Identification of active ingredients mediating anti-platelet aggregation effects of BuyangHuanwu decoction using a platelet binding assay, solid phase extraction, and HPLC-MS/MS

Liao, Fengyun; Yu, Aiming; Yu, Jianye; Wang, Ding; Wu, Yinai; Zheng, Huazhu; Meng, Yingjiao; Dan, He; Shen, Xiao; Wang, Lisheng

doi:10.1016/j.jchromb.2018.06.027

Cited by 14 publications

(7 citation statements)

References 32 publications

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“…To further determine the chemical structures of the compounds, the data was compared with the standard product and literature. The MS data of calycosin-7-O-glucoside, 14 calycosin, 15 formononetin, 16 cinnamic alcohol, 17 cinnamic acid, 18 betaine, 19 wogonoside, 20 and paeoniflorin 21 were consistent with previous reports. The structures of dl-2-phenylpropionic acid, 6-methylcoumarin, and 4-hydroxycinnamic acid were determined in comparison with the standard product.…”

Section: Resultssupporting

confidence: 90%

“…With the help of Peak View software, a total of eleven compounds were identified. 14 Among them, eight compounds were identified under the positive ion mode, namely calycosin-7-O-glucoside (C 22 H 22 O 10 ), calycosin (C 16 H 12 O 5 ), formononetin (C 16 H 12 O 4 ), cinnamic alcohol (C 9 H 10 O), cinnamic acid (C 9 H 8 O 2 ), betaine (C 5 H 11 NO 2 ), dl-2-phenylpropionic acid (C 9 H 10 O 2 ), and 6-methylcoumarin (C 10 H 8 O 2 ); three components were identified in negative ion mode, including 4-hydroxycinnamic acid (C 9 H 8 O 3 ), wogonoside (C 22 H 20 O 11 ), and paeoniflorin (C 23 H 28 O 11 ). The secondary mass spectrum was shown in Figure 5 and Table 2 .…”

Section: Resultsmentioning

confidence: 99%

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Identification of Active Ingredients of Huangqi Guizhi Wuwu Decoction for Promoting Nerve Function Recovery After Ischemic Stroke Using HT22 Live-Cell-Based Affinity Chromatography Combined with HPLC-MS/MS

He¹,

Zheng²,

Li³

et al. 2021

DDDT

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Objective The Chinese medicine Huangqi Guizhi Wuwu Decoction (HGWD) has been reported to improve the clinical symptoms and restore nerve function after ischemic stroke; however, its active ingredients are not well-determined. Therefore, this study aimed to investigate the bioactive compounds of HGWD and explore the possible mechanism of action. Methods The methods, including live HT22 cells, solid-phase extraction, and HPLC-MS/MS were utilized. The potential ingredients were identified through comparisons with literature and monomer compounds. Then, oxygen-glucose deprivation reperfusion (OGD/R)-treated HT22 cells were utilized to investigate the effect of HGWD components with specific binding affinities. Reactive oxygen species (ROS), superoxide dismutase (SOD), lactate dehydrogenase (LDH), and Tunel staining were used as testing indexes to analyze the protective effects of potential active ingredients on OGD/R-induced damage. Results Eleven compounds with specific binding affinities were identified as calycosin-7-O-glucoside, calycosin, formononetin, cinnamic alcohol, cinnamic acid, betaine, dl-2-phenylpropionic acid, 4-hydroxycinnamic acid, 6-methylcoumarin, wogonin, and paeoniflorin. Among them, six compounds had a protective effect on OGD/R-treated HT22 cells. Furthermore, calycosin-7-O-glucoside, calycosin, paeoniflorin, 4-hydroxycinnamic acid, wogonin, and formononetin could regulate oxidative stress and apoptosis to attenuate the cell damage caused by OGD/R. Conclusion The mechanism of action of HGWD to promote neurological recovery after ischemic stroke was related to the regulation of oxidative stress and apoptosis. This study suggested that cell membrane affinity chromatography combined with HPLC-MS/MS could be applied to screen potential active components in traditional Chinese medicines (TCM).

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Identification of Active Ingredients of Huangqi Guizhi Wuwu Decoction for Promoting Nerve Function Recovery After Ischemic Stroke Using HT22 Live-Cell-Based Affinity Chromatography Combined with HPLC-MS/MS

He¹,

Zheng²,

Li³

et al. 2021

DDDT

Self Cite

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“…A review of the literature revealed the antithrombotic activities of many other decoctions were reported, such as Sheng Hua decoction, Buyang Huangwu decoction, Yiqi Huoxue decoction, etc. (Qian and Yu 2011;Liao et al 2018;Wu et al 2019). Their activities were relatively moderate with antiplatelet aggregation of 20-40%, similar to HLJDD.…”

Section: Resultsmentioning

confidence: 99%

Antithrombotic effects of Huanglian Jiedu decoction in a rat model of ischaemia-reperfusion-induced cerebral stroke

