Identification of Aeromonas hydrophila hybridization group 1 by PCR assays

Cascón, Alberto; Anguíta, Juan; Hernanz, Carmen; Sánchez, María de la Paz; Fernández, M; Naharro, Germán

doi:10.1128/aem.62.4.1167-1170.1996

Cited by 74 publications

(44 citation statements)

References 25 publications

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“…The presence of aerolysin, elastase, hemolysin, and lipase genes were verified by PCR using the specific primers were as described previously (Cascon, Anguita, & Hernanz, 1996;Dahdouh, Basha, Khalil, & Tanekhy, 2016;Howard, Garland, Green, & Buckley, 1987;Sen, 2005), and shown in The images were captured digitally and analyzed using a Gel Image system (Bio-Rad). and a final extension at 728C for 10 min.…”

Section: Detection Of Virulence Genes By Pcrmentioning

confidence: 99%

Antibiotic susceptibility and molecular characterization of Aeromonas hydrophila from grass carp

Yang

Miao

et al. 2017

Journal of Food Safety

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Aeromonas hydrophila is the leading cause of fish borne outbreaks and also represents a major threat to human health worldwide. This bacterium harbors many virulence factors such as aerolysin, elastase, and lipase genes. The extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant A. hydrophila. In the current study, we aimed to determine the incidence and features of A. hydrophila in grass carp in south China. We found 63 (31.5%) A. hydrophila isolates through investigation of 200 samples from five provinces in south China. All isolates were analyzed for virulence genes by polymerase chain reaction (PCR), antibiotic susceptibility analysis was carried out using the disk diffusion method, and molecular typing was performed using enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR). The results showed that the positive for aerolysin, elastase, hemolysin, and lipase gene were 71.43, 46.03, 0.00, and 55.56%, respectively. Antimicrobial susceptibility results indicated that most strains were resistant to ampicillin (95.24%), tetracycline (88.89%), and streptomycin (79.37%). A lot of multiple antibiotic resistance were found. The isolates were grouped into five clusters by ERIC-PCR and shown the genetic diversity. Thus, our findings demonstrated the presence of A. hydrophila from grass carp, provided insights into the dissemination of antibiotic-resistant strains, and improved our knowledge of microbiological risk assessment in this bacteria. Practical applicationsInvestigating the prevalence of Aeromonas hydrophilia isolates from grass carp could improved for assuring this microbial safety on food. In this study, antibiotic susceptibility, virulence genes, and molecular subtyping were selected to comprehensive analyze A. hydrophilia isolates. As grass carp are common and popular fish choices in China, therefore the continuous monitoring of this pathogens are vital to ensure the safety.

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Section: Detection Of Virulence Genes By Pcrmentioning

confidence: 99%

Antibiotic susceptibility and molecular characterization of Aeromonas hydrophila from grass carp

Yang

Miao

et al. 2017

Journal of Food Safety

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“…Members of the genus Aeromonas are ubiquitous in most aquatic environments (Holmes, Nicholls & Sartory 1996) The genus comprises a group of Gram-negative, facultatively anaerobic bacteria that are pathogenic for aquatic and terrestrial animals and have also been associated with a wide spectrum of infectious diseases in humans (Altwegg & Geiss 1989;Paniagua, Rivero, Anguita & Naharro 1990;Cascón, Anguita, Hernanz, Sánchez, Fernandez & Naharro 1996). Several species have been implicated in fish disease (e.g.…”

Section: Introductionmentioning

confidence: 99%

Incidence of class 1 integron and other antibiotic resistance determinants in Aeromonas spp. from rainbow trout farms in Australia

Ndi

Barton

2011

Journal of Fish Diseases

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There is limited information on antibiotic resistance determinants present in bacteria of aquaculture origin in Australia. The presence of integron and other resistance determinants was investigated in 90 Aeromonas isolates derived from nine freshwater trout farms in Victoria (Australia). Polymerase chain reaction was carried out for the detection of integrase genes Int1, Int2 and Int3, gene cassette array, integron-associated aadA, sul1 and qac1 genes, streptomycin resistance genes strA-strB, β-lactamase resistance genes bla(TEM) and bla(SHV) , and tetracycline resistance genes tetA-E and tetM. Clonal analysis was performed by pulsed-field gel electrophoresis (PFGE). Class 1 integrons were detected in 28/90 (31%) and class 2 and class 3 in none of the strains, aadA gene in 19/27 (70%) streptomycin-resistant strains, sul1 in 13/15 (86.7%) sulphonamide-resistant strains and qac1 gene in 8/28 (28.6%) integron-bearing strains. Five strains from two different farms carried gene cassettes of 1000 bp each containing the aadA2 gene and PFGE analysis revealed genetic relatedness. tetC was detected in all and tetA in 9/18 (50%) tetracycline-resistant strains. The strA-strB, bla(TEM) or bla(SHV) genes were not detected in any of the strains. Aeromonas spp. carrying integrons and other resistance genes are present in farm-raised fish and sediments even though no antibiotics were licensed for use in Australian aquaculture at the time of the study.

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“…The sensitivity of PCR analysis accords with that described for other bacteria, that is, between 1 and 20 CFU, or between 1 and 100 pg of DNA extracted from Staphylococcus spp. (3,4,17,36).…”

Section: Discussionmentioning

confidence: 99%

“…Molecular methods such as PCR-based DNA fingerprinting and hybridization have also been used successfully for staphylococcus identification at the species level (6,9,15,20,36). In general, rapid bacterial identification by either PCR or hybridization uses species-specific and ubiquitous DNA as a target (3,4). However, the use of universal pathway genes and universal function genes, whose nucleotide sequences are fairly homologous in bacteria, is becoming more and more frequent as target DNAs for PCR amplification (9,19,26).…”

mentioning

confidence: 99%

Glyceraldehyde-3-Phosphate Dehydrogenase-Encoding Gene as a Useful Taxonomic Tool for Staphylococcus spp

et al. 2000

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The gap gene of Staphylococcus aureus, encoding glyceraldehyde-3-phosphate dehydrogenase, was used as a target to amplify a 933-bp DNA fragment by PCR with a pair of primers 26 and 25 nucleotides in length. PCR products, detected by agarose gel electrophoresis, were also amplified from 12 Staphylococcus spp. analyzed previously. Hybridization with an internal 279-bp DNA fragment probe was positive in all PCR-positive samples. No PCR products were amplified when other gram-positive and gram-negative bacterial genera were analyzed using the same pair of primers. AluI digestion of PCR-generated products gave 12 different restriction fragment length polymorphism (RFLP) patterns, one for each species analyzed. However, we could detect two intraspecies RFLP patterns in Staphylococcus epidermidis, Staphylococcus hominis, and Staphylococcus simulans which were different from the other species. An identical RFLP pattern was observed for 112 S. aureus isolates from humans, cows, and sheep. The sensitivity of the PCR assays was very high, with a detection limit for S. aureus cells of 20 CFU when cells were suspended in saline. PCR amplification of the gap gene has the potential for rapid identification of at least 12 species belonging to the genus Staphylococcus, as it is highly specific.

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Identification of Aeromonas hydrophila hybridization group 1 by PCR assays

Cited by 74 publications

References 25 publications

Antibiotic susceptibility and molecular characterization of Aeromonas hydrophila from grass carp

Antibiotic susceptibility and molecular characterization of Aeromonas hydrophila from grass carp

Incidence of class 1 integron and other antibiotic resistance determinants in Aeromonas spp. from rainbow trout farms in Australia

Glyceraldehyde-3-Phosphate Dehydrogenase-Encoding Gene as a Useful Taxonomic Tool for Staphylococcus spp

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