Identification of Ankrd2, a Novel Skeletal Muscle Gene Coding for a Stretch-Responsive Ankyrin-Repeat Protein

Kemp, Timothy J.; Sadusky, T.J.; Saltisi, F.; Carey, Nessa; Moss, Jill; Yang, Shiyu; Sassoon, David; Goldspink, Geoffrey; Coulton, Gary R.

doi:10.1006/geno.2000.6213

Cited by 116 publications

(122 citation statements)

References 55 publications

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“…These spots were not detected in Hanwoo beef and were identified as ankyrin repeat domain 2 (Arpp2) and creatin kinase (CK) isomers by LC-MS/MS analysis (Table 1). Arpp2 on spot 9 was reported to be found in striated muscle and in response to muscle stretch (Kemp et al, 2000;Miller et al, 2003). Ohsaki et al (2007) observed that very high levels of Arpp2 proteins were only expressed in Holstein beef, similar to our results.…”

Section: Discussionsupporting

confidence: 90%

Proteomics Comparison of Longissimus Muscle between Hanwoo and Holstein Cattle

Shim¹,

Park²,

Hwang³

et al. 2010

Korean Journal for Food Science of Animal Resources

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This study was conducted to compare proteins expressed in M. longissimus from Hanwoo and Holstein steers immediately after slaughter. Two-dimensional electrophoresis (2DE)/LC-MS/MS analysis revealed that the total number of detectable protein spots from longissimus muscle tissues was slightly higher in Hanwoo (575 ± 65) than Holstein (534 ± 13) steers, but that these numbers were not statistically significant due to large variation between replicates. A total of twelve protein spots did not match between sample groups, eight of which were expressed in the Hanwoo sample and four that were expressed in the Holstein sample. The protein spots detected in the Hanwoo sample included smooth muscle and non-muscle myosin alkali light chain 6B isomers, αB crystallin isomers, hemoglobin β-A chains, slow myosin heavy chains, and slow skeletal muscle troponin T chains. Collectively, these proteins are a class of slow-twitch muscle fiber and mirror that Hanwoo muscle tissue sampled for the current study contained more slow-twitch muscle fibers than Holstein one. Conversely, proteins detected from the Holstein sample included ankyrin repeat domain 2 and creatin kinase isomers. Given that creatin kinase isomers are related to the fast-twitch muscle, these results likely indicate that Holstein muscle tissue sampled for the current study contained more fast-twitch muscle fibers than Hanwoo beef.

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Section: Discussionsupporting

confidence: 90%

Proteomics Comparison of Longissimus Muscle between Hanwoo and Holstein Cattle

Shim¹,

Park²,

Hwang³

et al. 2010

Korean Journal for Food Science of Animal Resources

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“…Analysis using the DDBJ revealed that the cloned cDNA is almost identical to Arkrd2 cDNA, which was isolated as a mouse gene induced in stretched skeletal muscle (Kemp et al, 2000). Although the Ankrd2 gene was expected to encode an ankyrin-repeat protein composed of 328 amino acids (Kemp et al, 2000), in the present study mArpp was found to contain 332 amino acids.…”

Section: Discussionmentioning

confidence: 54%

“…Although the Ankrd2 gene was expected to encode an ankyrin-repeat protein composed of 328 amino acids (Kemp et al, 2000), in the present study mArpp was found to contain 332 amino acids. This discrepancy may be explained by the fact that the reported cDNA of Ankrd2 did not cover the full-length open reading frame and lacked the first methionine.…”

Section: Discussionmentioning

confidence: 54%

“…Comparison of the mouse Arpp (mArpp) gene with sequences in the DNA Data Bank of Japan (DDBJ) revealed that the mArpp gene was identical to the recently isolated mouse Ankrd2 gene, which is induced in stretched skeletal muscle (Kemp et al, 2000). Furthermore, we found that mArpp was preferentially expressed in type 1 skeletal muscle fibers, but it was not expressed in heart.…”

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confidence: 52%

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Arpp, a New Homolog of Carp, Is Preferentially Expressed in Type 1 Skeletal Muscle Fibers and Is Markedly Induced by Denervation

