Identification of Angiotensin I in a Cyclostome, Lampetra fluviatilis

Rankin, J. C.; Watanabe, Takushi X.; Nakajima, Kiichiro; Broadhead, Caren; Takei, Yoshiyuki

doi:10.2108/zsj.21.173

Cited by 26 publications

(11 citation statements)

References 24 publications

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“…Once anaesthetised, lampreys were weighed and placed on their backs in individual Perspex troughs with their heads immersed in aerated water containing anaesthetic and maintained at 10°C; the rest of the body was covered with damp tissue. Respiratory movements were monitored, and anaesthesia adjusted to maintain strong ventilation and stable blood pressure and renal function (McVicar and Rankin, 1983;Brown and Rankin, 1999;Rankin et al, 2004). Blood samples were collected by needle puncture of the caudal vein.…”

Section: Methodsmentioning

confidence: 99%

Activation of the newly discovered cyclostome renin–angiotensin system in the river lampreyLampetra fluviatilis

Brown

Cobb

Frankling

et al. 2005

Journal of Experimental Biology

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SUMMARY This study describes the first investigations of the physiological signals involved in activating the newly discovered cyclostome renin–angiotensin system (RAS) and its role in the river lamprey Lampetra fluviatilis. Experimental manipulation showed that volume depletion (removal of 40% blood volume) rapidly activated the RAS of lampreys acclimated to water at 576 mOsm kg–1 (21 p.p.t.), significantly increasing plasma angiotensin concentrations after 30 min and 60 min. In agreement with these results, a rapid change in environmental salinity (758 mOsm kg–1 to freshwater (FW) and FW to 605 mOsm kg–1), resulted in a rapid decrease and increase in plasma [angiotensin], respectively. Intraperitoneal(i.p.) injection of FW-acclimated river lampreys with 1% body mass by volume of nominally isosmotic saline (120 mmol l–1 NaCl; 233 mOsm kg–1) resulted in a significant decrease in the plasma angiotensin concentration within 15 min. In contrast, i.p. injection of hyperosmotic saline (4 mol l–1 NaCl) at 1% body mass by volume, which significantly increased plasma osmolality, had no significant effect on plasma [angiotensin], suggesting that volume/pressure receptors and osmoreceptors interact in regulating the lamprey RAS. These results indicate an important role for volume/pressor receptors, as in teleosts, but with an additional osmoreceptor mechanism, such that circulatory [angiotensin] is determined by interaction of volume/pressure and osmoreceptors and their relative sensitivities. The volume/pressure sensitivity is in keeping with the recent evidence of a vasoconstrictor action of homologous lamprey angiotensin and provides evidence that the fundamental role of the RAS in maintaining volume and pressure is an ancient function conserved over 500 million years of vertebrate evolution.

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Section: Methodsmentioning

confidence: 99%

Activation of the newly discovered cyclostome renin–angiotensin system in the river lampreyLampetra fluviatilis

Brown

Cobb

Frankling

et al. 2005

Journal of Experimental Biology

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“…Missing from the authors' data are mass-spectrometry-determined amino acid sequences of the peptides. These results are fascinating; however, more precise methodologies would be important to prove beyond any doubt that the investigators indeed were dealing with Ang II [19]. …”

Section: Evolution Of the Renin–angiotensin Systemmentioning

confidence: 99%

Emergence and evolution of the renin–angiotensin–aldosterone system

et al. 2012

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The renin–angiotensin–aldosterone system (RAAS) is not the sole, but perhaps the most important volume regulator in vertebrates. To gain insights into the function and evolution of its components, we conducted a phylogenetic analysis of its main related genes. We found that important parts of the system began to appear with primitive chordates and tunicates and that all major components were present at the divergence of bony fish, with the exception of the Mas receptor. The Mas receptor first appears after the bony-fish/tetrapod divergence. This phase of evolutionary innovation happened about 400 million years ago. We found solid evidence that angiotensinogen made its appearance in cartilage fish. The presence of several RAAS genes in organisms that lack all the components shows that these genes have had other ancestral functions outside of their current role. Our analysis underscores the utility of sequence comparisons in the study of evolution. Such analyses may provide new hypotheses as to how and why in today's population an increased activity of the RAAS frequently leads to faulty salt and volume regulation, hypertension, and cardiovascular diseases, opening up new and clinically important research areas for evolutionary medicine.Electronic supplementary materialThe online version of this article (doi:10.1007/s00109-012-0894-z) contains supplementary material, which is available to authorized users.

