Identification of active VQ motif-containing genes and the expression patterns under low nitrogen treatment in soybean

Wang, Xiaobo; Zhang, Haowei; Sun, Genlou; Yuan, Jing; Qiu, Lijuan

doi:10.1016/j.gene.2014.04.012

Cited by 45 publications

(60 citation statements)

References 17 publications

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“…The large number of VQ proteins isolated is consistent not only with the VQ domain being a WRKY (Group IIc or I) interaction domain but also with reports that WRKY proteins can typically interact with many VQ proteins. 11 The soybean VQ protein family has previously been studied by Wang et al 31 and 74 VQ (GmVQ 1-74) motif-containing genes were found 31 . Our data illustrated that that GmWRKY53…”

Section: Resultsmentioning

confidence: 99%

“…The importance of disease resistance, abiotic stress, and the clock make further analysis of the GmWRKY53/LHY interaction a priority. Soybean contains at least 74 VQ proteins 31 and nineteen of these were isolated as GmWRKY53-interacting proteins in the Y2H screen (Table 1). Similar data with the Arabidopsis AtWRKY51 protein showed that about half of the 34 Arabidopsis VQ proteins are interactors 11 .…”

Section: Group Iic and Group I Wrky Transcription Factors Our Data Omentioning

confidence: 99%

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The interactome of soybean GmWRKY53 using yeast 2-hybrid library screening to saturation

Tripathi

Rabara²,

Choudhary³

et al. 2015

Plant Signaling & Behavior

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Soybean GmWRKY53 functions in both biotic and abiotic stress signaling. Using GmWRKY53 as a bait yeast 2-hybrid library screening to saturation isolated multiple independent fragments for many interacting proteins, enabling delineation of minimal interacting domains and computation of a confidence score. Multiple independent clones coding for the LATE ELONGATED HYPOCOTYL clock protein GmLCL2 (MYB114) were isolated and the binding site for GmWRKY53 was mapped to 90 amino acids separate from the MYB domain. This suggests a direct input from the clock on GmWRKY53 activity. The GmWRKY53-interacting proteins also included 3 water stress-inducible AP2/ERF transcription factors. One of these (Glyma03g26310) is one of the most strongly water stress induced genes in soybean roots, suggesting that GmWRKY53/ERF complexes regulate water stress responses.

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Section: Resultsmentioning

confidence: 99%

Section: Group Iic and Group I Wrky Transcription Factors Our Data Omentioning

confidence: 99%

The interactome of soybean GmWRKY53 using yeast 2-hybrid library screening to saturation

Tripathi

Rabara²,

Choudhary³

et al. 2015

Plant Signaling & Behavior

View full text Add to dashboard Cite

show abstract

“…All plants were cultivated in a growth chamber at 22–25 °C (day 25 °C/night 22 °C) under long‐day (16 h light/8 h dark) conditions. To investigate the effects of low nitrogen treatment on the SOD isoenzyme activity and GmCZ‐SOD gene expression, seedlings were then transferred to a solution containing 15% nitrogen and cultivated for 3–4 days as previously described . Subsequently, the seedlings were harvested and immediately frozen in liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

“…qRT‐PCR was performed using SYBR Premix Ex Taq™II (TaKaRa, Toyoto, Japan) on an ABI Prism 7000 sequence detection system (Applied Biosystems, Grand Island, NY, USA) with the primers listed in Table . The mRNA level was normalized by ACT11 as previously described . The amplification results for RT‐PCR was calculated as 2 −ΔΔCt method, according to the previous description …”

Section: Methodsmentioning

confidence: 99%