Identification of anN‐acetylglucosamine kinase essential for UDP‐N‐acetylglucosamine salvage synthesis inArabidopsis

Furo, Keisuke; Nozaki, Mamoru; Murashige, Hiroshi; Sato, Yasushi

doi:10.1016/j.febslet.2015.09.011

Cited by 21 publications

(22 citation statements)

References 20 publications

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“…Abbreviations of seasons: Oct, October; Feb, February; Apr, April; Jun, June. The pathways were designed based on Kegg pathways ( http://www.genome.jp/kegg/pathway.html?sess=2764b8338258d6286de91bbebe6faf46 ) and on Hirabayashi and Ichikawa ( 2002 ), Gardocki et al ( 2005 ), Li-Beisson et al ( 2010 ), Boudière et al ( 2014 ), Guschina et al ( 2014 ), Okazaki et al ( 2013 , 2015 ), Furo et al ( 2015 ), Kobayashi ( 2016 ), and Galili et al ( 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Seasonal Alterations in Organic Phosphorus Metabolism Drive the Phosphorus Economy of Annual Growth in F. sylvatica Trees on P-Impoverished Soil

et al. 2018

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Phosphorus (P) is one of the most important macronutrients limiting plant growth and development, particularly in forest ecosystems such as temperate beech (Fagus sylvatica) forests in Central Europe. Efficient tree internal P cycling during annual growth is an important strategy of beech trees to adapt to low soil-P. Organic P (Porg) is thought to play a decisive role in P cycling, but the significance of individual compounds and processes has not been elucidated. To identify processes and metabolites involved in P cycling of beech trees, polar-metabolome and lipidome profiling was performed during annual growth with twig tissues from a sufficient (Conventwald, Con) and a low-soil-P (Tuttlingen, Tut) forest. Autumnal phospholipid degradation in leaves and P export from senescent leaves, accumulation of phospholipids and glucosamine-6-phosphate (GlcN6P) in the bark, storage of N-acetyl-D-glucosamine-6-phosphate (GlcNAc6P) in the wood, and establishing of a phospholipid “start-up capital” in buds constitute main processes involved in P cycling that were enhanced in beech trees on low-P soil of the Tut forest. In spring, mobilization of P from storage pools in the bark contributed to an effective P cycling. Due to the higher phospholipid “start-up capital” in buds of Tut beeches, the P metabolite profile in developing leaves in spring was similar in beech trees of both forests. During summer, leaves of Tut beeches meet their phosphate (Pi) needs by replacing phospholipids by galacto- and sulfolipids. Thus, several processes contribute to adequate Pi supply on P impoverished soil thereby mediating similar growth of beech at low and sufficient soil-P availability.

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Section: Resultsmentioning

confidence: 99%

Seasonal Alterations in Organic Phosphorus Metabolism Drive the Phosphorus Economy of Annual Growth in F. sylvatica Trees on P-Impoverished Soil

et al. 2018

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“…[33] The growth defects could be suppressed by adding exogenous GlcNAc.T he presence of aG lcNAc salvage pathway in Arabidopsis was further supported by identification of Arabidopsis GlcNAc kinase (GNK), an essential enzyme for the salvage of GlcNAc. [34] Therefore,w er easoned that the GlcNAc salvage pathway in Arabidopsis might be exploited to convey GlcNAz into the N-linked glycans (Figure 1b).…”

Section: Metabolic Labeling and Imaging Of N-linked Glycans In Arabidmentioning

confidence: 99%

Metabolic Labeling and Imaging of N‐Linked Glycans in Arabidopsis Thaliana

Zhu

Chen

2016

Angew Chem Int Ed

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Molecular imaging of glycans has been actively pursued in animal systems for the past decades. However, visualization of plant glycans remains underdeveloped, despite that glycosylation is essential for the life cycle of plants. Metabolic glycan labeling in Arabidopsis thaliana by using N-azidoacetylglucosamine (GlcNAz) as the chemical reporter is reported. GlcNAz is metabolized through the salvage pathway of N-acetylglucosamine (GlcNAc) and incorporated into N-linked glycans, and possibly intracellular O-GlcNAc. Click-labeling with fluorescent probes enables visualization of newly synthesized N-linked glycans. N-glycosylation in the root tissue was discovered to possess distinct distribution patterns in different developmental zones, suggesting that N-glycosylation is regulated in a developmental stage-dependent manner. This work shows the utility of metabolic glycan labeling in elucidating the function of N-linked glycosylation in plants.

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“…As Arabidopsis does not synthesize GalNAc‐containing glycans, GalNAc was probably converted into UDP‐GlcNAc. However, the interconversion pathway is not completely understood . The alkynyl analogues of GlcNAc and GalNAc did not show significant metabolic labeling.…”

Section: Resultsmentioning

confidence: 98%

Expanding the Scope of Metabolic Glycan Labeling in Arabidopsis thaliana

Zhu

Chen

2017

ChemBioChem

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Metabolic glycan labeling (MGL) has gained wide utility and has become a useful tool for probing glycosylation in living systems. For the past three decades, the development and application of MGL have mostly focused on animal glycosylation. Recently, exploiting MGL for studying plant glycosylation has gained interest. Here, we describe a systematic evaluation of MGL for fluorescence imaging of root glycans in Arabidopsis thaliana. Nineteen monosaccharide analogues containing a bioorthogonal group (azide, alkyne, or cyclopropene) were synthesized and evaluated for metabolic incorporation into root glycans. Among these unnatural sugars, 14 (including three new compounds) were evaluated in plants for the first time. Our results showed that five unnatural sugars metabolically labeled root glycans efficiently, and enabled fluorescence imaging by bioorthogonal conjugation with fluorophores. We optimized the experimental procedures for MGL in Arabidopsis. Finally, distinct distribution patterns of the newly synthesized glycans were observed along the root developmental zones, thus indicating regulated biosynthesis of glycans during root development. We envision that MGL will find broad applications in plant glycobiology.

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Identification of anN‐acetylglucosamine kinase essential for UDP‐N‐acetylglucosamine salvage synthesis inArabidopsis

Cited by 21 publications

References 20 publications

Seasonal Alterations in Organic Phosphorus Metabolism Drive the Phosphorus Economy of Annual Growth in F. sylvatica Trees on P-Impoverished Soil

Seasonal Alterations in Organic Phosphorus Metabolism Drive the Phosphorus Economy of Annual Growth in F. sylvatica Trees on P-Impoverished Soil

Metabolic Labeling and Imaging of N‐Linked Glycans in Arabidopsis Thaliana

Expanding the Scope of Metabolic Glycan Labeling in Arabidopsis thaliana

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