Identification of animal species by protein radioimmunoassay of bone fragments and bloodstained stone tools

Lowenstein, Jerold M.; Reuther, Joshua D.; Hood, Darden; Scheuenstuhl, Gary; Gerlach, S. Craig; Ubelaker, Douglas H.

doi:10.1016/j.forsciint.2005.08.007

Cited by 41 publications

(22 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several alternative approaches to species identification of fragmentary bones have been considered, ranging from histology (Hars anyi 1993;Hillier & Bell 2007;Cuijpers & Lauwerier 2008;Greenlee & Dunnell 2010), to biomolecular analyses (Lowenstein et al 2006;Blow et al 2008). Histological analysis, which can be considered a type of morphological analysis on a microscopic scale, is relatively low cost but has not been adopted widely in archaeology or palaeontology due to the amount of time, expense and technical expertise required in relation to the limited taxonomic resolution available (Cuijpers 2006).…”

mentioning

confidence: 99%

Species identification and decay assessment of Late Pleistocene fragmentary vertebrate remains from Pin Hole Cave (Creswell Crags, UK) using collagen fingerprinting

Buckley

Harvey

Chamberlain

2017

Boreas

View full text Add to dashboard Cite

Ancient bone remains are widely utilized when investigating vertebrate biodiversity of past animal populations but are often so highly fragmented that the majority of specimens cannot be identified to any meaningful taxonomic level. Recently, high‐throughput methods for objective species identification using collagen peptide mass fingerprinting have been created to overcome this with the added indication that they could also offer a means of relative ageing through decay measurement. Here we explore both species identification and decay measurements for the Pin Hole Cave ‘microfaunal’ assemblage, the site that has been designated as a representative for Marine Oxygen Isotope Stage 3 in Britain in terms of its suite of mammalian fauna. We explore the technique's potential to corroborate the faunal diversity established previously by macroscopic studies and evaluate the decay measurements across the species boundary. The results support that the analysis of fragmentary remains by collagen fingerprinting can yield a more diverse set of fauna, and offer additional information relating to taphonomy, than the analysis of morphologically intact bones on their own. However, although useful for identifying likely contaminations of an assemblage, there was an unexpected decrease in the decay measurements observed for some megafauna compared with much younger microfauna, indicating that other factors need to be carefully monitored before it could be used as a relative ageing technique in Quaternary deposits.

show abstract

mentioning

confidence: 99%

Species identification and decay assessment of Late Pleistocene fragmentary vertebrate remains from Pin Hole Cave (Creswell Crags, UK) using collagen fingerprinting

Buckley

Harvey

Chamberlain

2017

Boreas

View full text Add to dashboard Cite

show abstract

“…Highest percentage of binding reflects a significant reaction and is interpreted as an identification of a species. Non-identification (''no reaction'') occurs when values are extremely low; we consider significant reactions to be !3 (following earlier studies, e.g., Lowenstein et al, 2006). Weak reactions can occur when one of the three test runs yields significant high binding values and subsequent testing yields little to no reaction values.…”

Section: Pria Techniquementioning

confidence: 70%

Assessing the reliability of pRIA for identifying ancient proteins from archaeological contexts

Potter

Reuther

Lowenstein

et al. 2010

Journal of Archaeological Science

Self Cite

View full text Add to dashboard Cite

“…The protein-based methods rely on immune assays or chromatography that includes gas chromatography (GC) [6], liquid chromatography (LC) [25], high-performance liquid chromatography (HPLC) [5], enzyme-linked immunosorbent assay (ELISA) [26], several blotting immunological assays [27,28], and electrophoretic analysis [29,30]. However, each technique has its own limitations, such as being expensive, laborious, inadequate, and time-consuming, diverse range of equipment, and difficulty to the interpretation of obtained results [10].…”

Section: Introductionmentioning

confidence: 99%

Rapid Identification of Pork Adulterated in the Beef and Mutton by Infrared Spectroscopy

Yang

Liu

et al. 2018

Journal of Spectroscopy

View full text Add to dashboard Cite

Consumers concern about food adulteration. Pork meat is the principal adulterated species of beef and mutton. The conventional detection methods have their own limitations; therefore, we sought to develop an efficient and economical identification method using an infrared spectroscopy technique for meat. The Mahalanobis distance method was used to remove outliers in spectrum data. Interferences were eliminated using multiple scatter correction, standard normal variate, Savitzky-Golay smoothing, and normalization. The partial least square discriminant analysis (PLS-DA) and support vector machine (SVM) were used to establish identification models. In the Mahalanobis distance method, the coefficient of test sets was increased from 0.93 to 0.99; the RMSEC and RMSECV were decreased from 0.17 to 0.09 and 0.21 to 0.11 accordingly. The coefficient of determination in-between the calibration and testing sets in PLS-DA reached 0.99 and 0.99, RMSEC was 0.06, and both the RMSECV and RMSEP were 0.08. In contrast, in SVM, methods were 0.97 and 0.96. The RMSEC, RMSECV, and RMSEP were 0.15, 0.17, and 0.24, respectively. In summary, using a combination of infrared spectroscopy technology with PLS-DA was a better identification method than the SVM method that can be used as an effective method to identify pork, beef, and mutton meat samples.

show abstract

Identification of animal species by protein radioimmunoassay of bone fragments and bloodstained stone tools

Cited by 41 publications

References 14 publications

Species identification and decay assessment of Late Pleistocene fragmentary vertebrate remains from Pin Hole Cave (Creswell Crags, UK) using collagen fingerprinting

Species identification and decay assessment of Late Pleistocene fragmentary vertebrate remains from Pin Hole Cave (Creswell Crags, UK) using collagen fingerprinting

Assessing the reliability of pRIA for identifying ancient proteins from archaeological contexts

Rapid Identification of Pork Adulterated in the Beef and Mutton by Infrared Spectroscopy

Contact Info

Product

Resources

About