Identification of an IgD/IgT chimera in the European sea bass (Dicentrarchus labrax L.)

Buonocore, Francesco; Scapigliati, Giuseppe; Pallavicini, Alberto; Gerdol, Marco

doi:10.1016/j.fsi.2020.07.041

Cited by 10 publications

(2 citation statements)

References 25 publications

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“…The authors emphasize that this is the product of a genomic recombination event between the Cδ and Cτ genes after duplication of the IgH locus, with a 1/2/3/4/5/6/3/4 exon organization. Also underlining that the expression in different tissues of this chimaera is greater than that of IgD and, specifically in the skin (also greater than that of IgT) (Buonocore et al, 2020). Thanks to the use of CHfinder we were able to explore the possibility that this chimerism was present in other fish species.…”

Section: Immunoglobulin D In Non-teleost Ray Finned Fishesmentioning

confidence: 99%

Tracing the origin of fish immunoglobulins

Gambón-Deza

Mirete-Bachiller

2022

Preprint

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We have studied the origin of immunoglobulin genes in fish. There are two evolutionary lines of bony fish, Actinopterygii and Sarcopterygii. The former gave rise to most of the current fish and the latter to the animals that went to land. Non-teleost actinopterygians are significant evolutionary, sharing a common ancestor with sarcopterygians. There are three different immunoglobulin isotypes in ray-finned fish: IgM, IgD and IgT. We deduce that translocon formation in immunoglobulins genes occurred already in non-teleost Actinopterygii. We establish a relationship between non-teleosts and teleostean fish at the domain level of different immunoglobulins. We found two evolutionary lines of immunoglobulin. A line that starts from Immunoglobulin M and another from an ancestral Immunoglobulin W. The M line is stable, and the W line gives rise to the IgD of the fish. Immunoglobulin T emerges by recombination between both lines.

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Section: Immunoglobulin D In Non-teleost Ray Finned Fishesmentioning

confidence: 99%

Tracing the origin of fish immunoglobulins

Gambón-Deza

Mirete-Bachiller

2022

Preprint

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“…Most IgT genes have four Cτ exons irrespectively if they are membrane bound or secreted [ 16 ]. Notably, chimeric IgD/IgT and IgM/IgT receptors were found in European sea bass [ 17 ] and the common carp [ 18 ], respectively. There is a considerable variation in the number of Ig genes and loci in teleosts.…”

Section: Introductionmentioning

confidence: 99%

Systematic characterization of immunoglobulin loci and deep sequencing of the expressed repertoire in the Atlantic cod (Gadus morhua)

Györkei,

Johansen,

Qiao

2024

BMC Genomics

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Background The Atlantic cod is a prolific species in the Atlantic, despite its inconsistent specific antibody response. It presents a peculiar case within vertebrate immunology due to its distinct immune system, characterized by the absence of MHCII antigen presentation pathway, required for T cell-dependent antibody responses. Thorough characterisation of immunoglobulin loci and analysis of the antibody repertoire is necessary to further our understanding of the Atlantic cod’s immune response on a molecular level. Results A comprehensive search of the cod genome (gadmor3.0) identified the complete set of IgH genes organized into three sequential translocons on chromosome 2, while IgL genes were located on chromosomes 2 and 5. The Atlantic cod displayed a moderate germline V gene diversity, comprising four V gene families for both IgH and IgL, each with distinct chromosomal locations and organizational structures. 5’RACE sequencing revealed a diverse range of heavy chain CDR3 sequences and relatively limited CDR3 diversity in light chains. The analysis highlighted a differential impact of V-gene germline CDR3 length on receptor CDR3 length between heavy and light chains, underlining different recombination processes. Conclusions This study reveals that the Atlantic cod, despite its inconsistent antibody response, maintains a level of immunoglobulin diversity comparable to other fish species. The findings suggest that the extensive recent duplications of kappa light chain genes do not result in increased repertoire diversity. This research provides a comprehensive view of the Atlantic cod's immunoglobulin gene organization and repertoire, necessary for future studies of antibody responses at the molecular level.

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