spina Gleditsiae for Human Hepatoma Cells

Multivariate Data Analysis Guided Identification of Anticancer Constituents of Spina Gleditsiae for Human Hepatoma Cells

Phytochemical Profiling and Biological Activities of Quercus sp. Galls (Oak Galls): A Systematic Review of Studies Published in the Last 5 Years

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