Identification of an Entomopathogenic Bacterium, Serratia sp. ANU101, and Its Hemolytic Activity

Kim, Yonggyun; Kim, Keunseob; Seo, Ji-Ae; Shrestha, Sony; Kim, Hosanna H.; Nalini, Madanagopal; Yi, Yanan

doi:10.4014/jmb.0806.360

Cited by 9 publications

(9 citation statements)

References 29 publications

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“…For example, specialization with particular bacteria is a hallmark EPN characteristic, and monospecificity between one nematode and one genus of bacteria or even one symbiont species is commonly observed among these taxa [7]. However, growing evidence of promiscuous relationships between EPNs and their bacterial symbionts suggests that this may not be as common as originally thought (e.g., [28]–[30]). Although most Heterorhabditis and Steinernema symbionts localize to the nematode intestine, there are excellent examples of nematode–bacteria symbioses in other body sites (e.g., [31]).…”

Section: Specialization Of Epnsmentioning

confidence: 99%

An Entomopathogenic Nematode by Any Other Name

et al. 2012

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Section: Specialization Of Epnsmentioning

confidence: 99%

An Entomopathogenic Nematode by Any Other Name

et al. 2012

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“…The aqueous phases containing nucleic acids were transferred to new 1.5 mL microtubes and stored at À20°C. Primers used to amplify the 16S rDNA region were: 5 0 -gAA gAg TTT gAT CAT ggC TC-3 0 (sense) and 5 0 -Aag gAg gTg ATC Cag CCg CA-3 0 (antisense) (Kim et al, 2009). Primers used for sequencing the amplified fragments were: 5 0 -GAA GAG TTT GAT CAT GGC TC-3 0 ; 5 0 -AAG GAG GTG ATC CAG CCG CA-3 0 ; 5 0 -CTC CTA CGG GAG GCA GCA GTG GGG-3 0 (16S362f); 5 0 -CGA AAG CGT GGG GAG CAA ACA GG-3 0 (16S786f); 5-TGC GCT CGT TGC GGG ACT TAA CC-3 0 (16S760r) and 5 0 -GAC TAC CAG GGT ATC TAA TCC TG-3 0 (16S455r) (Cruz et al, 2001;Kim et al, 2009).…”

Section: Extraction Of Bacterial Dna and Sequencing Of The 16s Genementioning

confidence: 99%

Characterization of entomopathogenic nematodes and symbiotic bacteria active against Spodoptera frugiperda (Lepidoptera: Noctuidae) and contribution of bacterial urease to the insecticidal effect

et al. 2012

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In this study we screened soil nematodes collected in the south region of Brazil for pathogenicity against S. frugiperda. Symbiotic bacteria associated with these nematodes were isolated and characterized. We also evaluated urease production by the symbiotic bacteria in vitro and along the course of infection in S. frugiperda and demonstrated that urease production correlated positively to their entomopathogenicity.

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“…Bt tolerance is associated with loss or modification of Bt receptors (Ferré and Van Rie 2002;Gahan et al 2001), altered proteolysis of Bt toxin(s) (Oppert et al 1997), and recovery of damaged midgut epithelial cells (Forcada et al 1999). In previous study, Kim et al (2009) reported that the Serratia sp. isolate significantly elevated Bt pathogenicity against insect pest Spodoptera exigua.…”

Section: Introductionmentioning

confidence: 99%

“…recently reported the excellent potential of B. sphaericus for larvae of malarial vector, Anopheles stephensi (0.051%, 0.057%, 0.062%, and 0.066% for I-IV instar, respectively), and B. thuringiensis israelensis against the first to fourth instar larvae of Culex quinquefasciatus of values LC 50 = 9.332%, 9.832%, 10.212%, and 10.622%, and LC 90 =15.225%, 15.508%, 15.887%, and 15.986% larvae.The various reports on insecticidal potential of Serratia species against agriculture pest(Grimont and Grimont 1978;Nunez-Valdez et al 2008;Kim et al. 2009) prompted us to investigate insecticidal potential of Serratia species against mosquito species.…”

mentioning

confidence: 99%

Insecticidal potency of bacterial species Bacillus thuringiensis SV2 and Serratia nematodiphila SV6 against larvae of mosquito species Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus

2011

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The tremendous worldwide efforts to isolate novel mosquito larvicidal bacteria with improved efficacy present significant promise to control vector-borne diseases of public health importance. In the present study, two native bacterial isolates, Bacillus thuringiensis (Bt SV2) and Serratia species (SV6) were evaluated for mosquito larvicidal potential against the early fourth instar larvae of Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus with reference to B. thuringiensis subsp. israelensis (Bti) H 14. The native Gram-positive, spore-forming Bt SV2 isolate showed 100% mortality against early fourth instars of Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus, in parallel to Bti H14 strain. After 24 h, Bt SV2 showed 98%, 89%, and 80.67%, and Bti H14 showed 92%, 98.33%, and 60% mortality against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus, respectively. Serratia SV6 showed highest activity against Culex quinquefasciatus (100%) followed by Anopheles stephensi (95%) and Aedes aegypti (91%) after 48 h of exposure. The Gram-negative Serratia SV6 showed delayed toxicity compared to Bti H14 and Bt SV2 against early fourth instars of Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus. The relative mortality of all treatments after 12-h exposures showed the varied toxicity with respect to exposure time, bacterial treatment, and mosquito species. Genetic relatedness of the strains was confirmed on the basis of phylogenetic reconstructions based on alignment of 16S rRNA gene sequences which indicated a strong clustering of the strain SV2 with B. thuringiensis and the strain SV6 with Serratia nematodiphila. In conclusion, the native isolate B. thuringiensis SV2 showed significant toxicity while Serratia SV6 showed less and delayed toxicity against several mosquito species compared with BtiH14. They may be used as novel bacterial insecticidal agents in mosquito vector-borne disease control. To our knowledge, this is the first report on mosquito larvicidal potential of Serratia species.

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Identification of an Entomopathogenic Bacterium, Serratia sp. ANU101, and Its Hemolytic Activity

Cited by 9 publications

References 29 publications

An Entomopathogenic Nematode by Any Other Name

An Entomopathogenic Nematode by Any Other Name

Characterization of entomopathogenic nematodes and symbiotic bacteria active against Spodoptera frugiperda (Lepidoptera: Noctuidae) and contribution of bacterial urease to the insecticidal effect

Insecticidal potency of bacterial species Bacillus thuringiensis SV2 and Serratia nematodiphila SV6 against larvae of mosquito species Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus

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