Identification of amphioxus protein disulfide isomerase as both an enzyme and an immunocompotent factor

Ma, Zhiyu; Tan, Yibo; Qu, Baozhen; Gao, Zhan; Zhang, Shicui

doi:10.1016/j.dci.2021.104238

Cited by 1 publication

(1 citation statement)

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“…Interestingly, the expression of genes associated with GH3 and SAUR were up-regulated after 16F16 treatment (Supplementary Table S6). Previous research has shown that auxin-induced GH3 genes can mediate the inactivation of IAA by conjugation, which further attenuates auxin signal transduction [45]. In this study, up-regulated expression of the GH3 gene after 16F16 treatment enhanced regulation of IAA inactivation and further attenuated auxin signal transduction.…”

Section: Discussionsupporting

confidence: 46%

Integrated Transcriptome and Proteome Analysis Reveals the Regulatory Mechanism of Root Growth by Protein Disulfide Isomerase in Arabidopsis

Liu,

Song,

Yan

et al. 2024

IJMS

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Protein disulfide isomerase (PDI, EC 5.3.4.1) is a thiol-disulfide oxidoreductase that plays a crucial role in catalyzing the oxidation and rearrangement of disulfides in substrate proteins. In plants, PDI is primarily involved in regulating seed germination and development, facilitating the oxidative folding of storage proteins in the endosperm, and also contributing to the formation of pollen. However, the role of PDI in root growth has not been previously studied. This research investigated the impact of PDI gene deficiency in plants by using 16F16 [2-(2-Chloroacetyl)-2,3,4,9-tetrahydro-1-methyl-1H-pyrido[3,4-b]indole-1-carboxylic acid methyl ester], a small-molecule inhibitor of PDI, to remove functional redundancy. The results showed that the growth of Arabidopsis roots was significantly inhibited when treated with 16F16. To further investigate the effects of 16F16 treatment, we conducted expression profiling of treated roots using RNA sequencing and a Tandem Mass Tag (TMT)-based quantitative proteomics approach at both the transcriptomic and proteomic levels. Our analysis revealed 994 differentially expressed genes (DEGs) at the transcript level, which were predominantly enriched in pathways associated with “phenylpropane biosynthesis”, “plant hormone signal transduction”, “plant−pathogen interaction” and “starch and sucrose metabolism” pathways. Additionally, we identified 120 differentially expressed proteins (DEPs) at the protein level. These proteins were mainly enriched in pathways such as “phenylpropanoid biosynthesis”, “photosynthesis”, “biosynthesis of various plant secondary metabolites”, and “biosynthesis of secondary metabolites” pathways. The comprehensive transcriptome and proteome analyses revealed a regulatory network for root shortening in Arabidopsis seedlings under 16F16 treatment, mainly involving phenylpropane biosynthesis and plant hormone signal transduction pathways. This study enhances our understanding of the significant role of PDIs in Arabidopsis root growth and provides insights into the regulatory mechanisms of root shortening following 16F16 treatment.

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Section: Discussionsupporting

confidence: 46%