Liu

Chen

Liu

et al. 2021

Pharmaceutical Biology

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Context: Huanglian Jiedu Decoction (HLJJD) has a variety of pharmacological activities, such as antiinflammatory and neuroprotection against ischaemic brain injury. Objectives: This ex vivo study explores its antithrombosis activity and inhibition of platelet aggregation. Material and methods: To study the antithrombosis activity of HLJJD ex vivo, saline, or HLJDD (100, 200, and 500 mg/kg) was treated prophylactically by gavage for 3 days in Wistar rats (n ¼ 4). Based on the rat model of transient middle cerebral artery infarction (MCAO) or normal rats (n ¼ 4), the antithrombotic activity in the normal group and HLJDD subgroups on prothrombin time, thrombus weight, platelet aggregation, and others was evaluated, followed by the antiplatelet aggregation of its main components (n ¼ 4). Results: The weight of the thrombus increased significantly at 24 h after MCAO onset. HLJJD did not influence the change of PT, but significantly inhibited thrombosis by 12.5, 20.0, and 20.5% in reducing the dry weight of thrombus, and blocked collagen-induced platelet aggregation by 25.5, 39.0, and 42.7% and adhesion of blood platelet by 17.3, 26.2, and 27.3%. The IC 50 value of HLJJD on collagen-induced platelet aggregation was 670 mg/kg. Geniposide only facilitated antiplatelet aggregation induced by collagen, but not AA or ADP. Both baicalin and berberine showed markedly antiplatelet aggregation induced by all activators. The antithrombotic activity of baicalin was relatively higher than that of berberine (35.0-47.8% vs. 20.6-33.5%). Conclusion:Our results indicated that HLJDD regulated blood circulation by inhibiting platelet aggregation and thrombosis, which might also extensively contribute to the clinical prevention and treatment of cerebrovascular diseases.

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“…Nowadays, it is thought that galloyl and benzoyl moieties, which are distributed in some natural catechins in tea, are the major determinants of anticancer, antimicrobial, and antioxidant effects (Silva et al, 2002;Taguri et al, 2006;Rivero-Buceta et al, 2015). Recently, GPF has been shown to possess the antitumor (Zheng et al, 2018;Gao et al, 2020), antiplatelet aggregation (Liao et al, 2018b), anticoagulation (Koo et al, 2010), antiallergic potential (Shi et al, 2016), as well as angiogenic effects (Liao et al, 2018a). Besides, it can reduce the inflammatory response, cellular injury, and apoptosis in vitro (Yao et al, 2013;Song et al, 2014;Wen et al, 2018;Lu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

6′-O-Galloylpaeoniflorin Attenuates Osteoclasto-genesis and Relieves Ovariectomy-Induced Osteoporosis by Inhibiting Reactive Oxygen Species and MAPKs/c-Fos/NFATc1 Signaling Pathway

et al. 2021

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Emerging evidence suggests bright prospects of some natural antioxidants in the treatment of osteoporosis. 6′-O-Galloylpaeoniflorin (GPF), an antioxidant isolated from peony roots (one of very widely used Oriental medicines, with various anti-inflammatory, antitumor, and antioxidant activities), shows a series of potential clinical applications. However, its effects on osteoporosis remain poorly investigated. The current study aimed to explore whether GPF can attenuate osteoclastogenesis and relieve ovariectomy-induced osteoporosis via attenuating reactive oxygen species (ROS), and investigate the possible mechanism. After the culture of primary murine bone marrow-derived macrophages/monocytes were induced by the use of macrophage colony-stimulating factor (M-CSF) and the receptor activator of NF-κB ligand (RANKL) and then treated with GPF. Cell proliferation and viability were assessed by Cell Counting Kit-8 (CCK-8) assay. Thereafter, the role of GPF in the production of osteoclasts and the osteogenic resorption of mature osteoclasts were evaluated by tartrate-resistant acid phosphatase (TRAP) staining, podosome belt formation, and resorption pit assay. Western blotting and qRT-PCR examination were performed to evaluate proteins’ generation and osteoclast-specific gene levels, respectively. The ROS generation in cells was measured in vitro by 2′,7′-Dichlorodi-hydrofluorescein diacetate (DCFH-DA). Ovariectomy-induced osteoporosis mouse administered with GPF or vehicle was performed to explore the in vivo potential of GPF, then a micro-CT scan was performed in combination with histological examination for further analysis. GPF suppressed the formation of osteoclasts and podosome belts, as well as bone resorption when induced by RANKL through affecting intracellular ROS activity, MAPKs signaling pathway, and subsequent NFATc1 translocation and expression, as well as osteoclast-specific gene expression in vitro. In vivo study suggested that exposure to GPF prevented osteoporosis-related bone loss in the ovariectomized mice. These findings indicate that GPF attenuates osteoclastogenesis and relieves ovariectomy-induced osteoporosis by inhibiting ROS and MAPKs/c-Fos/NFATc1 signaling pathway. This suggested that GPF may be potentially used to treat bone diseases like periodontitis, rheumatoid arthritis, and osteoporosis associated with osteoclasts.

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Identification of active ingredients mediating anti-platelet aggregation effects of BuyangHuanwu decoction using a platelet binding assay, solid phase extraction, and HPLC-MS/MS

Cited by 14 publications

References 32 publications

Identification of Active Ingredients of Huangqi Guizhi Wuwu Decoction for Promoting Nerve Function Recovery After Ischemic Stroke Using HT22 Live-Cell-Based Affinity Chromatography Combined with HPLC-MS/MS

Identification of Active Ingredients of Huangqi Guizhi Wuwu Decoction for Promoting Nerve Function Recovery After Ischemic Stroke Using HT22 Live-Cell-Based Affinity Chromatography Combined with HPLC-MS/MS

Antithrombotic effects of Huanglian Jiedu decoction in a rat model of ischaemia-reperfusion-induced cerebral stroke

6′-O-Galloylpaeoniflorin Attenuates Osteoclasto-genesis and Relieves Ovariectomy-Induced Osteoporosis by Inhibiting Reactive Oxygen Species and MAPKs/c-Fos/NFATc1 Signaling Pathway

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