Identification of allergens from the mite <i>Blomia tropicalis</i>

Caraballo, Luis; Puerta, Leonardo; Martínez, Beatriz; Moreno, Liliana

doi:10.1111/j.1365-2222.1994.tb02743.x

Cited by 60 publications

(46 citation statements)

References 19 publications

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“…tropicalis, as well as Dermatophagoides pteronyssinus, has been implicated as one of the important causative agents of mite-related allergic diseases in many countries in South America and Southeast Asia, because patients with typical allergic symptoms, such as asthma, atopic dermatitis and rhinitis, in these areas are known to show positive skin responses and high levels of serum IgE against antigens of B. tropicalis 3,6,8,13,15,19,25 . These facts strongly suggest that B. tropicalis contains allergen(s) with the potential to induce systemic IgE responses in humans 4,5 . However, there were few experimental studies to demonstrate the Bt-specific IgE production by antigen exposure 24 .…”

Section: Blomia Tropicalismentioning

confidence: 98%

Intranasal sensitization with Blomia Tropicalis antigens induces allergic responses in mice characterized by elevated antigen-specific and non-specific serum ige and peripheral blood eosinophil counts

Takeda

Arakawa

Toma

et al. 2004

Rev. Inst. Med. trop. S. Paulo

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SUMMARYIn order to evaluate the potential allergenicity of Blomia tropicalis (Bt) antigen, IgE production of both specific and non-specific for Bt antigen was monitored in BALB/c mice after exposure to the antigen by nasal route. It was evidenced that B. tropicalis contains a functional allergen in its components. The allergenic components, however, when administered intranasally without any adjuvant, did not function to induce IgE response within a short period. On the other hand, intranasal inoculation of Bt antigens augmented serum IgE responses in mice pretreated by a subcutaneous priming injection of the same antigens. Inoculation of Bt antigen without subcutaneous priming injections induced IgE antibody production only when the antigen was continuously administered for a long period of over 24 weeks. Even when the priming injection was absent, the Bt antigen inoculated with cholera toxin (CT) as a mucosal adjuvant also significantly augmented the Bt antigen-specific IgE responses depending on the dose of CT co-administered. The present study also demonstrated that Bt antigen/CT-inoculated mice showed increased non-specific serum IgE level and peripheral blood eosinophil rates without noticeable elevations of the total leukocyte counts. The immunoblot analysis demonstrated 5 main antigenic components reactive to IgE antibodies induced. These components at about 44-64 kDa position were considered to be an important candidate antigen for diagnosis of the mite-related allergy.

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Section: Blomia Tropicalismentioning

confidence: 98%

Intranasal sensitization with Blomia Tropicalis antigens induces allergic responses in mice characterized by elevated antigen-specific and non-specific serum ige and peripheral blood eosinophil counts

Takeda

Arakawa

Toma

et al. 2004

Rev. Inst. Med. trop. S. Paulo

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“…Smith et al (7) have investigated the IgG and IgG1-4 antibody responses to Der p 2 in children from high and low exposure groups by ELISA, demonstrating that ≥90% of the children had Der p 2-specific IgG antibodies, although the levels of both total specific IgG and IgG1-4 antibodies were substantially higher in the high exposure group. Recently, we (8) demonstrated IgE, IgG1 and IgG4 antibody responses to B. tropicalis in patients with respiratory allergy, emphasizing that our patients seem to be more frequently sensitized to B. tropicalis high-molecular weight (54-104 kDa) components than to the low-molecular weight (11-15 kDa) allergens, which have been detected predominantly in other studies (9,10).…”

Section: Kc Almeida Et Almentioning

confidence: 99%

Responses of IgE, IgG1, and IgG4 to concanavalin A-binding Blomia tropicalis antigens in allergic patients

Almeida¹,

Silva²,

Gennari-Cardoso³

et al. 2006

Braz J Med Biol Res

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Blomia tropicalis (Bt) and Dermatophagoides pteronyssinus (Dp) are the prevalent house dust mites in tropical countries and are associated with allergic diseases. Glycosylated antigens are highly immunogenic and involved in different pathologies. We evaluated the presence of IgE, IgG1, and IgG4 to concanavalin A-binding antigens (Bt-Con-A) isolated from Bt-total extract in sera of allergic and non-allergic subjects. Bt-total and Bt-Con-A extracts were evaluated by SDS-PAGE and ELISA for reacting with IgE, IgG1, and IgG4 in sera of 121 patients with allergic rhinitis and 36 non-allergic individuals. All subjects were skin prick tested with Bt-total extract and inhibition tests were performed for IgE, IgG1, and IgG4 using both extracts (Bttotal and Bt-Con-A). Skin prick test showed that 58% of the patients were sensitized to Bt (Bt+), with 52% reactive to both mites (Bt and Dp) and 6% to Bt only. A broad spectrum of proteins (14-152 kDa) was visualized in Bt-total and components >27 kDa for the Bt-Con-A extract. ELISA showed a similar profile of IgE, IgG1 and IgG4 levels in response to Bt-total and Bt-Con-A extracts in different groups, although Bt+ patients showed a lower IgG4 reactivity to Bt-Con-A extract. Specific IgG1 levels were higher in Bt+ patients than in control subjects, and IgG4 levels showed no significant difference among groups. ELISA inhibition showed a partial IgE and total IgG1 and IgG4 cross-reactivity with Dp extract for Bt-total and Bt-Con-A extracts. We conclude that Con-A-binding components isolated from Bt constitute major allergens and are involved in both allergen sensitization (IgE response) and homeostasis maintenance (IgG1 and IgG4 responses).

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“…It is therefore important that doctors determine the allergens of their region. (306,(309)(310)(311)(312)(313)(314). Most mite allergens are associated with enzymatic activities (315) which were shown to have direct nonspecific action on the respiratory epithelium (316,317), some of which may potentiate a Th2 cell response (318).…”

Section: Allergen Exposurementioning

confidence: 99%