Identification of amygdalin and its major metabolites in rat urine by LC–MS/MS

Ge, B.Y.; Chen, H.X.; Han, Fengmei; Chen, Y.

doi:10.1016/j.jchromb.2007.07.036

Cited by 34 publications

(21 citation statements)

References 21 publications

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“…1 Amygdaline, the substance initially found in plants of the rosaceous family, has been widely used to treat asthma, aplastic anemia and tumors in oriental medicine. 2 Furthermore, it has been proved that glycosides readily release aglycones straightforwardly in the processing of plants maturation, gather and industrial processing because of enzymolysis. 3 By enzymatic or acid hydrolysis, the glycosides in many vegetables, fruits and fragrant plants could decomposed into monoterpene alcohols and aromatic alcohols, which were important fruity aroma.…”

Section: Introductionmentioning

confidence: 99%

Identification of Glycoside Compounds from Tobacco by High Performance Liquid Chromatography/Electrospray Ionization Linear Ion-Trap Tandem Mass Spectrometry Coupled with Electrospray Ionization Orbitrap Mass Spectrometry

Jia¹,

Zhu²,

Zhang³

et al. 2016

J. Braz. Chem. Soc.

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In order to comprehensively screen and identify the glycoside compounds in tobacco, a simple, rapid and sensitive method of high performance liquid chromatography/electrospray ionization linear ion-trap tandem mass spectrometry (HPLC-ESI-LIT/MS n ) coupled with electrospray ionization orbitrap mass spectrometry (ESI-Orbitrap-MS) was developed for the first time. As a result, twenty-two glycoside compounds, including eleven alcoholic glycosides, eight phenolic glycosides, two ester glycosides and an indole glycoside, were reliably identified from tobacco with high mass accuracy (within 5 mDa). Among them, four compounds were confirmed as novel molecules and other four compounds, as far as we know, were not reported previously in tobacco. This study provided a useful tool to identify the new structures of glycoside compounds in natural products, especially when there were no reference compounds available.

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Section: Introductionmentioning

confidence: 99%

Identification of Glycoside Compounds from Tobacco by High Performance Liquid Chromatography/Electrospray Ionization Linear Ion-Trap Tandem Mass Spectrometry Coupled with Electrospray Ionization Orbitrap Mass Spectrometry

Jia¹,

Zhu²,

Zhang³

et al. 2016

J. Braz. Chem. Soc.

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“…[12][13][14] However, few studies have been reported on the pharmacokinetics of pure amygdalin compared with amygdalin contained in decoctions of peach seeds. Herbal medicines are mixtures containing many ingredients, and herb-drug interactions or herb-herb interactions are common.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Pharmacokinetics in Rats: Differences between Pure Amygdalin and Amygdalin in a Decoction of Peach Seeds

Chen¹,

Yan²,

Kim³

et al. 2012

Bulletin of the Korean Chemical Society

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The pharmacokinetics of prunasin after oral administration of amygdalin or a decoction of peach seeds was determined and compared in rats. A C 18 column was used for separation at a column temperature of 25 o C. The mobile phase consisted of 20% aqueous acetonitrile, and the flow rate was 0.5 mL/min. After oral administration of a decoction of peach seeds, prunasin was absorbed rapidly, reaching a maximum plasma concentration (C max ) of 62.1 mg/L within 45 min. After oral administration of amygdalin, the absorption of prunasin was delayed. The C max of prunasin was 42.9 mg/L and was reached at 60 min. Values for the pharmacokinetic parameters of prunasin, including T max , C max , AUC, T 1/2 , CL/F, and V1/F, were significantly different for the oral administration of amygdalin compared with that of a decoction of peach seeds.

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“…HPLC-MS n is a powerful modern tool for the identification of substances in biofluids (Ge et al, 2007;Han et al, 2007;Lam and Ramanathan, 2002;Rudewicz and Straub, 1986;Wang et al, 2005;Yang et al, 2006;Yu et al, 1999;Zhang et al, 2003Zhang et al, , 2004. This approach has the advantages of sensitivity and specificity, and is considered less time-consuming and labor-intensive than other methods, such as HPLC and GC-MS.…”

Section: Introductionmentioning

confidence: 99%

Identification of major alkaloids and steroidal saponins in rat serum by HPLC‐diode array detection‐MS/MS following oral administration of Huangbai‐Zhimu herb‐pair Extract

Fan

Tang

et al. 2008

Biomedical Chromatography

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Huangbai-Zhimu herb-pair (HBZMHP) is a widely used Chinese traditional medicine formula in treating various diseases; however, its active components have remained unknown. In this paper, serum chemistry and combined high-performance liquid chromatography (HPLC), diode-array detection and mass-spectrometry (MS) techniques were used to study the constituents of HBZMHP extract absorbed into rat serum after oral administration. A total of nine characteristic HPLC peaks in the TIC chromatograms were identified as magnoflorine (1), menisperine (2), palmatine (3), berberine (4), timosaponin N or timosaponin E1 (5), timosaponin D (6), timosaponin BIII, anemarsaponin C or xilingsaponin B (7) timosaponin BII (8) and timosaponin AIII (9). All of the identified peaks were constituents of HBZMHP extract. The results narrow the range of active compounds to be found in HBZMHP extract, and pave the way for the follow-up action mechanism research.

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Identification of amygdalin and its major metabolites in rat urine by LC–MS/MS

Cited by 34 publications

References 21 publications

Identification of Glycoside Compounds from Tobacco by High Performance Liquid Chromatography/Electrospray Ionization Linear Ion-Trap Tandem Mass Spectrometry Coupled with Electrospray Ionization Orbitrap Mass Spectrometry

Identification of Glycoside Compounds from Tobacco by High Performance Liquid Chromatography/Electrospray Ionization Linear Ion-Trap Tandem Mass Spectrometry Coupled with Electrospray Ionization Orbitrap Mass Spectrometry

Metabolic Pharmacokinetics in Rats: Differences between Pure Amygdalin and Amygdalin in a Decoction of Peach Seeds

Identification of major alkaloids and steroidal saponins in rat serum by HPLC‐diode array detection‐MS/MS following oral administration of Huangbai‐Zhimu herb‐pair Extract

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