Identification of agr-positive methicillin-resistant Staphylococcus aureus harbouring the class A mec complex by MALDI-TOF mass spectrometry

Josten, Michaele; Dischinger, Jasmin; Szekat, Christiane; Reif, Marion; Al-Sabti, Nahed; Sahl, Hans‐Georg; Parčina, Marijo; Bekeredjian‐Ding, Isabelle; Bierbaum, Gabriele

doi:10.1016/j.ijmm.2014.07.005

Cited by 73 publications

(66 citation statements)

References 33 publications

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“…The mass peak at 3007.3 Da present in MRSA isolates was consistent with the expression of PSM-mec as previously reported [6], while the other two peaks were novel peaks which have never been previously identified. J.E.…”

Section: Discussionsupporting

confidence: 89%

Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) by the Vitek MS Saramis system

Shan

Fang

et al. 2015

Curr Microbiol

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A rapid, sensitive, and accurate Vitek MS assay was developed to distinguish clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) from clinical isolates of methicillin-sensitive Staphylococcus aureus (MSSA) by developing an in-house knowledgebase of SuperSpectra. Three unique peaks, including peaks at 2305.6 and 3007.3 Da specific to MRSA, and 6816.7 Da specific to MSSA, were selected for differentiating MRSA and MSSA. This assay accurately identified 84 and 91% of clinical MRSA and MSSA strains out of the total 142 clinically acquired S. aureus strains that were tested. This method will greatly improve the efficiency of single clinical sample identification of MRSA, thereby facilitating a reduction in the transmission of MRSA in clinical settings.

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Section: Discussionsupporting

confidence: 89%

Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) by the Vitek MS Saramis system

Shan

Fang

et al. 2015

Curr Microbiol

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“…Some mass peaks are associated with a resistance pattern of an isolate due to the expression of a specific protein involved in the directed or associated resistance phenotype and may be useful for differentiating between susceptible and resistant isolates. Although this technique has been successfully applied in some studies that are explained further below (81)(82)(83), it must be validated in clinical settings to ensure the reproducibility of the results. The main advantage of this methodology is its simplicity, as the sample preparation procedure is the same as or quite similar to that used for identification.…”

Section: Detection Of Antimicrobial Resistance By Analysis Of the Masmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

2018

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SUMMARY Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been successfully applied in recent years for first-line identification of pathogens in clinical microbiology because it is simple to use, rapid, and accurate and has economic benefits in hospital management. The range of clinical applications of MALDI-TOF MS for bacterial isolates is increasing constantly, from species identification to the two most promising applications in the near future: detection of antimicrobial resistance and strain typing for epidemiological studies. The aim of this review is to outline the contribution of previous MALDI-TOF MS studies in relation to detection of antimicrobial resistance and to discuss potential future challenges in this field. Three main approaches are ready (or almost ready) for clinical use, including the detection of antibiotic modifications due to the enzymatic activity of bacteria, the detection of antimicrobial resistance by analysis of the peak patterns of bacteria or mass peak profiles, and the detection of resistance by semiquantification of bacterial growth in the presence of a given antibiotic. This review provides an expert guide for MALDI-TOF MS users to new approaches in the field of antimicrobial resistance detection, especially possible applications as a routine diagnostic tool in microbiology laboratories.

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“…The psm-mec locus is found in the J2 region adjacent to mecI of the class A mec gene complex in several widespread HA-MRSA lineages, but most prominently in Sequence Type (ST) 5, ST36, ST45, ST225, and ST239 strains (Deurenberg and Stobberingh, 2009; Nübel et al, 2010; Castillo-Ramirez et al, 2012; Schulte et al, 2013), which all have either SCC mec types II or III (Chatterjee et al, 2011; Monecke et al, 2012; Josten et al, 2014). However, more detailed analysis revealed that the psm-mec locus is found in SCC mec types II, IIA, IIB, IID, III, and VIII of many Staphylococcus species (Monecke et al, 2012; Shore and Coleman, 2013).…”

Section: The Distribution Of the Psm-mec Locus In Sccmecmentioning

confidence: 99%

“…The production of the PSM-mec peptide, which can be identified in bacterial culture filtrates by high-pressure liquid chromatography/mass spectrometry (HPLC/MS) (Queck et al, 2009) or Matrix-assisted laser desorption/ionization Time of Flight (MALDI-TOF) analysis (Josten et al, 2014), is a reliable indicator of methicillin resistance. However, the restricted distribution of the psm-mec locus severely limits the potential use of HPLC-based detection of PSM-mec for routine clinical screening of methicillin resistance.…”

Section: Expression and Regulation Of The Psm-mec Peptidementioning

confidence: 99%

PSM-Mec—A Virulence Determinant that Connects Transcriptional Regulation, Virulence, and Antibiotic Resistance in Staphylococci

et al. 2016

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PSM-mec is a secreted virulence factor that belongs to the phenol-soluble modulin (PSM) family of amphipathic, alpha-helical peptide toxins produced by Staphylococcus species. All known PSMs are core genome-encoded with the exception of PSM-mec, whose gene is found in specific sub-types of SCCmec methicillin resistance mobile genetic elements present in methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci. In addition to the cytolytic translational product, PSM-mec, the psm-mec locus encodes a regulatory RNA. In S. aureus, the psm-mec locus influences cytolytic capacity, methicillin resistance, biofilm formation, cell spreading, and the expression of other virulence factors, such as other PSMs, which results in a significant impact on immune evasion and disease. However, these effects are highly strain-dependent, which is possibly due to differences in PSM-mec peptide vs. psm-mec RNA-controlled effects. Here, we summarize the functional properties of PSM-mec and the psm-mec RNA molecule and their roles in staphylococcal pathogenesis and physiology.

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Identification of agr-positive methicillin-resistant Staphylococcus aureus harbouring the class A mec complex by MALDI-TOF mass spectrometry

Cited by 73 publications

References 33 publications

Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) by the Vitek MS Saramis system

Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) by the Vitek MS Saramis system

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

PSM-Mec—A Virulence Determinant that Connects Transcriptional Regulation, Virulence, and Antibiotic Resistance in Staphylococci

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