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“…Considerable amounts of data have demonstrated that, in addition to the two main effector peptides of the brain RAS, ANG II and ANG III, ANG IV, and to a lesser extend ANG 1-7 are implicated in various physiological functions notably body fluid homeostasis and blood pressure regulation but also pituitary hormone release, thermoregulation, sexual, eating and locomotor behaviors, metabolism, learning, and memory (28,29,35,36,42,54). Compared with the central actions of the RAS on cardiovascular regulations, studies on the ventilatory effect are sparse and only the action of ANG II has been explored with conflicting results in mammals (13,31,33,37,41,50).The RAS has an ancient evolutionary history and most of its components are present in lampreys, elasmobranchs, and teleosts (29,32,38,46,53). In contrast to the well-known peripheral cardiovascular and osmoregulatory effects of ANG II (5,32,45), studies on the central action are sparse in fishes.…”

mentioning

confidence: 99%

“…The RAS has an ancient evolutionary history and most of its components are present in lampreys, elasmobranchs, and teleosts (29,32,38,46,53). In contrast to the well-known peripheral cardiovascular and osmoregulatory effects of ANG II (5,32,45), studies on the central action are sparse in fishes.…”

mentioning

confidence: 99%

Central ventilatory and cardiovascular actions of angiotensin peptides in trout

Lancien

Wong

Arab

et al. 2012

American Journal of Physiology-Regulatory, Integrative and Comp

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In the brains of teleosts, angiotensin II (ANG II), one of the main effector peptides of the renin-angiotensin system, is implicated in various physiological functions notably body fluid and electrolyte homeostasis and cardiovascular regulation, but nothing is known regarding the potential action of ANG II and other angiotensin derivatives on ventilation. Consequently, the goal of the present study was to determine possible ventilatory and cardiovascular effects of intracerebroventricular injection of picomole doses (5-100 pmol) of trout [Asn(1)]-ANG II, [Asp(1)]-ANG II, ANG III, ANG IV, and ANG 1-7 into the third ventricle of unanesthetized trout. The central actions of these peptides were also compared with their ventilatory and cardiovascular actions when injected peripherally. Finally, we examined the presence of [Asn(1)]-ANG II, [Asp(1)]-ANG II, ANG III, and ANG IV in the brain and plasma using radioimmunoassay coupled with high-performance liquid chromatography. After intracerebroventricular injection, [Asn(1)]-ANG II and [Asp(1)]-ANG II two ANG IIs, elevated the total ventilation through a selective stimulatory action on the ventilation amplitude. However, the hyperventilatory effect of [Asn(1)]-ANG II was threefold higher than the effect of [Asp(1)]-ANG II at the 50-pmol dose. ANG III, ANG IV, and ANG 1-7 were without effect. In addition, ANG IIs and ANG III increased dorsal aortic blood pressure (P(DA)) and heart rate (HR). After intra-arterial injections, none of the ANG II peptides affected the ventilation but [Asn(1)]-ANG II, [Asp(1)]-ANG II, and ANG III elevated P(DA) (50 pmol: +80%, +58% and +48%, respectively) without significant decrease in HR. In brain tissue, comparable amounts of [Asn(1)]-ANG II and [Asp(1)]-ANG II were detected (ca. 40 fmol/mg brain tissue), but ANG III was not detected, and the amount of ANG IV was about eightfold lower than the content of the ANG IIs. In plasma, ANG IIs were also the major angiotensins (ca. 110 fmol/ml plasma), while significant but lower amounts of ANG III and ANG IV were present in plasma. In conclusion, our study suggests that the two ANG II isoforms produced within the brain may act as a neurotransmitter and/or neuromodulator to regulate the cardioventilatory functions in trout. In the periphery, two ANG IIs and their COOH-terminal peptides may act as a circulating hormone preferentially involved in cardiovascular regulations.

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Identification of Angiotensin I in a Cyclostome, Lampetra fluviatilis

Cited by 26 publications

References 24 publications

Activation of the newly discovered cyclostome renin–angiotensin system in the river lampreyLampetra fluviatilis

Activation of the newly discovered cyclostome renin–angiotensin system in the river lampreyLampetra fluviatilis

Emergence and evolution of the renin–angiotensin–aldosterone system

Central ventilatory and cardiovascular actions of angiotensin peptides in trout

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