Identification of an Elicitin Gene Cluster in<i>Phytophthora Cinnamomi</i>

Duclos, Joana; Fauconnier, A; Coelho, A. C.; Bollen, Alex; Cravador, A.; Godfroid, Edmond

doi:10.3109/10425179809105210

Cited by 25 publications

(31 citation statements)

References 21 publications

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“…In P. cinnamomi a gene cluster consisting of four elicitins genes was identified (Duclos et al, 1998) and the crystal structure of cinnamomin was determined (Rodrigues et al, 2006). The elicitin α-cinnamomin was shown to restrict the invasion of P. cinnamomi in root cortical tissues, preventing vascular colonization of cork and holm oak (Medeira et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Alpha Cinnamomin Elicits a Defence Response Against Phytophthora Cinnamomi in Castanea Sativa

Medeira¹,

Maia²,

Ribeiro³

et al. 2012

Acta Hortic.

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Phytophthora cinnamomi and P. cambivora are considered as the causal agents of Castanea sativa ink disease. These soil-borne plant pathogens invade and destroy the root system leading to the death of the trees.Most Phytophthora species secrete elicitins, a group of unique highly conserved proteins that are able to enhance plant defence responses in a systemic acquired resistance manner against infection by several pathogens. A cluster of four elicitin genes was identified in P. cinnamomi. In previous works one of these elicitins, α-cinnamomin was shown to restrict the invasion of root cortical tissues by P. cinnamomi preventing vascular colonization in cork and holm oak. In the present work, roots of chestnut plantlets grown in vitro were allowed to absorb α-cinnamomin at 100 µg/ml for two days before being inoculated with P. cinnamomi. The effects of this elicitin on host-pathogen interaction were studied at histological and ultrastructural levels.P. cinnamomi was restricted to the outer cortex of 65% of the roots pre-treated with α-cinnamomin. In these roots, the vascular cylinders were free of pathogen. On the contrary, the pathogen reached the vascular cylinder, penetrating the phloem and xylem vessels in all non-treated assayed roots. The signs of pathogen degradation in the cortical parenchyma, mainly in the intercellular spaces, and the increase of a physical barrier in epidermal and sub-epidermal cell wall-media lamella and intercellular spaces by impregnation with phenol-like compounds strongly suggest that α-cinnamomin induced in chestnut defence reactions against P. cinnamomi.

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Section: Introductionmentioning

confidence: 99%

Alpha Cinnamomin Elicits a Defence Response Against Phytophthora Cinnamomi in Castanea Sativa

Medeira¹,

Maia²,

Ribeiro³

et al. 2012

Acta Hortic.

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“…Studies in two other Phytophthora species also demonstrated clustering of elicitin genes. In both, P. cinnamomi and P. cryptogea, a 6-kb genomic region encompasses four elicitin genes (Panabieres et al 1995;Duclos et al 1998). In P. infestans the inf1 gene is highly expressed in mycelium but not in sporangia or cysts (Kamoun et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Elicitin genes in Phytophthora infestans are clustered and interspersed with various transposon-like elements

et al. 2005

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Sequencing and annotation of a contiguous stretch of genomic DNA (112.3 kb) from the oomycete plant pathogen Phytophthora infestans revealed the order, spacing and genomic context of four members of the elicitin (inf) gene family. Analysis of the GC content at the third codon position (GC3) of six genes encoded in the region, and a set of randomly selected coding regions as well as random genomic regions, showed that a high GC3 value is a general feature of Phytophthora genes that can be exploited to optimize gene prediction programs for Phytophthora species. At least one-third of the annotated 112.3-kb P. infestans sequence consisted of transposons or transposon-like elements. The most prominent were four Tc3/gypsy and Tc1/copia type retrotransposons and three DNA transposons that belong to the Tc1/mariner, Pogo and PiggyBac groups, respectively. Comparative analysis of other available genomic sequences suggests that transposable elements are highly heterogeneous and ubiquitous in the P. infestans genome.

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“…The deduced peptide comprised 119 aa, including the peptide signal sequence, and displayed a predicted pI of 3.95. From alignment with known elicitin sequences, it was considered to belong to class II of highly acidic elicitins and was accordingly called HAE1 (11,26).…”

Section: Resultsmentioning

confidence: 99%

“…Although elicitin genes are not appropriate for phylogenetic studies because of their multigenic family feature, they can be used as a tool for identification purposes since the amino acid sequence of a given elicitin may provide a signature at the species level (25). In addition, the 3Ј untranslated regions (3ЈUTRs) of class I elicitin genes are strictly conserved within an individual species but diverge between species to such an extent that sequence alignment is almost impossible (1,6,11;Panabières et al,unpublished results).…”

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confidence: 99%

Distribution and Expression of Elicitin Genes in the Interspecific Hybrid OomycetePhytophthora alni

Ioos

Panabières²,

Industri³

et al. 2007

Appl Environ Microbiol

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Phytophthora alni subsp. alni, P. alni subsp. multiformis, and P. alni subsp. uniformis are responsible for alder disease in Europe. Class I and II elicitin gene patterns of P. alni subsp. alni, P. alni subsp. multiformis, P. alni subsp. uniformis, and the phylogenetically close species P. cambivora and P. fragariae were studied through mRNA sequencing and 3 untranslated region (3UTR)-specific PCRs and sequencing. The occurrence of multiple 3UTR sequences in association with identical elicitin-encoding sequences in P. alni subsp. alni indicated duplication/recombination events. The mRNA pattern displayed by P. alni subsp. alni demonstrated that elicitin genes from all the parental genomes are actually expressed in this allopolyploid taxon. The complementary elicitin patterns resolved confirmed the possible involvement of P. alni subsp. multiformis and P. alni subsp. uniformis in the genesis of the hybrid species P. alni subsp. alni. The occurrence of multiple and common elicitin gene sequences throughout P. cambivora, P. fragariae, and P. alni sensu lato, not observed in other Phytophthora species, suggests that duplication of these genes occurred before the radiation of these species.

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Identification of an Elicitin Gene Cluster inPhytophthora Cinnamomi

Cited by 25 publications

References 21 publications

Alpha Cinnamomin Elicits a Defence Response Against Phytophthora Cinnamomi in Castanea Sativa

Alpha Cinnamomin Elicits a Defence Response Against Phytophthora Cinnamomi in Castanea Sativa

Elicitin genes in Phytophthora infestans are clustered and interspersed with various transposon-like elements

Distribution and Expression of Elicitin Genes in the Interspecific Hybrid OomycetePhytophthora alni

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