Tsukamoto

Senda

Nakano

et al. 2002

Laboratory Investigation

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SUMMARY:In this study, we isolated and characterized a murine counterpart of the human Arpp (hArpp) gene. Sequence analysis revealed that the murine Arpp (mArpp) gene is almost identical to the Ankrd2 gene, which has recently been isolated as a mouse gene induced in stretched skeletal muscle. The mArpp gene encodes a protein of 332 amino acids that contains four well-conserved ankyrin-repeat domains in the central portion of the protein. The amino acid sequence of mArpp protein (mArpp) is highly homologous to that of mouse cardiac-restricted ankyrin-repeat protein (Carp), which is proposed to be a putative genetic marker for cardiac hypertrophy. Immunohistochemical analysis revealed that mArpp is preferentially expressed in type 1 skeletal muscle fibers, and that mArpp is localized in both the nucleus and the sarcomeric I-band of muscle fibers, suggesting that Arpp may function as a nuclear and sarcomeric protein. Furthermore, mArpp was also expressed in neurons of the cerebellum and cerebrum, the islets of Langerhans in the pancreas, and the esophageal epithelium, suggesting that mArpp may play a functional physiologic role in brain, pancreas, and esophagus as well as in type 1 muscle fibers. Interestingly, although mArpp was localized in both nucleus and cytoplasm in neurons, its localization was restricted to nucleus in pancreas and esophagus, suggesting that intracellular localization of mArpp is regulated in a tissue-specific manner. Furthermore, we found that mArpp-and Carpexpression in skeletal muscle were markedly up-regulated after denervation. Although the elevated expression level of Carp was kept only for two weeks after denervation, that of Arpp was kept at least for 4 weeks, suggesting that mArpp and Carp may play distinct functional roles in denervated skeletal muscle. (Lab Invest 2002, 82:645-655).

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“…(values of 1.4-1.9 were considered satisfactory). RRT-PCR was performed as described previously [26]. Primers were designed (forward primer, 5h-ATGACTGCAAAGATGGAAACG-3h ; reverse primer, 5h-TATTCTGGCTATGCAGTTCAG-3h) from the published rat c-jun sequence (EMBL accession number X17215 [8] …”

Section: Ratiometric Reverse Transcriptase Pcr (Rrt-pcr)mentioning

confidence: 99%

Up-regulation of c-jun mRNA in cardiac myocytes requires the extracellular signal-regulated kinase cascade, but c-Jun N-terminal kinases are required for efficient up-regulation of c-Jun protein

Clerk

Kemp

Harrison

et al. 2002

Biochemical Journal

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Cardiac hypertrophy, an important adaptational response, is associated with up-regulation of the immediate early gene, c-jun, which encodes the c-Jun transcription factor. c-Jun may feed back to up-regulate its own transcription and, since the c-Jun N-terminal kinase (JNK) family of mitogen-activated protein kinases (MAPKs) phosphorylate c-Jun(Ser-63/73) to increase its transactivating activity, JNKs are thought to be the principal factors involved in c-jun up-regulation. Hypertrophy in primary cultures of cardiac myocytes is induced by endothelin-1, phenylephrine or PMA, probably through activation of one or more of the MAPK family. These three agonists increased c-jun mRNA with the rank order of potency of PMAendothelin-1> phenylephrine. Up-regulation of c-jun mRNA by endothelin-1 was attenuated by inhibitors of protein kinase C (GF109203X) and the extracellular signal-regulated kinase (ERK) cascade (PD98059 or U0126), but not by inhibitors of the JNK (SP600125) or p38-MAPK (SB203580) cascades. Hyperosmotic shock (0.5M sorbitol) powerfully activates JNKs, but did not increase c-jun mRNA. These data suggest that ERKs, rather than JNKs, are required for c-jun up-regulation. However, endothelin-1 and phenylephrine induced greater up-regulation of c-Jun protein than PMA and phosphorylation of c-Jun(Ser-63/73) correlated with the level of c-Jun protein. Up-regulation of c-Jun protein by endothelin-1 was attenuated by inhibitors of protein kinase C and the ERK cascade, probably correlating with a primary input of ERKs into transcription. In addition, SP600125 inhibited the phosphorylation of c-Jun(Ser-63/73), attenuated the increase in c-Jun protein induced by endothelin-1 and increased the rate of c-Jun degradation. Thus whereas ERKs are the principal MAPKs required for c-jun transcription, JNKs are necessary to stabilize c-Jun for efficient up-regulation of the protein.

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Identification of Ankrd2, a Novel Skeletal Muscle Gene Coding for a Stretch-Responsive Ankyrin-Repeat Protein

Cited by 116 publications

References 55 publications

Proteomics Comparison of Longissimus Muscle between Hanwoo and Holstein Cattle

Proteomics Comparison of Longissimus Muscle between Hanwoo and Holstein Cattle

Arpp, a New Homolog of Carp, Is Preferentially Expressed in Type 1 Skeletal Muscle Fibers and Is Markedly Induced by Denervation

Up-regulation of c-jun mRNA in cardiac myocytes requires the extracellular signal-regulated kinase cascade, but c-Jun N-terminal kinases are required for efficient up-regulation of c-Jun protein

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