Identification of amino acid changes in the envelope glycoproteins of bovine viral diarrhea viruses isolated from alpaca that may be involved in host adaptation

Neill, John D.; Dubovi, Edward J.; Ridpath, Julia F.

doi:10.1016/j.vetmic.2015.06.007

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…Mutations affecting this region were demonstrated to dramatically increase the secretion of the E rns protein from infected cells (Tews and Meyers, 2007;Burrack et al, 2012). Conserved changes in the E2 and E rns coding regions have also been reported in BVDV-1b isolates obtained from alpacas and were shown to increase the ability of these viruses to infect alpaca cells (Neill et al, 2015). It is possible that the amino acid changes detected in this study may have optimized the ability of these viral isolates to infect and establish persistent infection in swine.…”

Section: Discussionsupporting

confidence: 63%

Changes Introduced in the Open Reading Frame of Bovine Viral Diarrhea Virus During Serial Infection of Pregnant Swine

et al. 2020

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Bovine viral diarrhea virus (BVDV) is one of the most economically important viruses of cattle, but this pathogen is also able to infect pigs, camelids, and a wide range of domestic and wild ruminants. BVDV isolates circulating in animal populations are genetically and antigenically highly diverse. Acute BVDV infections in cattle cause the introduction of many substitutions in the viral genome. Serial infection of pregnant sheep with a BVDV-1b isolate of bovine origin was also associated with great numbers of substitutions. To our knowledge, genomic changes arising during BVDV infections in swine have not been investigated. The purpose of this study was to investigate the changes occurring in the open reading frame (ORF) of BVDV during serial infection of pregnant swine with a BVDV isolate of bovine origin. The BVDV-1b isolate AU526 was serially passaged in six pregnant gilts, two of which gave birth to live piglets congenitally infected with BVDV. The complete ORF sequences of 14 BVDV isolates obtained from pregnant gilts and their piglets were determined. Their analysis revealed that serial transmission of AU526 in pregnant swine resulted in many genomic changes. All isolates of porcine origin shared 32 nucleotide and 12 amino acid differences with the virus inoculum AU526. These changes were detected after a single passage in pregnant swine and were conserved during the subsequent five passages. Amino acid changes occurred primarily in genomic regions encoding the BVDV structural proteins E2 and E rns . These results suggest that BVDV infections in pregnant swine may contribute significantly to the genetic variability of BVDV and lead to the appearance of adaptive changes.

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Section: Discussionsupporting

confidence: 63%

Changes Introduced in the Open Reading Frame of Bovine Viral Diarrhea Virus During Serial Infection of Pregnant Swine

et al. 2020

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“…Host-specific amino acid changes in the E2 coding region were described during serial infection of pregnant cattle and sheep ( Paton et al, 1997 ). Conserved amino acid changes in the E2 and E rns coding regions were also reported in BVDV-1b isolates from alpacas ( Neill et al, 2015 ). Analogously, most conserved changes detected in ovine isolates in this study occurred in the same coding regions of the BVDV genome.…”

Section: Discussionmentioning

confidence: 67%

Identification of Conserved Amino Acid Substitutions During Serial Infection of Pregnant Cattle and Sheep With Bovine Viral Diarrhea Virus

et al. 2018

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Bovine viral diarrhea virus (BVDV) is an economically important pathogen of cattle that can also infect a wide range of domestic and wild species including sheep, goats, deer, camelids, and pigs. BVDV isolates are genetically highly diverse and previous work demonstrated that many substitutions were introduced in the viral genome during acute infections in cattle. In contrast, only limited information exists regarding changes occurring during BVDV infections in species other than cattle. The purpose of this study was to determine the changes introduced in the open reading frame (ORF) of the BVDV genome during serial infection of pregnant cattle and sheep with an isolate of bovine origin. Serial experimental inoculations were performed in six pregnant heifers and six pregnant ewes using BVDV-1b isolate AU526 in the first heifer and ewe, and serum from the preceding acutely infected dam thereafter. Complete ORF sequences were determined for 23 BVDV-1b isolates including AU526, one isolate from each pregnant dam, and one isolate from each BVDV-positive offspring born to these dams. Sequence comparison revealed that greater numbers of substitutions occurred during serial infection of pregnant sheep than of pregnant cattle. Furthermore, multiple host-specific amino acid changes were gradually introduced and conserved. These changes were more abundant in ovine isolates and occurred primarily in the E2 coding region. These results suggest that BVDV infections in heterologous species may serve as a significant source of viral genetic diversity and may be associated with adaptive changes.

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“…We decided to introduce this event to prove that the results of the tests must be treated with consideration; comparison of the results of the different methods and visual re-evaluation of the graphs are always necessary, as it was suggested previously (Leal et al, 2012). The major parent (Neill et al, 2015) was demonstrated in the USA, the closest minor parent in the UK. Since close relatives of the real parents were not sequenced in this case, the P value is relatively low.…”

Section: Discussionmentioning

confidence: 99%

Demonstration of homologous recombination events in the evolution of bovine viral diarrhoea virus by in silico investigations

Kővágó

Hornyák

Kékesi

et al. 2016

Acta Veterinaria Hungarica

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Complete genome sequences of bovine viral diarrhoea virus types 1 and 2 (BVDV-1 and 2) deposited in the GenBank were submitted to bioinformatic analysis using a recombination-detecting software. The results indicate that recombination events are not rare in the case of BVDV, which frequently causes immunotolerance and, consequently, persistent infection in calves. The lack of specific immunity provides an ideal possibility for multiple infections by antigenically related but genetically different BVDV strains, and hence recombinations may occur. Among the 62 BVDV-1 genomes five recombinants and their possible parent strains, while among the 50 BVDV-2 genomes one simple recombinant and its parent strains were identified, which were supported by extremely strong probability values (P values varying between 1.26 × 10 -4 and 1.58 × 10 -310). Besides the newly identified recombinants, recombination events described previously were confirmed, but in some of these cases former information was completed with new data, or different parent(s) were suggested by the programme (RDP 4.46 BETA) used in this study.

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Identification of amino acid changes in the envelope glycoproteins of bovine viral diarrhea viruses isolated from alpaca that may be involved in host adaptation

Cited by 6 publications

References 24 publications

Changes Introduced in the Open Reading Frame of Bovine Viral Diarrhea Virus During Serial Infection of Pregnant Swine

Changes Introduced in the Open Reading Frame of Bovine Viral Diarrhea Virus During Serial Infection of Pregnant Swine

Identification of Conserved Amino Acid Substitutions During Serial Infection of Pregnant Cattle and Sheep With Bovine Viral Diarrhea Virus

Demonstration of homologous recombination events in the evolution of bovine viral diarrhoea virus by in silico investigations

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