Identification of Antiadhesive Fraction(s) in Nonimmunized Egg Yolk Powder:  In Vitro Study

Kassaify, Zeina; Li, Esther W. Y.; Mine, Yoshinori

doi:10.1021/jf0500031

Cited by 29 publications

(13 citation statements)

References 41 publications

(50 reference statements)

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“…Inhibition of type III secretion by cells treated with nonimmune egg powder may have contributed to the ability of the control egg powder preparation to reduce the adherence of E. coli O157:H7 to HeLa cells. The inhibition of EHEC adherence by control egg powder was not entirely unexpected, as a previous investigation revealed that the high‐density lipoprotein (HDL) component of the granular fraction of chicken egg yolks has a distinct inhibitory effect on the adhesive potential of E. coli O157:H7 (Kassaify et al. 2005).…”

Section: Discussionmentioning

confidence: 93%

Avian- and mammalian-derived antibodies against adherence-associated proteins inhibit host cell colonization byEscherichia coliO157:H7

Cook

Maiti²,

DeVinney

et al. 2007

Journal of Applied Microbiology

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Aim: To evaluate the potential for polyclonal antibodies targeting enterohaemorrhagic Escherichia coli (EHEC) virulence determinants to prevent colonization of host cells by E. coli O157:H7. Methods and Results: Rats and laying hens were immunized with recombinant proteins from E. coli O157:H7, EspA, C‐terminal intimin or EscF. Rat antisera (IgG) or chicken egg powders (IgY) were assessed for their ability to inhibit growth and colonization‐associated processes of E. coli O157:H7. Mammalian antisera with antibodies to intimin, EspA or EscF effectively reduced adherence of the pathogen to HeLa cells (P < 0·05) and prevented type III secretion of Tir. Similarly, HeLa cells treated with chicken egg powder containing antibodies against intimin or EspA were protected from EHEC adherence (P < 0·05). Neither egg nor rat antibody preparations had any antibacterial effect on the growth of EHEC (P > 0·05). Conclusions: Antibody preparations targeting EHEC adherence‐associated factors were effective at preventing adhesion and intimate colonization‐associated events. Significance and Impact of the Study: This work indicates that immunotherapy with anti‐adherence antibodies can reduce E. coli O157:H7 colonization of host cells. Passive immunization with specific antibodies may have the potential to reduce E. coli O157:H7 colonization in hosts such as cattle or humans.

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Section: Discussionmentioning

confidence: 93%

Avian- and mammalian-derived antibodies against adherence-associated proteins inhibit host cell colonization byEscherichia coliO157:H7

Cook

Maiti²,

DeVinney

et al. 2007

Journal of Applied Microbiology

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“…This may be explained by the presence of components such as high‐density lipoproteins (Kassaify and others ) or sialyloligosaccharides and their derivatives (Sugita‐Konishi and others ).…”

Section: Preharvest Methods For Reducing the Risk Of Salmonella Contamentioning

confidence: 99%

Control of Salmonella Contamination of Shell Eggs—Preharvest and Postharvest Methods: A Review

Galis

Marcq

Marlier

et al. 2013

Comp Rev Food Sci Food Safe

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Salmonella Enteritidis is one of the most prevalent foodborne pathogen, its main reservoir being considered the shell egg. As the concerns related to the increasing human salmonellosis cases grow, the need for an application of preventive methods either at the farm level or during the processing steps is crucial for a better control of the foodborne outbreaks due to the consumption of this specific food product. This review focuses on the application of preventive methods at the farm level, on preharvest step, in order to reduce the risk of shell eggs contamination with Salmonella, especially S. Enteritidis, through a better control of the laying hens' infection with this pathogen. As postharvest methods, a 1st approach is the egg storage conditions and the prevention of Salmonella spp. growth and multiplication. In addition, shell eggs may be subjected to eggshell decontamination, to reduce the risk of foodborne outbreaks. Several of these latter mentioned methods are already authorized to be put in place in different countries, as it is the case in the United States of America and Canada. Their efficacy has been proven and their use is regarded by some as mandatory for ensuring shell eggs safety for the consumers.

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“…The authors hypothesized that egg yolk products might have antimicrobial components. It was later found that the fractions conveyed an antiadhesive effect against the food-borne pathogens instead of a direct antimicrobial activity (Kassaify et al 2005). Thus, the fractions itself could either (i) adhere directly to the surface of pathogens, therefore, blocking specific sites required for microbial adherence, or (ii) act indirectly to prevent pathogens' entry by inducing the activation of immune system components responsible of denying access to pathogens into the chicken body from the epithelial surface.…”

mentioning

confidence: 99%

Immunomodulating effects of egg yolk low lipid peptic digests in a murine model

Nelson

Katayama

Mine

et al. 2007

Food and Agricultural Immunology

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There is a close link between nutrition and immunity. Bioactive peptides derived from various protein sources have been demonstrated to exert immunomodulating effects. Those activated sequences may also be found in egg yolk. When administered in vivo to chicken, egg yolk digests prevented bacterial infections. Thus, egg yolk low lipid peptic digests' (EYLLPD) effects were studied in a murine model to elucidate their action's mechanism and immunomodulating capacity. The mucosal immune response was investigated by immunohistochemistry. IL-6 production was evaluated by ELISA. The results show that EYLLPD induces an immune response with an increase in IgA'/ cells, while orchestrating the Th1/Th2 response. Furthermore, they reveal a potential role of the epithelium in the effects of EYLLPD at the epithelial surface, as suggested by the increase of the IL-6 secretion by small intestinal epithelial cells (SIEC). In summary, this study shows that EYLLPD enhances mucosal immune responses and that SIEC might have played an important role in preventing the bacterial infection.

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Identification of Antiadhesive Fraction(s) in Nonimmunized Egg Yolk Powder: In Vitro Study

Cited by 29 publications

References 41 publications

Avian- and mammalian-derived antibodies against adherence-associated proteins inhibit host cell colonization byEscherichia coliO157:H7

Avian- and mammalian-derived antibodies against adherence-associated proteins inhibit host cell colonization byEscherichia coliO157:H7

Control of Salmonella Contamination of Shell Eggs—Preharvest and Postharvest Methods: A Review

Immunomodulating effects of egg yolk low lipid peptic digests in a murine model

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