Identification of altered MicroRNA expression patterns in synovial sarcoma

Hisaoka, Masanori; Matsuyama, Atsuji; Nagao, Yuhei; Luan, Lan; Kuroda, Toshihiko; Akiyama, Hideo; Kondo, Satoshi; Hashimoto, Hiroshi

doi:10.1002/gcc.20837

Cited by 72 publications

(61 citation statements)

References 29 publications

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“…The expression of miR-125a-3p is reportedly down-regulated in non-small cell lung cancer (19). In contrast, it is up-regulated in synovial sarcoma (20), and overexpression under hypoxic conditions in retinoblastoma has also been observed (21). miR-125a-5p, the partner of miR-125a-3p, is down-regulated in breast cancer (22)(23)(24), ovarian cancer (25), lung cancer (26), and medulloblastoma (27), indicating its tumor suppressive effect in human cancers.…”

Section: Discussionmentioning

confidence: 99%

Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance

et al. 2012

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Abstract. Recent reports have demonstrated that another strand of mature microRNA (miRNA), called microRNA * or 3p (5p) strand, which is generated from the same precursor miRNA (Pre-miR), has a crucial role in cellular function. We previously reported the tumor suppressive effect of miR-125a-5p in gastric cancer. The current study was designed to examine the function and clinical significance of miR-125a-3p, a partner strand of miR-125a-5p, in human gastric cancer. Quantitative RT-PCR was used to evaluate miR-125a-3p expression in 70 gastric cancer cases to determine the clinicopathologic significance of miR-125a-3p expression. In addition, the effect of miR-125a-3p on the proliferation of gastric cancer cells was investigated. Low expression levels of miR-125a-3p were associated with indicators of enhanced malignant potential such as tumor size (p=0.0002), tumor invasion (p=0.0149), lymph node metastasis (p=0.018), liver metastasis (p=0.016), peritoneal dissemination (p=0.03), advanced clinical stage (p=0.0037) and poor prognosis (p=0.0083). Multivariate analysis indicated that low miR-125a-3p expression was an independent prognostic factor for survival, while in vitro assays demonstrated that miR-125a-3p suppressed the proliferation of gastric cancer cells. MiR-125a-3p is a potent prognostic marker in gastric cancer. The clinical significance and tumor suppressive effect of miR-125a-3p, as well as previously reported miR-125a-5p, suggest that the functional role of another strand of the mature form miRNA cannot be ignored, at least in miR-125a biogenesis.

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Section: Discussionmentioning

confidence: 99%

Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance

et al. 2012

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“…Expression profiles of miRNA were analyzed using a 3D-Gene miRNA microarray platform (version 14.0, Toray, Kamakura, Japan) as described previously. 15 More than 900 antisense probes were printed in duplicate spots on the miRNA microchip The annotation and oligonucleotide sequences of the probes conformed to the miRBase miRNA database version 14.0 (http://microrna.sanger.ac.uk/ sequences/). The data were assessed using unsupervised hierarchical clustering.…”

Section: Rna Extraction and Mirna Microarraysmentioning

confidence: 99%

Association of microRNA-21 expression with its targets, PDCD4 and TIMP3, in pancreatic ductal adenocarcinoma

