Identification of Africanized Honey Bees (Hymenoptera: Apidae) Incorporating Morphometrics and an Improved Polymerase Chain Reaction Mitotyping Procedure

Nielsen, David I.; Ebert, Paul R.; Hunt, Greg J.; Guzmán-Novoa, Ernesto; Kinnee, Scott A.; Page, Robert E.

doi:10.1093/aesa/92.2.167

Cited by 40 publications

(34 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar disagreement between mitochondrial and nuclear markers occurs in Africanized honey bees (Lobo 1995) and in A. m. iberica (Cánovas 2008(Cánovas , 2011. In studies of subspecies identification, it was recommended to use mtDNA only for initial screening (Rortais et al 2011) or together with morphometrics or nuclear markers (Nielsen et al 1999;Pinto et al 2003). It is sometimes suggested that molecular methods are better than morphological ones (Page 1998); however, not all of them are suitable for discrimination of all honey bee subspecies (Sheppard et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Wing geometric morphometrics and microsatellite analysis provide similar discrimination of honey bee subspecies

Oleksa¹,

Tofilski²

2014

Apidologie

View full text Add to dashboard Cite

-Identification of honey bee (Apis mellifera) subspecies is important for their protection. It is also used by queen breeders to maintain some breeding lines. In this study, we compared three methods of subspecies identification based on the following: 17 microsatellite loci, COI-COII mitotypes and geometric morphometrics of forewing venation. The methods were used to classify colonies and workers from a mixed population of A. m. mellifera and A. m. carnica. There was highly significant correlation between results obtained using the three methods. More than three quarters of colonies were classified to the same subspecies by all three methods. The agreement was highest between microsatellites and morphometrics. More than 90 % of colonies were classified to the same subspecies by the two methods. There was also relatively high agreement (75 %) between microsatellites and morphometrics when workers were classified as pure subspecies or hybrids. In particular, one pure subspecies was never misclassified as other pure subspecies. The results presented here show that morphometrics can be used for detection of hybrids between A. m. mellifera and A. m. carnica. geometric morphometrics / subspecies discrimination / microsatellites / mtDNA / Bayesian clustering / Apis mellifera / phenotype-genotype correlation

show abstract

Section: Discussionmentioning

confidence: 99%

Wing geometric morphometrics and microsatellite analysis provide similar discrimination of honey bee subspecies

Oleksa¹,

Tofilski²

2014

Apidologie

View full text Add to dashboard Cite

show abstract

“…Three sets of primers were used for the amplification of 16s rDNA, CO I and ND 5 gene segments. The primers used for the 16s rDNA segment were 5-CAACATCGAGGTCGCAAACATC-3 and 3-AG-TTGGGACTATGTTTTCCATG-5 (Nielsen et al, 1994), for the CO I segment were 5-GATTACTTC-CTCCCTCATTA-3 and 3-AATAAGTCTGAT-AGGTCTAA-5 (Nielsen et al, 1999). For the ND 5 segment we designed primers based on the known mitochondrial genome of A. m. ligustica: 5-TCG-AAATGAATAGGATACAG-3 and 3-TTAGGAT-TTGGTAGAGTTGG-5.…”

Section: Methodsmentioning

confidence: 99%

Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR – RFLP analysis of three mtDNA segments

et al. 2005

View full text Add to dashboard Cite

-The genetic structure and phylogenetic relationships among six honey bee populations were studied using RFLP analysis on three PCR-amplified mtDNA gene segments (16s rDNA, CO I, and ND 5). The populations were sampled from various areas of Greece and Cyprus and correspond to Apis mellifera adami, A. m. macedonica, A. m. cecropia, and A. m. cypria races, based on origin (Ruttner, 1988). Seven, eight and seven restriction enzymes were found to have at least one recognition site at the 16s rDNA, CO I, and ND 5 segments, respectively. Seven different haplotypes were detected and diagnostic patterns enabled us to discriminate A. m. macedonica from the rest of the populations (races). The estimated net nucleotide sequence divergence among the populations examined was found to range from 0.00 to 1.18 with the highest value observed to be between A. m. macedonica and non-A. m. macedonica populations. The trees obtained (by UPGMA and Dollo parsimony methods) revealed that the most distant population was that of A. m. macedonica.Apis mellifera / honey bee / mtDNA / genetic variability / Greece

show abstract

“…In this way, parents can influence gene expression in their offspring in a parent-specific manner (Ferguson-Smith, 2011;Drewell et al, 2012). Thus far there is no direct evidence of parental imprinting in the honey bee, but there is growing evidence that parent-of-origin effects occur (Nielsen et al, 1999;Guzmán-Novoa et al, 2005;Oldroyd et al, 2014). In addition, the caste system may lead to evolutionary conflicts that could provide the conditions for strong selection for the evolution of male-and female-specific epigenetic imprints that would potentially enhance the reproductive success of the parent (Haig, 1992;Queller, 2003;Dobata and Tsuji, 2012;Drewell et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The dynamic DNA methylation cycle from egg to sperm in the honey beeApis mellifera

et al. 2014

View full text Add to dashboard Cite

In honey bees (Apis mellifera), the epigenetic mark of DNA methylation is central to the developmental regulation of caste differentiation, but may also be involved in additional biological functions. In this study, we examine the whole genome methylation profiles of three stages of the haploid honey bee genome: unfertilised eggs, the adult drones that develop from these eggs and the sperm produced by these drones. These methylomes reveal distinct patterns of methylation. Eggs and sperm show 381 genes with significantly different CpG methylation patterns, with the vast majority being more methylated in eggs. Adult drones show greatly reduced levels of methylation across the genome when compared with both gamete samples. This suggests a dynamic cycle of methylation loss and gain through the development of the drone and during spermatogenesis. Although fluxes in methylation during embryogenesis may account for some of the differentially methylated sites, the distinct methylation patterns at some genes suggest parentspecific epigenetic marking in the gametes. Extensive germ line methylation of some genes possibly explains the lower-than-expected frequency of CpG sites in these genes. We discuss the potential developmental and evolutionary implications of methylation in eggs and sperm in this eusocial insect species.

show abstract

Identification of Africanized Honey Bees (Hymenoptera: Apidae) Incorporating Morphometrics and an Improved Polymerase Chain Reaction Mitotyping Procedure

Cited by 40 publications

References 0 publications

Wing geometric morphometrics and microsatellite analysis provide similar discrimination of honey bee subspecies

Wing geometric morphometrics and microsatellite analysis provide similar discrimination of honey bee subspecies

Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR – RFLP analysis of three mtDNA segments

The dynamic DNA methylation cycle from egg to sperm in the honey beeApis mellifera

Contact Info

Product

Resources

About