Identification of Alternative Splicing Events Regulated by an Arabidopsis Serine/Arginine-Like Protein, atSR45a, in Response to High-Light Stress using a Tiling Array

Yoshimura, Kazuya; Mori, Tatsuya; Yokoyama, Kunihiro; Koike, Yoshiyuki; Tanabe, Noriaki; Sato, Nobuo; Takahashi, Hiro; Maruta, Takanori; Shigeoka, Shigeru

doi:10.1093/pcp/pcr115

Cited by 30 publications

(31 citation statements)

References 61 publications

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“…Indeed, the expression of SR45 has been reported to show significant changes under sucrose and temperature stress [58], but not in response to ABA or other phytohormones [40,58]. Expression of SR45a is markedly induced by high light stress [59], while DNA microarray studies [60,61,62,63] and RNA-seq analysis of abiotic stress samples [64] have shown that SR45a mRNA abundance is also altered upon heat and water deprivation stresses. Nevertheless, SR45a expression in response to ABA was not investigated until this study.…”

Section: Resultsmentioning

confidence: 99%

Abscisic Acid (ABA) Regulation of Arabidopsis SR Protein Gene Expression

Cruz

Carvalho

Richardson

et al. 2014

IJMS

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Serine/arginine-rich (SR) proteins are major modulators of alternative splicing, a key generator of proteomic diversity and flexible means of regulating gene expression likely to be crucial in plant environmental responses. Indeed, mounting evidence implicates splicing factors in signal transduction of the abscisic acid (ABA) phytohormone, which plays pivotal roles in the response to various abiotic stresses. Using real-time RT-qPCR, we analyzed total steady-state transcript levels of the 18 SR and two SR-like genes from Arabidopsis thaliana in seedlings treated with ABA and in genetic backgrounds with altered expression of the ABA-biosynthesis ABA2 and the ABA-signaling ABI1 and ABI4 genes. We also searched for ABA-responsive cis elements in the upstream regions of the 20 genes. We found that members of the plant-specific SC35-Like (SCL) Arabidopsis SR protein subfamily are distinctively responsive to exogenous ABA, while the expression of seven SR and SR-related genes is affected by alterations in key components of the ABA pathway. Finally, despite pervasiveness of established ABA-responsive promoter elements in Arabidopsis SR and SR-like genes, their expression is likely governed by additional, yet unidentified cis-acting elements. Overall, this study pinpoints SR34, SR34b, SCL30a, SCL28, SCL33, RS40, SR45 and SR45a as promising candidates for involvement in ABA-mediated stress responses.

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Section: Resultsmentioning

confidence: 99%

Abscisic Acid (ABA) Regulation of Arabidopsis SR Protein Gene Expression

Cruz

Carvalho

Richardson

et al. 2014

IJMS

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“…RT-PCR showed that the expression of the selected gene in each mutant was completely abrogated (Figure S3). Some of these genes are known to participate in abiotic stress, such as EPR1 in light rhythm [37], NTL4 in ROS regulation during drought-induced senescence [87] and SR45a in alternative splicing under high light [88].…”

Section: Resultsmentioning

confidence: 99%

Identification of Heat Responsive Genes in Brassica napus Siliques at the Seed-Filling Stage through Transcriptional Profiling

Fan

Yang

et al. 2014

PLoS ONE

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High temperature stress results in yield loss and alterations to seed composition during seed filling in oilseed rape (Brassica napus). However, the mechanism underlying this heat response is poorly understood. In this study, global transcription profiles of 20 d-old siliques of B. napus were analyzed after heat stress using a Brassica 95k EST microarray. The up-regulated genes included many HSF/HSP transcripts and other heat-related marker genes, such as ROF2, DREB2a, MBF1c and Hsa32, reflecting the conservation of key heat resistance factors among plants. Other up-regulated genes were preferentially expressed in heat-stressed silique walls or seeds, including some transcription factors and potential developmental regulators. In contrast, down-regulated genes differed between the silique wall and seeds and were largely tied to the biological functions of each tissue, such as glucosinolate metabolism in the silique wall and flavonoid synthesis in seeds. Additionally, a large proportion (one-third) of these differentially expressed genes had unknown functions. Based on these gene expression profiles, Arabidopsis mutants for eight heat-induced Brassica homologous genes were treated with different heat stress methods, and thermotolerance varied with each mutation, heat stress regimen and plant development stage. At least two of the eight mutants exhibited sensitivity to the heat treatments, suggesting the importance of the respective genes in responding to heat stress. In summary, this study elucidated the molecular bases of the heat responses in siliques during later reproductive stages and provides valuable information and gene resources for the genetic improvement of heat tolerance in oilseed rape breeding.

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“…Expression of P5CDH was found to be regulated by gene silencing via the overlapping gene Sro5 on the complementary DNA strand, but so far, our analyses did not clearly demonstrate an altered regulation of P5CDH in the rsr1-1 mutant (Funck, 2001; Borsani et al, 2005). A recent study on transcriptome changes in a splicing factor mutant suggests that RSR1 / ESP1 itself may be regulated by alternative mRNA processing (Yoshimura et al, 2011). …”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis CstF64-Like RSR1/ESP1 Protein Participates in Glucose Signaling and Flowering Time Control

Funck¹,

Clauß²,

Frommer³

et al. 2012

Front. Plant Sci.

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Mechanisms for sensing and regulating metabolic processes at the cellular level are critical for the general physiology and development of living organisms. In higher plants, sugar signaling is crucial for adequate regulation of carbon and energy metabolism and affects virtually every aspect of development. Although many genes are regulated by sugar levels, little is known on how sugar levels are measured by plants. Several components of the sugar signaling network have been unraveled and demonstrated to have extensive overlap with hormone signaling networks. Here we describe the reduced sugar response1-1 (rsr1-1) mutant as a new early flowering mutant that displays decreased sensitivity to abscisic acid. Both hexokinase1 (HXK1)-dependent and glucose phosphorylation-independent signaling is reduced in rsr1-1. Map-based identification of the affected locus demonstrated that rsr1-1 carries a premature stop codon in the gene for a CstF64-like putative RNA processing factor, ESP1, which is involved in mRNA 3′-end formation. The identification of RSR1/ESP1 as a nuclear protein with a potential threonine phosphorylation site may explain the impact of protein phosphorylation cascades on sugar-dependent signal transduction. Additionally, RSR1/ESP1 may be a crucial factor in linking sugar signaling to the control of flowering time.

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Identification of Alternative Splicing Events Regulated by an Arabidopsis Serine/Arginine-Like Protein, atSR45a, in Response to High-Light Stress using a Tiling Array

Cited by 30 publications

References 61 publications

Abscisic Acid (ABA) Regulation of Arabidopsis SR Protein Gene Expression

Abscisic Acid (ABA) Regulation of Arabidopsis SR Protein Gene Expression

Identification of Heat Responsive Genes in Brassica napus Siliques at the Seed-Filling Stage through Transcriptional Profiling

The Arabidopsis CstF64-Like RSR1/ESP1 Protein Participates in Glucose Signaling and Flowering Time Control

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