et al. 2012

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Since the discovery of small non-coding RNAs, the analyses of microRNA (miRNA) expression patterns in human cancer have provided new insights into cancer biology. miRNA-21 has been suggested to be one of the miRNAs that have an important role in the development or biological behavior of a variety of malignancies, including pancreatic cancer. This study was conducted to evaluate the relationship between the expression of miRNA-21 and that of its molecular targets, programmed cell death 4 (PDCD4) and tissue inhibitor of metalloproteinase (TIMP3), in pancreatic ductal adenocarcinoma. The study included 65 pancreatic ductal adenocarcinomas and 5 normal pancreatic tissue specimens for comparison. The miRNA expression profiling of five selected pancreatic ductal adenocarcinomas and five normal pancreatic specimens was performed using a microarray platform, and was evaluated by a hierarchical clustering analysis. The miRNA most highly expressed in pancreatic ductal adenocarcinomas (ie, miRNA-21) was further assessed by quantitative real-time reverse transcription PCR (RT-PCR) assays in the 65 pancreatic ductal adenocarcinoma cases. The expression pattern of its molecular targets (eg, PDCD4 and TIMP3) in pancreatic ductal adenocarcinoma was examined immunohistochemically. In the microarray analyses, 28 miRNAs were upregulated in pancreatic ductal adenocarcinoma compared with normal pancreatic tissue, whereas 48 miRNAs were downregulated. miRNA-21 was the most significantly overexpressed miRNA in the pancreatic ductal adenocarcinomas analyzed, and was also highly expressed in 75% of the 65 pancreatic ductal adenocarcinomas examined by real-time RT-PCR. High miRNA-21 expression was correlated with a worse prognosis in the pancreatic ductal adenocarcinoma patients (P ¼ 0.045). The immunohistochemical expression patterns of PDCD4 (reduced nuclear staining pattern) and TIMP3 (downregulated expression) were significantly associated with both the upregulated miR-21 expression (Po0.05) and the poor survival of the patients (Po0.001 and P ¼ 0.001, respectively). Our data suggest that an overexpression of miRNA-21 is, therefore, associated with the biological behavior of pancreatic ductal adenocarcinoma via the downregulation of the expression of tumor suppressors, PDCD4 and TIMP3, thus resulting in tumor progression and the adverse clinical course of pancreatic ductal adenocarcinoma.

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“…The early diagnosis of GC has increased because of the rapid development of several diagnostic techniques, such as chromo endoscopy and random biopsies, carcinoembryonic antigen (CEA) and some conventional biomarkers; however, extremely poor survival rates still remain [5,6]. Although chromo endoscopy and random biopsies are considered to be reliable and credible methods for diagnosis, their applications are limited due to the examination's cost and invasive and unpleasant nature and random biopsy's potential for sampling errors [7].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of circulating microRNAs for gastric cancer in Asian populations: a meta-analysis

et al. 2014

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Gastric cancer (GC) accounts for one of the highest mortality worldwide and particularly in East Asia. Many studies have reported on the potential value of microRNAs (miRNAs) detection for diagnosing GC, but their results have proven inconclusive. The present meta-analysis was conducted to assess the diagnostic value of circulating miRNAs for GC diagnosis. A literature search was carried out in databases (PubMed, Embase, Web of Science, The Cochrane Library, and CNKI) and other sources using combinations of keywords relating to GC, miRNAs, and diagnosis. The values of sensitivity, specificity, positive likelihood ratios (PLR), negative likelihood ratios (NLR), and diagnostic odds ratio (DOR) reported in individual studies were pooled using random-effects models. Potential sources of heterogeneity were assessed with subgroup and meta-regression analyses. The summary receiver operating characteristic (SROC) curve and the area under the curve (AUC) were used to assess the diagnosis accuracy of miRNAs. This meta-analysis included 1,279 patients with GC and 954 healthy controls from 20 publications. The pooled sensitivity, specificity, PLR, NLR, DOR, and AUC were 0.78 (95 % CI: 0.73-0.81), 0.80 (95 % CI: 0.76-0.84), 4.0 (95 % CI: 3.1-6.0), 0.28 (95 % CI: 0.23-0.34), 14 (95 % CI: 10-21), and 0.86 (95 % CI: 0.83-0.89), respectively. Subgroup analyses showed that early stages (I and II) GC were more easily detected than later stages and that multiple miRNAs assays were more accurate than single miRNA assays. Our meta-analysis suggests that miRNAs have a high diagnostic value for GC, especially in its early stages (I and II). In addition, multiple miRNAs assays have a better diagnosis value than single miRNA assays. In conclusion, circulating miRNAs might be used as noninvasive biomarkers for the confirmation of GC detection in Asian populations.

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Identification of altered MicroRNA expression patterns in synovial sarcoma

Cited by 72 publications

References 29 publications

Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance

Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance

Association of microRNA-21 expression with its targets, PDCD4 and TIMP3, in pancreatic ductal adenocarcinoma

Diagnostic value of circulating microRNAs for gastric cancer in Asian populations: a meta-analysis

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