Identification of allergens involved in occupational asthma due to carmine dye

Lizaso, María Teresa; Moneo, Ignacio; Be, García; Acero, S.; Quirce, Santiago; Tabar, A. I.

doi:10.1016/s1081-1206(10)62521-5

Cited by 26 publications

(20 citation statements)

References 10 publications

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“…According to the clinical features and origin of sensitization, these patients can be clustered in two groups. In 18 cases, hypersensitivity was caused by professional exposure in the manufacture of dyes or handling of spices and food additives [2, 5, 10, 11, 14,24,25,26], present report]. These occupational cases were mainly men (12/18), and among them an atopic background did not seem to be a predisposing factor, since only 3 out of the 18 displayed additional sensitivities.…”

Section: Discussionmentioning

confidence: 92%

“…In that paper, IgE from a sensitized worker of a spice warehouse bound high-molecular-weight proteins, although no accurate molecular weight estimations were available. Recently, Lizaso et al [11] reported the results of immunoblotting in 4 cases of occupational sensitization to cochineal in a dye factory. In their study, three proteins were responsible for IgE binding to carmine (28 and 50 kDa) and to cochineal (17, 28 and 50 kDa).…”

Section: Discussionmentioning

confidence: 99%

“…Protein remnants from the cochineal insect are thought to play an important role as carmine allergens, but no single allergen has been identified as a cause of sensitization [6,10,11,12]. In addition, the role of carminic acid as a potential hapten has been suggested in several reports [13, 14].…”

Section: Introductionmentioning

confidence: 99%

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Occupational Asthma to Carmine in a Butcher

Ferrer

Calle

Andréu

et al. 2005

Int Arch Allergy Immunol

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Hypersensitivity to carmine (E120) has been identified as a cause of food intolerance and occupational asthma. We present a case of occupational asthma following exposure to carmine in a manufacturer of sausages and review the literature. Case Report: A 42-year-old non-atopic male presented with a 5-year history of rhinoconjunctivitis and asthma on occupational exposure to food additive dusts. Symptoms increased after work. The patient had been exposed for more than 20 years. Methods: Skin prick tests were performed with a battery of common inhalant allergens and spices. Cochineal, carmine lake and additive mixes used by the patient were extracted and subsequently used for skin prick test, bronchial provocation and in vitro measurements (specific IgE, Western blot and chromatographic fractionation). Results: Prick tests were positive to carmine and carmine-containing additives; carmine-specific IgE and bronchial challenge tests were also positive (PC₂₀ = 0.0004 mg/ml and 1.6 kU/l). Western blot showed IgE binding to bands of about 30 kDa on cochineal extract and a diffuse pattern at 40–97 kDa on carmine. This result was confirmed by gel filtration chromatography and dot blot. Carmine completely inhibited IgE binding to cochineal extract. Discussion: Carmine is a potential sensitizer in an occupational setting: 18 cases of occupational asthma have been described to date. Carmine allergens are poorly defined; in general, proteins from cochineal not removed by the extraction process are considered as the main allergens in carmine. Our results are consistent with this, but show that these proteins may be subject to chemical modification.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Occupational Asthma to Carmine in a Butcher

Ferrer

Calle

Andréu

et al. 2005

Int Arch Allergy Immunol

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“…Hansen plant, the following have been reported to be associated with allergic asthma in other facilities: carmine [Rodriquez et al 1989;Lizaso et al 2000;Tabar-Purroy et al 2003], a-lactalbumin (found in whey) [Bernaola et al 1994], paprika [Sastre et al 1996], soy flour [Bush et al 1988], protease derived from Bacillus subtilis [Bernstein 1972;Franz et al 1971], and lipase from Aspergillus oryzae [Brant et al intRoduCtion (Continued) Health Hazard Evaluation Report 2007Report -0327-3083 2004. Individuals with stable preexisting asthma may experience worsening of their symptoms from workplace exposures.…”

Section: Allergic Diseases From Exposures To Enzymes and Other Organimentioning

confidence: 99%

Health hazard evaluation report: HETA-2007-0327-3083, findings from industrial hygiene air sampling, ventilation assessment, and a medical survey at a facility that manufactures flavorings, modified dairy products, and bacterial additives, Chr. Hansen, Inc., New Berlin, Wisconsin.

2009

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This Health Hazard Evaluation (HHE) report and any recommendations made herein are for the specific facility evaluated and may not be universally applicable. Any recommendations made are not to be considered as final statements of NIOSH policy or of any agency or individual involved. Additional HHE reports are available at http://www.cdc.gov/niosh/hhe/ The employer shall post a copy of this report for a period of 30 calendar days at or near the workplace(s) of affected employees. The employer shall take steps to insure that the posted determinations are not altered, defaced, or covered by other material during such period.

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“…En un estudio reciente investigamos los alergenos implicados en el asma ocupacional por carmín mediante inmunoblot 21 . Para ello se separaron mediante electroforesis en gel de poliacrilamida (SDS-PAGE) las proteínas de los extractos de cochinilla (calentado y sin calentar) y de carmín.…”

Section: Identificación De Alergenosunclassified