Identification of Aeromonas strains to the genospecies level in the clinical laboratory

Abbott, Sharon L.; Cheung, Wendy K. W.; Kroske-Bystrom, S.; Malekzadeh, T.; Janda, J. Michael

doi:10.1128/jcm.30.5.1262-1266.1992

Cited by 130 publications

(70 citation statements)

References 21 publications

(24 reference statements)

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“…cephalotin. Similar results have been reported for this species by other authors (Altwegg et al 1990 ;Altwegg and Lüthy-In conclusion, the results of this study show that only a few phenotypic tests are useful for distinguishing Aeromonas Hottenstein 1991 ; Abbott et al 1992 ;Kämpfer and Altwegg 1992 ;Hänninen and Siittonen 1995). However, contrary to genomic species isolated from different sources.…”

Section: Discussionsupporting

confidence: 91%

“…Hottenstein (1991), Abbott et al (1992), Kämpfer and ----------------------------------------------------------------------- ------------------------------------------------------------------------ ---------------------------------------------------------------------- ------------------------------------------------------------------------ , Carnahan and Joseph (1993), Hänninen and lake water. Altwegg et al (1990) and Janda et al (1996) have suggested that infection resulting from environmental Siittonen (1995).…”

Section: Discussionmentioning

confidence: 99%

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Identification of Aeromonas strains of different origin to the genomic species level

Kaznowski

1998

J Appl Microbiol

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A . K AZ N OW SK I . 1998. A total of 71 Aeromonas strains was identified with established genomic species by DNA-DNA hybridization. The strains were isolated from diarrhoeal stools, dead and live fish, drinking, lake, river and sea water, municipal sewage and aluminium rolling emulsion. The strains were allocated to seven hybridization groups (HGs) but the majority belonged to HG 4 (42%), HG 8/10 (30%) and HG 3 (18%). All strains were examined by 136 phenotypic tests. Useful phenotypic characters for separation of Aeromonas HG 1-3 genospecies were: utilization of DLlactate, urocanic acid and growth at 40·5°C. Few phenotypic differences were detected between strains of HG 4, HG 5 and HG 6. Most isolates of the Aer. veronii biotype sobria (HG 8) showed a characteristic biochemical profile: positive V.P. (Voges-Proskauer) reaction, oxidation of gluconate, production of gas from glucose, susceptibility to cephalotin, no hydrolysis of elastin, arbutin and aesculin, and no acid production from L-arabinose, arbutin and salicin.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Identification of Aeromonas strains of different origin to the genomic species level

Kaznowski

1998

J Appl Microbiol

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“…Typing and intra-specific diversity studies require phenotypic and genetic tests [2,9,41] for an unequivocal strain identification to species level. Several previous typing and molecular epidemiological studies of A. hydrophila populations did not include a genetic method to confirm the identity of the strains [13,18,19].…”

Section: Discussionmentioning

confidence: 99%

Aeromonas hydrophilaclinical and environmental ecotypes as revealed by genetic diversity and virulence genes

Aguilera‐Arreola

Hernández-Rodríguez

Zúñiga

et al. 2005

FEMS Microbiology Letters

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Aeromonas hydrophila strains recovered from clinical samples and ambient sources were phenotypically and genetically identified. In addition, the distribution of putative virulence factors was assayed. To determine the genetic diversity of these strains, random amplification of polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC)-PCR markers were used. The discriminatory ability of the techniques, using Simpson's index, was 0.96 for both methods. The most consistent dendrogram was obtained when RAPD and ERIC data were combined. The genetic diversity revealed a high intra-specific genetic diversity (h=0.364+/-0.024 and I=0.538+/-0.030). The strains showed a tendency to cluster according to their origin of isolation (best-cut test 0.80 and bootstrap values >50%). The present study demonstrates and quantifies the high intra-specific diversity within this species and reveals a clear differentiation of strains according to their ecological origin. The distribution of virulence-related genes confirm that A. hydrophila is a genetically heterogeneous species that harbour ecotypes which have different pathogenic potential to human and other animals.

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“…Suspect colonies from the selective agars were identi®ed as previously described (Furniss et al 1979;Carnahan et al 1991;Abbott et al 1992;Janda et al 1995;McLaughlin 1995).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and virulence properties of Vibrio cholerae non-O1, Aeromonas spp. and Plesiomonas shigelloides isolated from Cambe Stream (State of Parana, Brazil)

Gibotti

Saridakis²,

Pelayo³

et al. 2000

J Appl Microbiol

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The incidence of Vibrio cholerae, Aeromonas spp. and Plesiomonas shigelloides was determined in water samples from Cambe Â Stream. The samples were collected from seven different sites. The serogroups, virulence markers and drug resistance pro®les were also evaluated. Twelve Aer. hydrophila, 12 Aer. caviae, eight Aer. sobria, seven Ple. shigelloides and two V. cholerae non-O1 were isolated. They belonged to different serogroups and all produced haemolysis in different assays. Five of the Aeromonas strains and one of V. cholerae non-O1 were positive for enterotoxin activity. Haemagglutination and its inhibition, using erythrocytes of different origins, was variable for Aeromonas spp. and V. cholerae, while none of the Ple. shigelloides haemagglutinated in association with any type of erythrocyte. All isolates exhibited multiple drug resistance. These results indicate that the occurrence of V. cholerae non-O1, Aeromonas spp. and Ple. shigelloides, in water used for vegetable irrigation, human recreation and animal consumption, among others, represents a potential risk for humans.

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Identification of Aeromonas strains to the genospecies level in the clinical laboratory

Cited by 130 publications

References 21 publications

Identification of Aeromonas strains of different origin to the genomic species level

Identification of Aeromonas strains of different origin to the genomic species level

Aeromonas hydrophilaclinical and environmental ecotypes as revealed by genetic diversity and virulence genes

Prevalence and virulence properties of Vibrio cholerae non-O1, Aeromonas spp. and Plesiomonas shigelloides isolated from Cambe Stream (State of Parana, Brazil)

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