Identification of anthracnose (Colletotrichum lentis) race 1 resistance loci in lentil by integrating linkage mapping and a genome-wide association study

Abstract

Anthracnose, caused by Colletotrichum lentis, is a devastating disease of lentil in Western Canada. Growing resistant lentil cultivars is the most cost-effective and environmentally friendly approach to prevent seed yield losses that can exceed 70%. To identify loci conferring resistance to anthracnose race 1 in lentil, biparental quantitative trait loci (QTL) mapping of two recombinant inbred line (RIL) populations was integrated with a genome-wide association study (GWAS) using 200 diverse lentil accessions from a lentil diversity panel (LDP). A major-effect QTL (qAnt1.Lc-3) conferring resistance to race 1 was mapped to lentil chromosome 3 and co-located on the lentil physical map for both RIL populations. Clusters of candidate nucleotide binding-leucine-rich repeats (NB-LRR) and other defense-related genes were uncovered within the QTL region. A GWAS detected 14 significant SNP markers associated with race 1 resistance on chromosomes 3, 4, 5, and 6. The most significant GWAS SNPs on chromosome 3 supported qAnt1.Lc-3 and delineated a region of 1.6 Mb containing candidate resistance genes. The identified SNP markers can be directly applied in marker-assisted selection to accelerate the introgression of race 1 resistance in lentil breeding.

QTL Mapping of Seed Fe Concentration in an Interspecific RIL Population Derived fromLens culinaris×Lens ervoides

“…In comparison to cultivated lentil, Lens ervoides has proven to be a good source of resistance genes for various lentil diseases (Tullu et al, 2010;Vail et al, 2012;Podder et al, 2013;Gela et al, 2020, 2021b, Gela, 2021, Adobor et al, 2022. It is important to ascertain how SFeC may vary in these interspecific lentil hybrids and their progenies, which can contribute genetic diversity to cultivated lentil breeding.…”