Identification of an lncRNA-miRNA-mRNA interaction mechanism in breast cancer based on bioinformatic analysis

Abstract

Non-coding RNAs serve important roles in regulating the expression of certain genes and are involved in the principal biological processes of breast cancer. The majority of studies have focused on defining the regulatory functions of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs/miRs), and few studies have investigated how lncRNAs and miRNAs are transcriptionally regulated. In the present study, based on the breast invasive carcinoma dataset from The Cancer Genome Atlas at cBioPortal, and using a bioinformatics computational approach, an lncRNA-miRNA-mRNA network was constructed. The network consisted of 601 nodes and 706 edges, which represented the complex web of regulatory effects between lncRNAs, miRNAs and target genes. The results of the present study demonstrated that miR-510 was the most potent miRNA controller and regulator of numerous target genes. In addition, it was observed that the lncRNAs PVT1, CCAT1 and linc00861 exhibited possible interactions with clinical biomarkers, including receptor tyrosine-protein kinase erbB-2, estrogen receptor and progesterone receptor, demonstrated using RNA-protein interaction prediction software. The network of lncRNA-miRNA-mRNA interactions will facilitate further experimental studies and may be used to refine biomarker predictions for developing novel therapeutic approaches in breast cancer.

Analysis of long non-coding RNAs in neonatal piglets at different stages of porcine deltacoronavirus infection

“…Interestingly, both lncRNA and mRNA in the ceRNA network were negatively correlated with miRNA. Previous studies have confirmed that ceRNAs can act as miRNA 'sponges' and this is especially important for cancer and tumor diseases [27,28]. By analyzing the miRNAs in the ceRNA network we found that the target miRNAs ssc-miR-885-5p, ssc-miR-490-5p, ssc-miR-196b-5p and ssc-miR-133b of lncRNAs were all up regulated.…”

Retracted: Knockdown of long noncoding RNA urothelial carcinoma–associated 1 inhibits cell viability, migration, and invasion by regulating microRNA‐182 in gastric carcinoma

“…24,25 One study reported that UCA1 silencing impaired lung cancer cell proliferation and colony formation by regulation of miR-193a-3p. 24,25 One study reported that UCA1 silencing impaired lung cancer cell proliferation and colony formation by regulation of miR-193a-3p.…”

LncRNA MEG3 inhibits rheumatoid arthritis through miR‐141 and inactivation of AKT/mTOR signalling pathway

“…12,13 For example, in the collagen, antibody induced arthritis mouse model of RA, shikonin can suppress the inflammation by targeting specific lncRNAs, 14 and in presence of quercetin, the apoptosis of fibroblast-like synoviocytes (FLS) in RA was also blocked, in which the involvement of lncRNA MALAT1 plays a critical role. [15][16][17][18][19][20][21][22] In this study, we attempted to discover how MEG3 affects the proliferation and inflammation of LPS-treated chondrocytes in RA.…”