Identification of an<i>Arabidopsis</i>mitochondrial succinate–fumarate translocator

Catoni, Elisabetta; Schwab, Rebecca; Hilpert, Melanie; Desimone, Marcelo; Schwacke, Rainer; Flügge, Ulf Ingo; Schumacher, Karin; Frommer, Wolf B.

doi:10.1016/s0014-5793(02)03782-1

Cited by 58 publications

(71 citation statements)

References 31 publications

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“…5) showed a progressive significant increase in the transcript level of isocitrate lyase (the enzyme responsible for the conversion of isocitrate to succinate and glyoxylate during glyoxysomal fatty acid degradation by b-oxidation) during the period of reserve accumulation. The recent identification of a mitochondrial succinate fumarate transporter (Catoni et al, 2003) and evidence that glyoxysomal activity associated with oil degradation is up-regulated during the late stages of seed maturation (Chia et al, 2005), are in keeping with the results obtained from this study.…”

Section: Functions Of the Mitochondria And Glyoxysome During Seed Matsupporting

confidence: 91%

Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches

Fait

Angelovici²,

Less³

et al. 2006

Plant Physiology

389

418

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While the metabolic networks in developing seeds during the period of reserve accumulation have been extensively characterized, much less is known about those present during seed desiccation and subsequent germination. Here we utilized metabolite profiling, in conjunction with selective mRNA and physiological profiling to characterize Arabidopsis (Arabidopsis thaliana) seeds throughout development and germination. Seed maturation was associated with a significant reduction of most sugars, organic acids, and amino acids, suggesting their efficient incorporation into storage reserves. The transition from reserve accumulation to seed desiccation was associated with a major metabolic switch, resulting in the accumulation of distinct sugars, organic acids, nitrogen-rich amino acids, and shikimate-derived metabolites. In contrast, seed vernalization was associated with a decrease in the content of several of the metabolic intermediates accumulated during seed desiccation, implying that these intermediates might support the metabolic reorganization needed for seed germination. Concomitantly, the levels of other metabolites significantly increased during vernalization and were boosted further during germination sensu stricto, implying their importance for germination and seedling establishment. The metabolic switches during seed maturation and germination were also associated with distinct patterns of expression of genes encoding metabolism-associated gene products, as determined by semiquantitative reverse transcription-polymerase chain reaction and analysis of publicly available microarray data. When taken together our results provide a comprehensive picture of the coordinated changes in primary metabolism that underlie seed development and germination in Arabidopsis. They furthermore imply that the metabolic preparation for germination and efficient seedling establishment initiates already during seed desiccation and continues by additional distinct metabolic switches during vernalization and early germination.

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Section: Functions Of the Mitochondria And Glyoxysome During Seed Matsupporting

confidence: 91%

Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches

Fait

Angelovici²,

Less³

et al. 2006

Plant Physiology

389

418

View full text Add to dashboard Cite

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“…2C) as are also most of the fatty acid degradation enzymes 17 reflecting the importance of a continuous supply of energy for relocalization of nutrients to reproductive parts. The PP2A-B'z holoenzyme may possibly be involved in the regulation of the mitochondrial succinate/fumarate translocator, 18 or affect the enzymes that are involved in the succinate conversion into malate. This needs further investigation.…”

Section: Evidence For B'z Involvement In Regulation Of Energy Metabolismmentioning

confidence: 99%

Protein phosphatase 2A regulatory subunits affecting plant innate immunity, energy metabolism, and flowering time – joint functions among B'η subfamily members

Kataya

Heidari

Lillo

2015

Plant Signaling & Behavior

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“…In recent years several of these transporters have been cloned and investigated at the molecular and biochemical level (22,(31)(32)(33)(34). These studies revealed that some members of the MCF in plants can be present in the plastid membranes (35)(36)(37).…”

mentioning

confidence: 99%

Molecular Identification and Functional Characterization of Arabidopsis thaliana Mitochondrial and Chloroplastic NAD+ Carrier Proteins

Palmieri

Rieder

Ventrella

et al. 2009

Journal of Biological Chemistry

154

172

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The Arabidopsis thaliana L. genome contains 58 membrane proteins belonging to the mitochondrial carrier family. Two mitochondrial carrier family members, here named AtNDT1 and AtNDT2, exhibit high structural similarities to the mitochondrial nicotinamide adenine dinucleotide (NAD ؉ ) carrierScNDT1 from bakers' yeast. Expression of AtNDT1 or AtNDT2 restores mitochondrial NAD ؉ transport activity in a yeast mutant lacking ScNDT. Localization studies with green fluorescent protein fusion proteins provided evidence that AtNDT1 resides in chloroplasts, whereas only AtNDT2 locates to mitochondria. Heterologous expression in Escherichia coli followed by purification, reconstitution in proteoliposomes, and uptake experiments revealed that both carriers exhibit a submillimolar affinity for NAD ؉ and transport this compound in a counterexchange mode. Among various substrates ADP and AMP are the most efficient counter-exchange substrates for NAD ؉ .Atndt1-and Atndt2-promoter-GUS plants demonstrate that both genes are strongly expressed in developing tissues and in particular in highly metabolically active cells. The presence of both carriers is discussed with respect to the subcellular localization of de novo NAD ؉ biosynthesis in plants and with respect to both the NAD ؉ -dependent metabolic pathways and the redox balance of chloroplasts and mitochondria.Nucleotides are metabolites of enormous importance for all living cells. They are the essential building blocks for DNA and RNA synthesis, energize most anabolic and many catabolic reactions, and fulfill critical functions in intracellular signal transduction (1, 2). Moreover, nucleotides serve as cofactors for a wide number of enzymes and are, with water, the most highly connected compounds within the metabolic network (3). Among these co-factors nicotinamide adenine dinucleotides are widely used for reductive/oxidative processes, playing important roles in the operation and control of a wide range of dehydrogenase activities. Accordingly, nucleotides are essential in nearly all cell organelles, and transport of these solutes into mitochondria, plastids, the endoplasmic reticulum, the Golgi apparatus, and peroxisomes has been observed (4 -7).Two types of nucleotide transport proteins have been identified to date at the molecular level: nucleotide transporter (NTT) 2 type carriers and members of the mitochondrial carrier family. The former transporters occur in plastids from all plants (8) and in a limited number of intracellular pathogenic bacteria (9). Most NTT-type carrier proteins catalyze an ATP/ADPϩP i counter-exchange mode of transport (10 -13), but several bacterial NTT proteins mediate either H ϩ /nucleotide transport or NAD ϩ /ADP counter-exchange (12,14,15). With the exception of the bacterial NAD ϩ /ADP carrier (14), all NTT proteins exhibit 12 predicted trans-membrane domains, whereas none of the NTT proteins share structural or domain similarities to members of the mitochondrial carrier family (11).Carriers belonging to the mitochondrial carrier family (MC...

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Identification of anArabidopsismitochondrial succinate–fumarate translocator

Cited by 58 publications

References 31 publications

Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches

Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches

Protein phosphatase 2A regulatory subunits affecting plant innate immunity, energy metabolism, and flowering time – joint functions among B'η subfamily members

Molecular Identification and Functional Characterization of Arabidopsis thaliana Mitochondrial and Chloroplastic NAD+ Carrier Proteins

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